Polr2b (NM_153798) Mouse Tagged ORF Clone

CAT#: MG211738

Polr2b (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide B (Polr2b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polr2b
• Synonyms: Pol2rb; Rpb2; Rpb140
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211738 representing NM_153798
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGACGCCGACGAGGACATGCAATATGATGAAGACGACGATGAGATCACTCCGGATCTGTGGCAGG
  AAGCATGCTGGATCGTGATCAGTTCTTACTTTGATGAGAAAGGCTTGGTCAGACAACAGCTGGATTCCTT
  TGATGAGTTTATTCAGATGTCTGTTCAAAGAATTGTGGAAGATGCACCACCGATTGACCTACAGGCCGAA
  GCTCAGCACGCCAGTGGAGAAGTTGAAGAGCCGCCAAGATATTTACTGAAGTTTGAACAAATTTACCTCT
  CCAAACCTACTCATTGGGAAAGAGATGGTGCCCCTTCGCCAATGATGCCAAATGAAGCCAGGTTAAGAAA
  TCTCACGTATTCTGCTCCGCTTTACGTTGACATAACAAAGACAGTCATTAAAGAAGGTGAAGAACAGCTG
  CAGACTCAGCACCAGAAAACGTTCATAGGGAAAATTCCAATTATGCTGCGCTCCACCTACTGCCTGTTAA
  ATGGCTTAACAGATCGTGACCTGTGTGAGCTGAATGAGTGTCCTTTGGATCCCGGTGGCTATTTTATTAT
  CAATGGATCAGAAAAGGTTCTGATAGCTCAAGAGAAAATGGCAACAAACACAGTCTATGTGTTTGCCAAA
  AAGGATTCCAAGTATGCCTATACAGGAGAATGTCGCTCATGCCTGGAGAATTCTTCCCGACCCACCAGTA
  CTATATGGGTTAGCATGCTGGCCAGAGGAGGACAGGGTGCAAAGAAGAGTGCTATTGGCCAGCGCATCGT
  GGCAACGTTACCATACATCAAGCAAGAAGTTCCTATCATCATTGTGTTCAGAGCACTAGGCTTTGTGTCT
  GATAGAGATATTTTAGAACACATCATTTATGACTTCGAGGATCCAGAGATGATGGAAATGGTTAAACCAT
  CTCTCGATGAAGCGTTTGTCATCCAAGAGCAGAATGTTGCCTTAAACTTCATCGGTTCAAGAGGGGCAAA
  GCCTGGTGTTACTAAGGAGAAAAGAATTAAATATGCAAAAGAAGTCTTACAGAAAGAAATGCTCCCTCAC
  GTTGGTGTCAGTGATTTCTGTGAGACCAAAAAGGCCTATTTCTTGGGGTATATGGTTCATAGGTTGCTTC
  TAGCAGCCCTAGGCAGAAGAGAACTAGACGACAGAGATCACTATGGCAACAAGAGATTGGATCTTGCTGG
  GCCTCTGCTTGCATTCTTATTCAGAGGCATGTTTAAGAATTTGCTGAAAGAAGTACGAATCTATGCGCAG
  AAGTTTATTGATCGCGGGAAGGATTTTAACTTAGAGCTGGCAATCAAGACAAGGATCATATCTGATGGGC
  TAAAATACTCTTTAGCCACTGGGAACTGGGGTGACCAGAAGAAAGCTCATCAAGCCAGAGCTGGAGTATC
  CCAGGTATTGAACCGCCTGACGTTTGCATCTACTCTTTCTCACCTGCGTCGTCTAAACTCTCCTATTGGC
  AGAGATGGCAAGCTAGCGAAGCCCAGACAGTTGCATAATACCTTGTGGGGAATGGTGTGTCCTGCTGAAA
  CTCCAGAGGGCCATGCTGTAGGGCTTGTGAAGAATTTAGCCCTGATGGCTTATATTTCAGTTGGATCTCA
  ACCCTCTCCAATTTTGGAATTTTTAGAAGAATGGAGCATGGAAAATTTAGAAGAAATTTCTCCTGCAGCT
  ATTGCTGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATCCATAAAGACCCCGAACAACTCA
  TGAACACGCTAAGGAAGTTACGGCGGCAGATGGACATCATTGTATCCGAAGTTTCTATGATCAGAGATAT
  TCGAGAGCGGGAGATTCGGATCTATACAGATGCAGGCCGTATTTGTAGACCCCTTCTGATTGTGGAAAAG
  CAGAAGCTGCTTTTGAAGAAGAGACACATTGACCAGTTGAAAGAGAGAGAATATAACAACTACAGTTGGC
  AGGATCTGGTAGCCAGTGGGGTAGTAGAGTATATTGATACCCTGGAAGAGGAGACCGTGATGCTTGCAAT
  GACTCCAGATGATTTGCAGGAGAAGGAAGTAGCTTATTGTTCTACCTACACCCACTGTGAGATTCACCCA
  TCAATGATTCTTGGAGTCTGTGCATCTATTATTCCTTTTCCTGATCATAACCAGTCCCCTAGAAACACAT
  ACCAGTCTGCTATGGGCAAGCAGGCTATGGGAGTTTATATCACCAACTTCCATGTGCGGATGGACACCTT
  GGCCCATGTTCTCTACTATCCTCAGAAACCCCTTGTGACTACACGGTCTATGGAATATCTACGATTCAGA
  GAGCTGCCAGCAGGAATCAACTCAATTGTGGCCATTGCATCCTACACGGGCTATAATCAAGAAGACTCTG
  TTATCATGAATCGTTCAGCTGTGGATCGAGGCTTTTTCAGGTCTGTTTTCTACCGATCATACAAAGAACA
  AGAGTCTAAAAAGGGATTTGATCAAGAAGAAGTGTTTGAGAAGCCTACACGTGAGACATGCCAGGGAATG
  AGGCATGCCATCTATGAAAAGTTGGACGATGACGGTTTGATAGCTCCGGGGGTCCGAGTCTCCGGAGATG
  ACGTTATTATCGGCAAGACAGTCACCTTACCCGAGAACGAGGATGAGCTGGAGAGCACCAACAGGCGCTA
  CACCAAGAGGGACTGCAGCACTTTCCTCAGAACTAGTGAGACGGGCATCGTGGACCAGGTCATGGTCACT
  CTCAACCAGGAAGGATATAAATTTTGTAAAATAAGGGTGCGCTCTGTTAGAATTCCGCAGATTGGAGACA
  AATTTGCTAGCCGACATGGTCAGAAGGGAACTTGTGGTATTCAGTATAGACAAGAGGACATGCCGTTCAC
  TTGCGAAGGCATAACTCCTGACATCATCATAAACCCCCACGCCATCCCCTCCCGGATGACAATTGGTCAC
  TTGATCGAATGTCTCCAAGGGAAGGTCTCTGCCAACAAGGGTGAAATTGGTGACGCTACTCCATTTAATG
  ATGCTGTCAATGTGCAGAAGATTTCTAATCTTTTGTCTGACTATGGCTACCATCTCAGAGGGAACGAGGT
  GCTCTACAATGGATTCACTGGACGAAAAATCACATCACAGATTTTTATTGGCCCCACTTACTACCAGCGT
  TTGAAGCATATGGTGGATGACAAAATTCATTCTCGTGCTAGGGGACCCATTCAGATTCTTAACAGACAGC
  CCATGGAGGGCAGATCTCGTGATGGTGGCCTGCGCTTCGGAGAAATGGAACGAGACTGTCAAATTGCCCA
  CGGTGCAGCACAGTTTCTAAGAGAAAGACTGTTTGAAGCATCAGATCCGTATCAGGTTCATGTCTGTAAT
  CTTTGTGGAATAATGGCAATTGCTAACACGAGGACTCATACATATGAGTGCAGGGGATGCCGCAATAAAA
  CCCAGATTTCCTTGGTGCGAATGCCGTACGCGTGCAAGCTGTTGTTCCAGGAGCTCATGTCCATGAGCAT
  CGCACCACGAATGATGAGTGTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211738 representing NM_153798
  Red=Cloning site Green=Tags(s)
  
  MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAE
  AQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQL
  QTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAK
  KDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVS
  DRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPH
  VGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ
  KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIG
  RDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAA
  IADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEK
  QKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHP
  SMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFR
  ELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGM
  RHAIYEKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVT
  LNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGH
  LIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQR
  LKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCN
  LCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153798
• ORF Size: 3522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153798.2, NP_722493.2
• RefSeq Size: 3812
• RefSeq ORF: 3525
• Locus ID: 231329