Trpm4 (BC096475) Mouse Tagged ORF Clone

CAT#: MG211808

Trpm4 (GFP-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (cDNA clone MGC:106240 IMAGE:4225167), complete

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trpm4
• Synonyms: TRPM4B, FLJ20041
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211808 representing BC096475
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGCCGGAGAAGGAGCAGAGCTGGATCCCTAAGATCTTCAGGAAGAAGGTGTGCACGACGTTCA
  TAGTGGACCTCTCCGATGATGCGGGAGGGACCTTGTGCCAGTGTGGGCAACCTCGGGATGCCCACCCTTC
  TGTGGCTGTGGAGGATGCCTTTGGGGCAGCCGTAGTTACCGAGTGGAACAGTGATGAGCACACCACGGAG
  AAGCCCACAGATGCCTATGGGGACCTGGACTTCACGTACTCTGGCCGAAAGCACAGCAACTTTCTCCGGC
  TGTCTGACCGCACGGATCCGGCCACTGTGTACAGTCTCGTTACCCGCTCTTGGGGCTTTCGTGCCCCAAA
  CTTGGTGGTGTCGGTGCTGGGGGGGTCGGGGGGCCCCGTGCTCCAGACCTGGCTGCAGGACCTTCTGCGT
  CGCGGGCTGGTGAGAGCCGCCCAGAGCACAGGGGCCTGGATCGTCACTGGGGGACTGCACACAGGCATTG
  GCCGGCACGTGGGTGTGGCTGTAAGGGACCACCAGACAGCCAGCACTGGGAGCAGCAAGGTGGTGGCCAT
  GGGTGTGGCCCCCTGGGGAGTGGTCCGGAACAGAGACATGCTGATCAACCCCAAGGGCTCATTCCCCGCA
  AGGTACCGGTGGCGTGGTGACCCTGAGGATGGTGTTGAGTTCCCCCTGGACTATAACTATTCTGCTTTCT
  TCTTGGTGGATGATGGCACCTATGGCCGCCTGGGTGGTGAGAACCGCTTCCGCCTTCGGTTTGAGTCCTA
  TGTGGCTCAGCAGAAGACCGGAGTGGGAGGGACTGGAATTGACATCCCAGTGCTCCTTCTTCTCATCGAT
  GGAGATGAGAAGATGTTAAAGCGGATAGAGGATGCCACCCAAGCTCAGCTCCCCTGCCTCTTGGTGGCGG
  GCTCTGGGGGTGCTGCAGACTGTCTGGTGGAGACCCTGGAAGATACTCTGGCCCCAGGGAGTGGGGGACT
  TAGGCGAGGCGAAGCCCGGGATCGGATTAGGCGATACTTCCCTAAAGGAGACCCTGAGGTTCTTCAGGCC
  CAGGTAGAGAGGATCATGACCCGAAAGGAGTTGCTGACGGTCTATTCATCAGAAGACGGCTCCGAGGAGT
  TTGAGACTATCGTTTTGAGGGCTCTTGTGAAAGCCTGTGGGAGCTCTGAGGCCTCAGCCTACCTGGATGA
  GCTGCGTTTGGCTGTGGCTTGGAACCGCGTGGACATCGCCCAAAGTGAACTTTTCCGTGGGGACATCCAA
  TGGCGGTCCTTCCACCTGGAGGCATCTCTCATGGATGCTCTGCTGAATGACCGGCCTGAATTTGTGCGCC
  TGCTCATCTCCCATGGCCTTAGCCTGGGACACTTCCTGACCCCCGTGCGTCTGGCCCAGCTGTACAGCGC
  GGTGTCCCCTAACTCACTGATCCGAAATCTTCTGGACCAGGCGTCCCATGCTAGTAGCAGCAAATCCCCT
  CCTGTAAATGGAACTGTAGAGCTCCGGCCTCCCAATGTGGGGCAAGTGCTGAGGACTCTGTTGGGAGAAA
  CGTGTGCACCACGGTACCCTGCCAGGAACACCCGAGACTCCTATCTGGGCCAGGACCACAGAGAGAATGA
  CTCTCTGCTTATGGACTGGGCCAACAAGCAACCATCAACGGATGCAAGCTTTGAACAAGCCCCCTGGAGT
  GACCTGCTTATTTGGGCTCTGTTGCTGAACCGGGCCCAGATGGCTATCTATTTCTGGGAGAAGGGCTCCA
  ACTCAGTGGCCTCTGCTCTTGGGGCTTGTCTTTTACTCCGAGTGATGGCTCGCCTAGAGTCGGAGGCTGA
  GGAGGCCGCGCGGCGGAAGGATCTTGCTGCCACGTTTGAAAGCATGAGCGTGGACCTCTTTGGAGAGTGT
  TACCACAACAGTGAAGAGCGAGCAGCCCGCCTTCTTCTGCGCCGCTGTCCCCTCTGGGGAGAGGCCACCT
  GCCTCCAGCTTGCCATGCAGGCTGATGCCCGAGCGTTCTTTGCCCAGGATGGAGTCCAGTCTCTTCTGAC
  ACAGAAGTGGTGGGGGGAGATGGACAGCACAACTCCCATCTGGGCCCTGCTTCTCGCCTTCTTCTGCCCG
  CCTCTCATCTACACCAACCTTATCGTCTTCAGGAAGTCAGAGGAGGAGCCCACACAGAAGGATCTTGACT
  TTGATATGGACAGCAGCATCAATGGAGCAGGTCCTCCTGGGACTGTAGAACCCTCTGCAAAGGTGGCCCT
  TGAGAGGCGGCAGCGGCGACGGCCAGGACGTGCCCTCTGCTGTGGCAAGTTCTCCAAGCGCTGGTCGGAC
  TTCTGGGGTGCCCCAGTGACTGCCTTCCTGGGTAATGTGGTCAGTTACCTGCTGTTCCTGCTGCTGTTTG
  CCCACGTGTTGCTGGTGGATTTCCAGCCCACAAAGCCAAGTGTCTCCGAGCTGCTGCTGTACTTCTGGGC
  CTTCACGTTGCTGTGCGAGGAGCTGCGCCAGGGCCTGGGTGGTGGCTGGGGTAGCCTGGCCAGTGGGGGA
  CGTGGTCCGGACAGAGCTCCCCTGCGCCATCGGCTGCACCTCTACCTCTCAGATACCTGGAACCAATGTG
  ACCTGCTGGCACTCACCTGCTTCCTGCTGGGCGTAGGCTGCCGGCTGACCCCTGGGCTCTTTGACTTGGG
  ACGCACCGTCCTCTGCCTTGACTTCATGATCTTCACACTGCGCCTGCTGCACATCTTCACGGTGAACAAG
  CAGCTGGGGCCCAAGATTGTCATAGTGAGCAAAATGATGAAGGATGTATTCTTCTTCCTCTTCTTCCTCT
  GCGTGTGGCTTGTAGCTTATGGGGTGGCCACAGAGGGGATCCTGAGGCCCCAGGACCGCAGTCTACCGAG
  TATCCTGCGCAGGGTCTTCTACCGACCGTATCTGCAGATCTTCGGACAAATTCCCCAGGAGGAAATGGAC
  GTGGCCCTCATGATTCCAGGTAACTGCTCAATGGAGCGGGGCTCCTGGGCTCATCCGGAGGGGCCCGTCG
  CAGGCTCCTGTGTGTCCCAGTATGCCAACTGGCTGGTGGTGTTGCTCCTTATCGTCTTCTTGCTGGTGGC
  CAATATCCTGTTGCTCAACCTGCTCATCGCCATGTTCAGCTACACCTTCAGCAAAGTGCACGGCAACAGC
  GACCTCTACTGGAAGGCACAGCGCTACAGCCTCATCCGGGAATTCCATTCGAGGCCTGCCCTGGCCCCAC
  CCCTCATCATCATCTCTCATGTGCGCCTCCTCATCAAGTGGCTGCGCAGGTGTCGCAGGTGTCGCAGGGC
  TAACTTGCCCGCCTCCCCAGTCTTCGAGCACTTCCGTGTCTGTCTCTCTAAGGAAGCAGAGCGGAAGCTG
  CTGACTTGGGAGTCTGTGCACAAAGAGAACTTCCTGTTGGCACAAGCTCGGGACAAGCGAGACAGTGACT
  CGGAGCGTTTGAAACGCACGTCTCAGAAGGTGGACACTGCACTGAAGCAGCTGGGACAGATCAGAGAGTA
  CGATCGGCGTCTGAGGGGGCTGGAGAGAGAGGTCCAACACTGTTCTCGAGTCCTGACTTGGATGGCTGAG
  GCCCTTAGCCACTCTGCCTTGCTGCCTCCAGGGGCCCCACCCCCTCCGAGCCCCACTGGTTCCAAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211808 representing BC096475
  Red=Cloning site Green=Tags(s)
  
  MVGPEKEQSWIPKIFRKKVCTTFIVDLSDDAGGTLCQCGQPRDAHPSVAVEDAFGAAVVTEWNSDEHTTE
  KPTDAYGDLDFTYSGRKHSNFLRLSDRTDPATVYSLVTRSWGFRAPNLVVSVLGGSGGPVLQTWLQDLLR
  RGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQTASTGSSKVVAMGVAPWGVVRNRDMLINPKGSFPA
  RYRWRGDPEDGVEFPLDYNYSAFFLVDDGTYGRLGGENRFRLRFESYVAQQKTGVGGTGIDIPVLLLLID
  GDEKMLKRIEDATQAQLPCLLVAGSGGAADCLVETLEDTLAPGSGGLRRGEARDRIRRYFPKGDPEVLQA
  QVERIMTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQ
  WRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSP
  PVNGTVELRPPNVGQVLRTLLGETCAPRYPARNTRDSYLGQDHRENDSLLMDWANKQPSTDASFEQAPWS
  DLLIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLESEAEEAARRKDLAATFESMSVDLFGEC
  YHNSEERAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTTPIWALLLAFFCP
  PLIYTNLIVFRKSEEEPTQKDLDFDMDSSINGAGPPGTVEPSAKVALERRQRRRPGRALCCGKFSKRWSD
  FWGAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPSVSELLLYFWAFTLLCEELRQGLGGGWGSLASGG
  RGPDRAPLRHRLHLYLSDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNK
  QLGPKIVIVSKMMKDVFFFLFFLCVWLVAYGVATEGILRPQDRSLPSILRRVFYRPYLQIFGQIPQEEMD
  VALMIPGNCSMERGSWAHPEGPVAGSCVSQYANWLVVLLLIVFLLVANILLLNLLIAMFSYTFSKVHGNS
  DLYWKAQRYSLIREFHSRPALAPPLIIISHVRLLIKWLRRCRRCRRANLPASPVFEHFRVCLSKEAERKL
  LTWESVHKENFLLAQARDKRDSDSERLKRTSQKVDTALKQLGQIREYDRRLRGLEREVQHCSRVLTWMAE
  ALSHSALLPPGAPPPPSPTGSKD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC096475
• ORF Size: 3641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC096475, AAH96475
• RefSeq Size: 4053
• RefSeq ORF: 3641
• Locus ID: 68667