Vars (NM_011690) Mouse Tagged ORF Clone

CAT#: MG211867

Vars (GFP-tagged) - Mouse valyl-tRNA synthetase (Vars)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vars
• Synonyms: Bat6; D17H6S56E; G7a; Vars2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211867 representing NM_011690
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATCCTCTACGTCTCTCCTCACCCCGACGCCTTCCCCAGCCTCCGAGCCCTCATCGCTGCCCGAT
  ACGGGGAAGCTGGTGACGGTCCCGGGTGGGGAGGCCCTCACCCCCGCATCTGCTTGCAGCCTCCCCCGAG
  CAGCCGGACTCCATTCCCTCCGCCTCGCCTGCCCGCCCTGGAGCAGGGACCTGGCGGCCTGTGGGTGTGG
  GGGGCCCCGGCTGTAGCTCAGCTGTTGTGGCCTGCAGGCCTGGGGGGACCCGGGGGCAGCCGGGCAGCTG
  TCCTGGTCCAGCAGTGGGTCAGTTATGCTGACACGGAGCTCATACCCGCTGCCTGCGGGGCGACCCTGCC
  TGCCCTGGGACTTCGAGGTCCTGGTCAGGATCCCCAGGCTGCCCTTGGGGCCCTGGGCAAGGCCTTGAAC
  CCCTTGGAGGACTGGCTGCGGCTGCACACCTACCTGGCTGGAGATGCCCCCACTCTGGCTGACTTAGCTG
  CTGTGACAGCCTTACTGCTGCCTTTCCGATACGTTCTGGACCCTTCTGCCCGCCGGATCTGGGGCAATGT
  GACTCGCTGGTTTAACACTTGTGTCCGGCAACCGGAATTCCGGGCTGTGCTGGGAGAAGTGGCCCTGTAC
  TCGGGGGCCAGGTCTGTCACTCAACAGCCAGGCTCTGAGGTCATCGCACCCCAAAAAACACCCGCGCAGC
  TCAAAAAAGAGGCAAAGAAACGGGAGAAACTAGAGAAATTCCAGCAGAAGCAGAAGACCCAGCAGCAGCC
  CCCGCACGGAGAGAAGAAACCAAAACCAGAGAAGAAGGAGAAACGGGACCCTGGGGTCATTACCTATGAC
  CTCCCTACCCCACCGGGGGAGAAGAAAGATGTAAGTGGCGCCATGCCCGACTCCTACAGCCCTCAGTATG
  TGGAGGCTGCCTGGTATCCATGGTGGGAGCGGCAGGGCTTCTTCAAGCCAGAGTACGGGCGTCCTAGTGT
  GTCAGCACCAAATCCCCGGGGTGTCTTCATGATGTGCATCCCACCCCCCAACGTGACAGGCTCCCTGCAC
  CTGGGCCACGCACTCACCAACGCCATCCAGGACTCCCTGACTCGATGGCACCGCATGCGTGGGGAGACCA
  CCCTATGGAACCCAGGCTGTGACCATGCAGGCATTGCCACCCAGGTGGTGGTGGAAAAGAAGCTCTGGAA
  AGAGCGGGGTCTGAACCGGCACCAGCTGGGCCGCGAGGCCTTTCTTGAGGAGGTCTGGAAGTGGAAAGCC
  GAGAAGGGTGACAGGATTTACCACCAATTAAAGAAACTTGGCAGCTCCCTGGACTGGGATCGAGCCTGCT
  TCACCATGGATCCTAAATTGTCAGCAACTGTGACAGAGGCCTTTGTTAGGCTCCACGAAGAAGGGGTCAT
  CTACCGTAGCACCCGCCTGGTCAACTGGTCCTGCACCCTCAACTCAGCCATTTCTGACATTGAGGTGGAT
  AAGAAGGAGCTGACAGGCCGCACCCTGCTTCCTGTGCCTGGCTACAAGGAGAAGGTGGAGTTTGGGGTCC
  TTGTGTCCTTTGCCTACAAGGTCCAAGGCTCAGACAGCGACGAGGAGGTGGTGGTGGCAACAACCCGGAT
  TGAGACCATGCTGGGAGACGTGGCTGTAGCTGTGCACCCCAAGGATCCCAGATACCAGCACTTAAAGGGA
  AAGTGTGTCGTCCACCCATTCTTGTCCCGGAGCCTTCCCATCGTCTTCGATGACTTTGTAGACATGGAGT
  TTGGCACAGGTGCCGTGAAGATCACCCCGGCACATGACCAAAATGACTATGAGGTTGGGCAGCGACACAG
  GCTCGAGGCCATTAGCATCATGGACTCAAAGGGGGCCCTCATCAATGTGCCTCCACCTTTCCTGGGCCTG
  CCCAGGTTTGAGGCCAGGAAGGCTGTGCTGGCGGCACTGAAGGAGCGGGGCCTGTTCCGTGGAATCAAGG
  ACAACCCCATGGTGGTGCCGCTTTGCAACCGCTCCAAGGATGTGGTGGAGCCTCTGTTAAGGCCACAATG
  GTACGTGCGCTGTGGGGAGATGGCTCAGGCTGCCAGTGCTGCTGTGACCCGGGGTGACCTCCGTATACTG
  CCTGAGGCCCATCAACGGACGTGGCATTCTTGGATGGACAACATCAGAGACTGGTGCATCTCTCGGCAGC
  TGTGGTGGGGCCACCGAATCCCTGCCTACTTTATCACCGTCCATGACCCGGCAGTACCCCCTGGGGAGGA
  CCCTGATGGGCGGTACTGGGTAAGCGGACGCACTGAAGCAGAGGCCCGAGAGAAGGCAGCACGGGAGTTT
  GGAGTGTCCCCTGACAAGATCAGCCTTCAGCAAGATGAGGATGTGTTGGACACCTGGTTCTCCTCCGGTC
  TCTTTCCCTTCTCCATCTTTGGCTGGCCCAATCAGTCAGAAGACCTCAGTGTGTTCTACCCTGGGACCCT
  GTTGGAGACAGGCCATGATATCCTCTTCTTCTGGGTGGCCCGGATGGTCATGCTCGGCCTGAAGCTCACT
  GGGAAGCTGCCCTTCAGAGAGGTCTATCTCCATGCAATCGTGCGTGATGCTCATGGCAGGAAGATGAGCA
  AGTCTCTTGGCAATGTCATCGACCCCCTGGATGTCATCCATGGAGTTTCCTTGCAGGGCCTCTATGACCA
  ACTACTGAACAGCAACCTGGATCCCAGTGAGGTGGAGAAAGCCAAAGAAGGACAGAAGGCCGACTTTCCA
  GCAGGGATTCCCGAGTGTGGCACTGATGCCCTACGCTTTGGACTCTGTGCCTACACATCCCAAGGTCGAG
  ACATCAACCTGGATGTGAACAGGATCCTGGGGTACCGTCACTTCTGCAACAAACTCTGGAATGCTACCAA
  GTTTGCCCTGCGCGGCCTTGGGAAGGGCTTTGTGCCCTCAGCAACCTCCAAGCCTGAAGGGCACGAGAGC
  CTGGTGGACCGCTGGATCCGCAGCCGCCTAACTGAGGCCGTGAGGCTCAGCAATGAAGGCTTCCAGGCTT
  ACGATTTCCCGGCCATCACCACCGCCCAGTACAGCTTTTGGCTCTATGAGCTCTGTGATGTCTATTTGGA
  GTGTCTAAAACCTGTGCTGAATGGAGTGGACCAGGTGGCGGCAGAGTGTGCTCGGCAGACCCTCTACACC
  TGCCTGGATGTTGGCCTGCGGCTGCTCTCGCCCTTCATGCCCTTTGTCACAGAGGAGCTATTCCAGAGAC
  TGCCCCGGCGGACACCAAAAGCGCCTGCTAGCCTCTGTGTCACCCCGTACCCAGAGCCCTCAGAGTGCTC
  CTGGAAGGACCCTGAAGCTGAAGCTGCTCTTGAACTAGCTCTGAGCATCACTCGAGCTGTGCGCTCCCTG
  CGTGCTGACTACAACCTGACCCGCACCAGGCCCGACTGTTTCTTGGAAGTAGCCGATGAGGCCACAGGTG
  CCTTGGCCTCGGCAGTGTCGGGCTACGTGCAGGCTCTGGCCAGTGCGGGCGTGGTGGCTGTCCTCGCCCT
  GGGTGCTCCTGCACCGCAGGGCTGCGCTGTAGCTGTGGCTTCTGACCGCTGCTCCATCCACCTGCAGCTG
  CAGGGGCTAGTGGACCCAGCCCGGGAGTTGGGCAAGCTGCAGGCCAAGCGAAGTGAGGCACAGCGGCAGG
  CTCAGCGGCTGCAGGAGCGCCGTGCTGCCTCCAGCTACTCAGCAAAGGTGCCCCTTGAGGTCCAGGAGGC
  AGATGAAGCGAAGTTGCAACAGACAGAGGCGGAGCTCAGGAAGGTGGATGAGGCCATCGCCCTGTTCCAG
  AAGATGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211867 representing NM_011690
  Red=Cloning site Green=Tags(s)
  
  MSILYVSPHPDAFPSLRALIAARYGEAGDGPGWGGPHPRICLQPPPSSRTPFPPPRLPALEQGPGGLWVW
  GAPAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRGPGQDPQAALGALGKALN
  PLEDWLRLHTYLAGDAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFNTCVRQPEFRAVLGEVALY
  SGARSVTQQPGSEVIAPQKTPAQLKKEAKKREKLEKFQQKQKTQQQPPHGEKKPKPEKKEKRDPGVITYD
  LPTPPGEKKDVSGAMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLH
  LGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFLEEVWKWKA
  EKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVD
  KKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKG
  KCVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALINVPPPFLGL
  PRFEARKAVLAALKERGLFRGIKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRIL
  PEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAAREF
  GVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLT
  GKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYDQLLNSNLDPSEVEKAKEGQKADFP
  AGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSATSKPEGHES
  LVDRWIRSRLTEAVRLSNEGFQAYDFPAITTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYT
  CLDVGLRLLSPFMPFVTEELFQRLPRRTPKAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSL
  RADYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVASDRCSIHLQL
  QGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEAKLQQTEAELRKVDEAIALFQ
  KML
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011690
• ORF Size: 3789 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011690.2, NP_035820.2
• RefSeq Size: 4021
• RefSeq ORF: 3792
• Locus ID: 22321