Col11a1 (NM_007729) Mouse Tagged ORF Clone

CAT#: MG212085

Col11a1 (GFP-tagged) - Mouse procollagen, type XI, alpha 1 (Col11a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Col11a1
• Synonyms: C530001D20Rik; cho
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212085 representing NM_007729
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCTGGTCCAGGTGGAAAACGAAACGGTGGATCTGGGATCTCACCATCTCAACCCTCGCCTTGA
  CTTTTCTCTTCCAAGCTAGAGAGGTCAGAGGAGCTGCTCCAGTTGATATACTAAAAGCATTAGATTTTCA
  CAATTCTCCAGTGGGAATATCTAAAACAACAGGATTTTGCACAAACAGAAAGAATTCTAAAGATCCAGAT
  GTTGCTTATAGAGTTACGGAGGAAGCACAAATCAGCGCCCCAACAAAGCAGCTATTTCCAGGAGGAATTT
  TTCCACAAGATTTTTCAATACTATTTACAATAAAACCCAAAAAAGGAACTCAAGCTTTCCTCTTGTCCCT
  CTACAATGAACATGGCATTCAGCAACTCGGTGTTGAGGTTGGGAGATCGCCCGTTTTTCTATTTGAAGAC
  CACACTGGAAAACCCACACCGGAAAACTATCCTCTCTTCTCAACAGTTAACATTGCTGATGGCAAGTGGC
  ACCGGGTCGCAATCAGTGTGGAGAAGAAAACTGTGACAATGATTGTTGATTGTAAGAAGAAAATCACAAA
  ACCCCTCGATAGAAGTGAGAGATCGATAGTCGATACCAATGGAATCATGGTATTTGGAACAAGAATTTTA
  GAAACAGATGTTTTCCAGGGTGATATTCAGCAGTTCCTGATCACAGGAGACCCCAAGGCAGCATACGACT
  ACTGTGACCATTACAGTCCAGATTGTGACTTAACATCCAAGGCTGCCCAGGCTCAGGAGCCTCACATTGA
  TGAGTATGCACCTGAGGATATAATCGAGTATGACTATGAATATGGGGAGACAGACTATAAAGAGGCTGAG
  AGTGTAACAGAGATGCCTACTTTTACTGAAGAAACAGTAGCACAAACAGAGGCAAATATTGTGGATGATT
  TTCAAGACTACAACTATGGAACAATGGAACCTTACCAGACTGAGACTCCTAGGCGGGTATCAGGATCGAA
  TGAGCCAAATCCAGTTGAAGAAGGTTTTACTGAAGAATATCTAACCGGAGAGGATTATGATGTCCAGAGA
  AACACTTCCGAGGATATTCTGTACGGGAACAAAGGAGTAGACGGCAGGGATTCTGATCTCCTGGTAGATG
  GAGATTTAGGTGAATATGATTTTTATGAATACAAAGAATATGAAGAAAGGACTACGACCTCCCCTAATGA
  AGAATTTGGCCCAGGTGTCCCAGCAGAAACTGATTTCACAGAAACAAGCATAAATGGACATGGTGCATAT
  GGAGAGAAAGGGCAGAAGGGGGAACCGGCTGTGGTTGAACCCGGGATGCTTGTTGAAGGACCACCTGGGC
  CAGCAGGACCAGCGGGTCTTATGGGTCCTCCAGGGCTACAAGGTCCTTCTGGGCTTCCCGGCGACCCTGG
  GGATAGGGGCCCACCAGGACGTCCTGGCTTACCAGGGGCTGATGGTTTACCTGGACCTCCTGGAACCATG
  CTGATGTTACCATTCCGCTATGGGGGTGACGGCTCCAAAGGACCAACAATCTCTGCACAGGAAGCCCAAG
  CTCAGGCGATTCTTCAGCAGGCTCGGATTGCTCTGAGAGGCCCTCCTGGCCCAATGGGTCTTACTGGAAG
  ACCAGGTCCTGTGGGGGGTCCTGGTTCAGCTGGAGCCAAAGGTGAGAGTGGGGATCCAGGTCCTCAGGGT
  CCTCGAGGTGTCCAAGGTCCTCCTGGTCCAACGGGAAAGCCTGGAAAGAGGGGCCGTCCAGGTGCTGATG
  GAGGAAGAGGCATGCCAGGAGAATCTGGGTCTAAGGGAGACCGAGGGTTTGATGGACTTCCAGGTCTGCC
  TGGTGACAAAGGTCACAGGGGAGAAAGAGGTCCACAAGGTCCACCTGGCCTCCCTGGCGATGATGGAATG
  AGGGGAGAAGATGGGGAGATCGGGCCCAGGGGTCTTCCAGGTGAAGCTGGTCCAAGAGGCTTGCTGGGAC
  CAAGGGGAACTCCAGGACCCCCTGGGCAGCCTGGTATCGGAGGTATAGATGGCCCCCAAGGACCAAAAGG
  AAACATGGGTCCCCAAGGGGAGCCTGGACCACCAGGTCAGCAAGGAAATCCAGGACCTCAAGGACTTCCT
  GGTCCACAAGGCCCAATTGGCCCTCCAGGAGAAAAAGGGCCACAAGGAAAACCAGGGCTTGCGGGACTTC
  CTGGTGCTGATGGACCTCCTGGTCATCCTGGGAAAGAAGGCCAGTCTGGAGAAAAGGGTGCCCTGGGTCC
  TCCTGGTCCTCAGGGGCCTATTGGTTATCCTGGTCCCCGTGGAGTAAAGGGAGCAGATGGTGTCAGAGGT
  CTCAAGGGCTCTAAAGGCGAAAAGGGTGAAGATGGCTTTCCAGGATTCAAAGGTGACATGGGTCTTAAAG
  GTGACAGAGGCGAGGTGGGTCAAGTTGGCCCAAGAGGAGAAGATGGCCCTGAAGGCCCCAAAGGCCGTGC
  AGGCCCAACTGGAGACCCAGGTCCCTCTGGCCAAGCAGGAGAGAAGGGAAAACTTGGGGTTCCAGGATTG
  CCGGGATATCCAGGAAGACAAGGTCCAAAGGGATCCACTGGATTTCCTGGATTTCCAGGTGCCAACGGGG
  AGAAAGGTGCCCGGGGAATTGCTGGCAAACCAGGCCCCCGGGGACAGCGTGGTCCAACGGGTCCTCGAGG
  TTCCAGAGGCGCCAGAGGTCCCACGGGAAAACCTGGTCCGAAGGGTACTTCAGGTGGTGATGGCCCTCCT
  GGTCCTCCAGGCGAAAGGGGCCCTCAAGGACCTCAAGGTCCAGTTGGATTCCCTGGACCAAAAGGCCCCC
  CTGGCCCTGCTGGGAAAGATGGGCTACCAGGACACCCTGGGCAGCGTGGTGAGACTGGATTTCAAGGCAA
  GACAGGTCCCCCTGGACCAGGAGGTGTGGTAGGACCACAGGGACCAACTGGTGAGACTGGTCCTATAGGT
  GAACGTGGCCATCCCGGCCCTCCTGGTCCTCCTGGCGAGCAAGGACTTCCCGGTGCTGCAGGGAAAGAAG
  GTGCAAAGGGTGATCCCGGGCCTCAAGGTATCTCAGGAAAAGATGGACCAGCAGGAATACGCGGTTTCCC
  AGGTGAAAGAGGCCTTCCTGGAGCCCAGGGTGCACCTGGGCTGAAAGGAGGAGAAGGTCCCCAGGGCCCA
  CAGGGTCCAGTTGGCTCTCCTGGAGAGCGAGGCTCAGCAGGCACAGCAGGCCCAATTGGTTTGCCAGGAC
  GTCCAGGCCCCCAGGGTCCTCCTGGTCCAGCTGGAGAAAAAGGTGCTCCTGGAGAAAAGGGTCCCCAGGG
  TCCTGCAGGTAGAGATGGTGTCCAAGGCCCTGTGGGTCTCCCAGGACCTGCTGGTCCTGCTGGCTCTCCT
  GGAGAAGATGGAGACAAGGGTGAAATTGGTGAACCAGGCCAAAAGGGCAGCAAAGGTGACAAAGGCGAAA
  ATGGTCCTCCTGGTCCCCCCGGACTTCAAGGACCTGTTGGTGCTCCTGGAATCGCTGGAGGCGATGGTGA
  GCCAGGTCCCCGAGGACAGCAGGGAATGTTTGGACAAAAAGGTGATGAGGGCGCACGAGGTTTCCCAGGA
  CTTCCTGGCCCCATAGGTCTTCAGGGGTTGCCAGGCCCACCAGGTGAAAAGGGCGAAAATGGAGACGTTG
  GCCCCATGGGTCCACCAGGTCCTCCTGGCCCACGAGGCCCTCAAGGTCCCAATGGAGCTGATGGACCACA
  AGGACCCCCAGGCTCCATTGGTTCAGTTGGTGTTGTGGGAGACAAGGGTGAACCTGGAGAAGCAGGGAAC
  CCAGGGCCCCCTGGGGAAGCTGGCTCTGGTGGTCTCAAAGGAGAGAGAGGAGAGAAAGGAGAAGCTGGGC
  CCCCTGGTGCTGCAGGTCCTGCTGGTATTAAGGGGCCACCAGGTGATGATGGGCCCAAGGGGAACCCGGG
  ACCCGTTGGGTTTCCTGGAGATCCTGGTCCACCTGGAGAACCTGGTCCTGCAGGGCAAGATGGTGTTGGA
  GGTGACAAGGGTGAAGATGGAGATCCAGGACAACCAGGTCCCCCTGGTCCATCTGGTGAAGCTGGCCCTC
  CAGGTCCTCCTGGGAAGAGAGGTCCGCCTGGAGCTTCAGGTTCAGAAGGAAGGCAAGGAGAAAAAGGTGC
  TAAGGGAGAAGCTGGTGCTGAAGGACCTCCTGGGAAAACTGGCCCTGTCGGACCTCAGGGACCTTCTGGA
  AAGCCTGGTCCAGAAGGTCTTCGAGGCATCCCTGGTCCTGTGGGAGAACAAGGTCTCCCAGGTGCTGCTG
  GCCAAGATGGACCTCCTGGTCCTCTGGGACCTCCTGGTTTACCTGGTCTCAAAGGTGACCCTGGATCCAA
  GGGTGAAAAGGGACATCCTGGTTTAATTGGATTGATTGGTCCTCCAGGAGAACAAGGGGAAAAAGGTGAC
  AGAGGACTTCCTGGGACTCAAGGTTCTCCAGGAGCCAAAGGCGATGGGGGCATCCCAGGTCCTGCTGGTC
  CCATAGGTCCCCCTGGTCCTCCAGGATTACCAGGTCCCGCTGGCCCGAAGGGTAACAAAGGATCATCTGG
  ACCTACTGGCCAGAAGGGTGACAGTGGTATGCCAGGGCCTCCCGGGCCTCCAGGTCCTCCTGGAGAAGTG
  ATACAGCCTTTGCCTATTTTGTCACCAAAAAAGACCAGAAGACACACTGAAAGCATCCAGGGTGATGCAG
  GAGATAATATTCTTGACTACTCAGATGGCATGGAGGAGATATTTGGTTCCCTCAATTCTCTGAAACAAGA
  CATTGAACACATGAAGTTTCCCATGGGCACACAGACCAACCCAGCACGAACATGCAAAGACCTGCAACTC
  AGCCATCCCGACTTCCCAGATGGTGAATATTGGATTGATCCTAACCAAGGTTGTTCAGGTGATTCCTTCA
  AAGTTTACTGTAATTTCACAGCTGGTGGTGAGACATGCATCTATCCGGATAAAAAATCTGAGGGAGTAAG
  AATTTCATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGAGAGGAAAACTGCTTTCA
  TATTTAGATGTGGAAGGCAATTCCATAAATATGGTACAAATGACATTCCTGAAGCTCCTGACTGCCTCTG
  CCCGGCAAAACTTCACCTACAATTGCCATCAGTCAGCTGCCTGGTATGACGTATTATCAGGAAGTTATGA
  CAAAGCACTTCGATTTCTGGGATCAAATGATGAGGAAATGTCCTATGAGAACAACCCACACATCAAAGCT
  TTGTATGATGGCTGTGCGTCTCGAAAAGGCTATGAAAAGACAGTGATTGAGATCAATACTCCGAAAATTG
  ATCAGGTACCCATCATTGATGTAATGATCAATGATTTTGGTGATCAGAACCAGAAGTTTGGATTTGAAGT
  CGGTCCAGCTTGCTTTCTTGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212085 representing NM_007729
  Red=Cloning site Green=Tags(s)
  
  MEPWSRWKTKRWIWDLTISTLALTFLFQAREVRGAAPVDILKALDFHNSPVGISKTTGFCTNRKNSKDPD
  VAYRVTEEAQISAPTKQLFPGGIFPQDFSILFTIKPKKGTQAFLLSLYNEHGIQQLGVEVGRSPVFLFED
  HTGKPTPENYPLFSTVNIADGKWHRVAISVEKKTVTMIVDCKKKITKPLDRSERSIVDTNGIMVFGTRIL
  ETDVFQGDIQQFLITGDPKAAYDYCDHYSPDCDLTSKAAQAQEPHIDEYAPEDIIEYDYEYGETDYKEAE
  SVTEMPTFTEETVAQTEANIVDDFQDYNYGTMEPYQTETPRRVSGSNEPNPVEEGFTEEYLTGEDYDVQR
  NTSEDILYGNKGVDGRDSDLLVDGDLGEYDFYEYKEYEERTTTSPNEEFGPGVPAETDFTETSINGHGAY
  GEKGQKGEPAVVEPGMLVEGPPGPAGPAGLMGPPGLQGPSGLPGDPGDRGPPGRPGLPGADGLPGPPGTM
  LMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSAGAKGESGDPGPQG
  PRGVQGPPGPTGKPGKRGRPGADGGRGMPGESGSKGDRGFDGLPGLPGDKGHRGERGPQGPPGLPGDDGM
  RGEDGEIGPRGLPGEAGPRGLLGPRGTPGPPGQPGIGGIDGPQGPKGNMGPQGEPGPPGQQGNPGPQGLP
  GPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRG
  LKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGL
  PGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPP
  GPPGERGPQGPQGPVGFPGPKGPPGPAGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIG
  ERGHPGPPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGIRGFPGERGLPGAQGAPGLKGGEGPQGP
  QGPVGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSP
  GEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEPGPRGQQGMFGQKGDEGARGFPG
  LPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSIGSVGVVGDKGEPGEAGN
  PGPPGEAGSGGLKGERGEKGEAGPPGAAGPAGIKGPPGDDGPKGNPGPVGFPGDPGPPGEPGPAGQDGVG
  GDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGASGSEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPSG
  KPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPLGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGD
  RGLPGTQGSPGAKGDGGIPGPAGPIGPPGPPGLPGPAGPKGNKGSSGPTGQKGDSGMPGPPGPPGPPGEV
  IQPLPILSPKKTRRHTESIQGDAGDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQL
  SHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRISSWPKEKPGSWYSEFKRGKLLS
  YLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSAAWYDVLSGSYDKALRFLGSNDEEMSYENNPHIKA
  LYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQNQKFGFEVGPACFLG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007729
• ORF Size: 5412 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007729.2
• RefSeq Size: 7275
• RefSeq ORF: 5415
• Locus ID: 12814
• Gene Summary: This gene encodes the alpha-1 subunit of type XI collagen, one of the low abundance fibrillar collagens that is essential for normal embryonic skeletal development and the cohesive properties of cartilage. The encoded protein, in association with the alpha-1 subunit of type II collagen, forms a heterotrimeric type XI procollagen that undergoes proteolytic processing. Mice lacking the encoded protein develop severe chondrodysplasia and die at birth. [provided by RefSeq, Dec 2015]