Mylk (NM_139300) Mouse Tagged ORF Clone

CAT#: MG212102

Mylk (GFP-tagged) - Mouse myosin, light polypeptide kinase (Mylk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mylk
• Synonyms: 9530072E15Rik; A930019C19Rik; AW489456; KRP; Mlck; MLCK108; MLCK210; nmMlck; smMLCK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212102 representing NM_139300
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGCCAGTTACCTGGGAAGACCATGGGGGACGTGAAACTGTTTGCCTCGTCACACATGTCTAAAA
  CCTCCCACAGTGTGGATCCCTCGAAGGTCAGCTCCATGCCTCTCACCGAGGCCCCAGCTTTCATTCTGCC
  CCCTCGGAACCTCTGCGTCAAAGAAGGAGCCACAGCCAAGTTCGAAGGGAGGGTCCGGGGCTACCCAGAG
  CCCCAGGTGACATGGCACAGAAAAGGGCAAGCCATCACCAATGGTGGCCGCTTCCTGCTGGACTGTGGAG
  TGCGGGGGACTTTTAGCCTTGTCATCCACACTGTCCGCGAGGAGGACAAGGGCAAATACACCTGTGAAGC
  TAGCAATGGCAGTGGCGCCCGCCAGGTGACCGTGGAGCTGACTGTGGAAGGGAATTCTATGAAGAAGCGT
  GATCAGCCTGTTCTTTCTAAAGCCTCAGGATTTCCAGGGGAGACTCGTCCTAGCATCTGGGGTGAGTGCC
  CACCAAAGTTTGCCACCAAGCTGGGCAGAGCTGTGGTCAAAGAAGGGCAGATGTGGCGATTCTCCTGCAA
  GATCACCGGCCGGCCTCCACCACAGGTCACCTGGCTCAAGGGAAATGTCCCGTTGCAGCCAAGTGCCCGC
  GTGTCAATGTCAGAGAAGAATGGCATGCAGATCCTGGAGATCCGTGGGGTCACTCGGGATGACCTGGGCG
  TGTACACCTGCATGGTGGTGAATGGGTCAGGGAAAGCCTCCATGTCGGCGGAGCTCTCCATCCCAGGTTT
  GGACAATGCCTCTAGGTTGGCTGTGAGAGGAACCAAAGCCCCCAGTCCAGACATCAGGAAGGAGGTGACC
  AATGGAGTCTCCAAAGACCCAGAGACTGTGGCTGAAAGTAAGAACTGTCCAAGTCCCCAGAGGAGTGGCT
  CCTCAGCCCGGGCCACCAACAGTCACCTCAAGTCCCCGCAGGAGCCCAAGCCAAAGCTGTGTGAGGATGC
  TCCCAGAAAGGTCCCGCAGTCCTCCATCCTGCAGAAGTCCACCAGCACCATCACTCTGCAGGCCTTGAAA
  GTCCAGCCAGAAGCGAGAGTACCAGCCATAGGGTCCTTCTCTCCTGGGGAAGACAGGAAGAGTCTGGCTG
  CCCCTCAGCAAGCCACACTCCCTACCAGACAGTCTAGCCTGGGAGGTTCCGTTGGGAACAAGTTTGTTAC
  CGGGAACATCCCCAGAGAGAGCCAAAGGGAGTCAACATTCCCCAGATTTGAGAGTCAGCCCCAAAGCCAG
  GAGGTCACTGAAGGTCAGACAGTCAAGTTCATCTGCGAGGTTTCAGGTATACCAAAGCCTGACGTGGGCT
  GGTTCCTGGAAGGCATCCCCGTGAGGAGACGGGAAGGCATCACTGAGGTTTATGAAGATGGTGTATCCCA
  TCACCTGTGCCTGCTGAGAGCCCGGACGAGAGACAGTAGGAAGTACAGCTGCACAGCATCCAATAGCCTT
  GGCCAGGTGTCCTGCAGCTGGAGCCTGCTGGTGGACCGGCCGAACTTGGCACAGACAGCCCCTTCATTCT
  CTAGTGTTCTGAAGGACAGTGTTGTCATTGAGGGCCAGGATTTTGTGCTACGGTGCTCAGTGCAGGGGAC
  TCCAGCACCCCGGGTCACGTGGCTGCTCAATGGACAGCCCATCCAGTTTGCTCACTCCATCTGTGAGGCA
  GGCGTGGCCGAGCTCCACATCCAGGATGCCCTGCCAGAGGACCGTGGCACCTACACCTGCCTGGCTGAGA
  ATGCCATGGGACAGGTGTCCTGCAGCGCCACTGTCACCGTCCAAGAGAAGAAAGGTGAAGGAGAGAGGGA
  ACACCGTCTGTCCCCAGCTCGCAGCAAGCCCATCGCACCCATCTTCCTGCAGGGCCTCTCCGACCTCAAA
  GTCATGGATGGAAGCCAGGTCACTATGACAGTCCAGGTGTCAGGGAACCCACCTCCGGAAGTCATCTGGC
  TTCACGACGGGAATGAGATTCAGGAGTCCGAGGACTTCCACTTCGAGCAGAAGGGTGGCTGGCACAGCCT
  GTGTATCCAGGAGGTGTTCCCGGAGGACACGGGCACGTACACCTGTGAGGCCTGGAACAGTGCCGGGGAG
  GTCCGCACCCGGGCCGTCCTCACCGTGCAAGAGCCTCATGATGGCACCCAGCCCTGGTTCATCAGCAAGC
  CTCGTTCGGTGACAGCCACCCTGGGCCAGAGTGTCCTCATCTCCTGCGCCATAGCTGGAGACCCCTTTCC
  CACCGTGCACTGGCTCCGGGATGGCAGAGCCCTCTCCAAGGACAGTGGCCACTTTGAGCTGCTCCAGAAT
  GAGGACGTGTTCACCCTGGTTCTAAAGAACGTGCAGCCCTGGCATGCCGGCCAGTATGAGATTCTGCTCA
  AGAACCGGGTTGGCGAGTGTAGTTGCCAAGTGTCACTGATGCTTCACAACAGCCCTTCCAGAGCCCCACC
  ACGGGGGCGGGAGCCTGCCAGCTGCGAGGGCCTCTGTGGTGGTGGAGGAGTTGGTGCTCATGGTGATGGT
  GACCGTCATGGGACCCTGAGGCCCTGCTGGCCAGCAAGAGGGCAAGGTTGGCCTGAGGAGGAAGACGGAG
  AAGACGTGAGGGGGCTGTTGAAGAGACGGGTGGAGACCAGGCTGCACACAGAAGAGGCCATCCGCCAGCA
  GGAGGTGGGGCAGCTGGATTTTCGAGACCTCCTGGGAAAGAAGGTGAGCACCAAGACCGTCTCAGAAGAC
  GACCTGAAGGACATCCCAGCCGAGCAGATGGATTTCCGCGCCAACCTTCAGCGGCAAGTGAAGCCAAAGA
  CCATATCCGAGGAAGAGAGGAAGGTGCATAGCCCTCAGCAGGTTGACTTCCGGTCTGTCCTCGCCAAGAA
  GGGGACCCCTAAGACCCCCGTTCCTGAGAAGGCACCTCCAAAAGCTGCCACCCCAGACTTTCGCTCTGTG
  CTAGGTGGCAAGAAGAAGTCACCCTCGGAGAATGGGGGCAACAGCGCTGAGGTCTTGAATGTCAAGGCTG
  GGGAAAGCCCCACACCTGCAGGCGATGCGCAGGCCATCGGGGCCCTGAAACCCGTAGGCAATGCCAAGCC
  TGCTGAGACCCCGAAACCTATAGGTAATGCCAAGCCTACCGAGACCCTGAAGCCTGTGGGCAACACCAAG
  CCTGCCGAGACCCTGAAACCCATAGCCAACGCACAGCCTTCAGGGTCCCTGAAACCTGTAACCAACGCAC
  AACCTGCTGAGCCCCAGAAGCCTGTGGGCAATGCCAAGTCTGCTGAGACCTCGAAACCAGCTGGGAAAGA
  AGAAGTCAAGGAGGTAAAGAATGATGTGAACTGTAAGAAAGGGCAGGTCGGAGCCACGGGCAATGAAAAG
  CGACCCGAGAGCCAAGGCTCGGCCCCAGTTTTCAAGGAAAAGCTACAAGATGTCCATGTGGCAGAGGGGG
  AGAAGCTGCTACTCCAATGCCAGGTGATCTCTGATCCCCCCGCCACTGTCACCTGGTCGCTGAATGGGAA
  GACACTCAAGACCACAAAATTCATCGTCCTCGCCCAAGAAGGCTCACGTTTCTCTGTCTCCATCGAGAAG
  GCACTGCCTGAGGACCGAGGTTTGTACAAGTGTGTAGCCAAGAACAGCGCTGGCCAGGCAGAGTGTTCCT
  GCCAAGTCACTGTGGACGATGCTCAGACCAGTGAGAACACCAAGGCCCCGGAGATGAAATCCCGGAGACC
  CAAGAGCTCTCTTCCCCCTGTGCTAGGAACAGAGAGCGACGCAACTGTGAAAAAGAAACCTGCCCCCAAG
  ACACCCACAAAAGCAGCTATGCCCCCTCAAATCATCCAGTTCCCCGAGGACCAGAAGGTGCGCGCAGGAG
  AACCTGTGGAGCTGTTTGGGAAAGTGGCTGGCACCCAGCCCATCACCTGCAAGTGGATGAAGTTCCGAAA
  GCAGATCCAGGAGAGCGAGCATATCAAGGTGGAGAATGGCGAGAGCGGCAGCAAGCTCACCATCCTGGCC
  GCGCGGCAGGAGCACTGTGGCTGCTACACACTGGTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAAG
  TCAACCTCACGGTCGTGGATAAGCCAGACCCCCCGGCTGGCACACCTTGTGCTTCTGACATTCGGAGTTC
  CTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAATGTGGAG
  ATCTGGGACACAGAGGACAAGGTGTGGAAAGAGCTAGCCACGTGCCGCAGCACCTCTTTTAATGTCCAAG
  ACCTGCTGCCTGACCGCGAGTACAAATTCCGAGTGCGTGCAGTCAACGTCTATGGAACCAGCGAGCCAAG
  CCAAGAGTCCGAGCTCACAGCTGTGGGAGAGAAACCTGAAGAGCCAAAGGATGAAGTGGAAGTGTCCGAT
  GATGACGAAAAGGAACCTGAGGTTGATTACCGGACGGTGACTGTTAACACTGAACAGAAAGTGTCTGATG
  TGTATGACATTGAAGAGCGACTAGGATCTGGGAAGTTTGGACAAGTCTTCCGACTTGTGGAAAAGAAAAC
  TGGAAAAATCTGGGCAGGGAAATTTTTCAAGGCCTATTCCGCCAAGGAGAAAGACAACATCCGTCAGGAG
  ATCAGCATCATGAACTGCCTCCACCACCCCAAGCTGGTCCAGTGTGTGGATGCCTTCGAAGAAAAGGCCA
  ACATTGTCATGGTTCTGGAGATCGTGTCCGGGGGTGAGCTGTTTGAGCGTATCATTGACGAGGACTTCGA
  GCTGACGGAGCGGGAGTGTATCAAGTACATGAGGCAGATCTCGGAAGGGGTGGAGTACATCCACAAGCAG
  GGCATTGTGCACCTGGACCTCAAGCCCGAGAACATCATGTGTGTCAACAAGACAGGCACCAGGATCAAGC
  TCATTGACTTCGGCCTGGCACGGAGACTCGAGAACGCTGGGTCTCTGAAGGTCCTCTTTGGGACCCCAGA
  GTTTGTGGCTCCTGAAGTGATCAACCATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGTC
  ATCTGCTATATTCTGGTCAGCGGACTTTCTCCCTTCATGGGTGACAATGATAACGAGACCTTAGCCAACG
  TCACTTCAGCCACCTGGGACTTCGATGACGAGGCGTTCGATGAGATCTCCGATGACGCAAAGGATTTCAT
  CAGTAACTTGCTGAAGAAAGATATGAAAAACCGTCTGGACTGCACCCAGTGCCTTCAGCACCCATGGCTG
  ATGAAAGACACCAAGAACATGGAGGCCAAGAAGCTGTCCAAGGACCGGATGAAGAAATACATGGCAAGAA
  GGAAGTGGCAGAAAACGGGCAATGCTGTGAGAGCCATCGGGAGACTGTCCTCTATGGCAATGATCTCAGG
  GCTCAGTGGCAGGAAATCTTCAACAGGGTCACCAACCAGCCCAATCAATGCGGAGAAACTAGAGTCTGAA
  GACGATGTCTCCCAAGCTTTCCTGGAGGCTGTTGCTGAGGAAAAGCCCCATGTGAAACCTTATTTCTCTA
  AGACCATCCGTGATCTGGAGGTCGTGGAGGGAAGCGCTGCTAGATTTGACTGCAAGATTGAAGGATACCC
  AGACCCTGAGGTGGTCTGGTTCAAGGATGACCAATCAATCAGGGAGTCCCGCCACTTCCAGATAGACTAC
  GACGAAGACGGTAACTGCTCGCTCATCATCAGCGACGTCTGTGGGGATGATGACGCCAAGTACACCTGCA
  AGGCTGTCAACAGCCTCGGAGAAGCCACCTGCACTGCGGAGCTCATCGTGGAGACCATGGAGGAAGGGGA
  AGGGGAGGAGGGGGGAGAGGAGGAGGAGGAGGAGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212102 representing NM_139300
  Red=Cloning site Green=Tags(s)
  
  MSGQLPGKTMGDVKLFASSHMSKTSHSVDPSKVSSMPLTEAPAFILPPRNLCVKEGATAKFEGRVRGYPE
  PQVTWHRKGQAITNGGRFLLDCGVRGTFSLVIHTVREEDKGKYTCEASNGSGARQVTVELTVEGNSMKKR
  DQPVLSKASGFPGETRPSIWGECPPKFATKLGRAVVKEGQMWRFSCKITGRPPPQVTWLKGNVPLQPSAR
  VSMSEKNGMQILEIRGVTRDDLGVYTCMVVNGSGKASMSAELSIPGLDNASRLAVRGTKAPSPDIRKEVT
  NGVSKDPETVAESKNCPSPQRSGSSARATNSHLKSPQEPKPKLCEDAPRKVPQSSILQKSTSTITLQALK
  VQPEARVPAIGSFSPGEDRKSLAAPQQATLPTRQSSLGGSVGNKFVTGNIPRESQRESTFPRFESQPQSQ
  EVTEGQTVKFICEVSGIPKPDVGWFLEGIPVRRREGITEVYEDGVSHHLCLLRARTRDSRKYSCTASNSL
  GQVSCSWSLLVDRPNLAQTAPSFSSVLKDSVVIEGQDFVLRCSVQGTPAPRVTWLLNGQPIQFAHSICEA
  GVAELHIQDALPEDRGTYTCLAENAMGQVSCSATVTVQEKKGEGEREHRLSPARSKPIAPIFLQGLSDLK
  VMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGE
  VRTRAVLTVQEPHDGTQPWFISKPRSVTATLGQSVLISCAIAGDPFPTVHWLRDGRALSKDSGHFELLQN
  EDVFTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHNSPSRAPPRGREPASCEGLCGGGGVGAHGDG
  DRHGTLRPCWPARGQGWPEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSED
  DLKDIPAEQMDFRANLQRQVKPKTISEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKAPPKAATPDFRSV
  LGGKKKSPSENGGNSAEVLNVKAGESPTPAGDAQAIGALKPVGNAKPAETPKPIGNAKPTETLKPVGNTK
  PAETLKPIANAQPSGSLKPVTNAQPAEPQKPVGNAKSAETSKPAGKEEVKEVKNDVNCKKGQVGATGNEK
  RPESQGSAPVFKEKLQDVHVAEGEKLLLQCQVISDPPATVTWSLNGKTLKTTKFIVLAQEGSRFSVSIEK
  ALPEDRGLYKCVAKNSAGQAECSCQVTVDDAQTSENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPK
  TPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVAGTQPITCKWMKFRKQIQESEHIKVENGESGSKLTILA
  ARQEHCGCYTLVVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVE
  IWDTEDKVWKELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSD
  DDEKEPEVDYRTVTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKDNIRQE
  ISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQ
  GIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINHEPIGYATDMWSIGV
  ICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWL
  MKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESE
  DDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDY
  DEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEEEEE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139300
• ORF Size: 5850 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_139300.3, NP_647461.3
• RefSeq Size: 7809
• RefSeq ORF: 5853
• Locus ID: 107589