Pole (NM_011132) Mouse Tagged ORF Clone

CAT#: MG212119

Pole (GFP-tagged) - Mouse polymerase (DNA directed), epsilon (Pole)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pole
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212119 representing NM_011132
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTGAGGAACAGTGGACGGAGGCACCCCGAGCCGGGCGCGGATGGCGAAGGCAGCCGGGATGATG
  GTCCCTCTTCCTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACAGACAAGATGGACTTACG
  GTTTGGTTTCGAAAGGCTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTGATCAACATGCACCCTACTGAG
  ATCTTAGATGAAGACAAACGCTTAGTCAGCGCGGTGGATTACTACTTCATTCAAGATGATGGAAGCAGAT
  TTAAGGTGGCCTTGCCCTATATGCCGTATTTCTACATTGCAGCGAGAAAGGGTTGTGATCGAGAAGTTTC
  ATCTTTTCTATCCAAGAAGTTTCAGGGAAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGATCTGGAC
  TTGCCAAATCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGACCTTG
  TCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGATGAGTA
  TACAACAATGCTCTCCAGTATTCTGCAAGGTGGCAGTGTAATTACTGATGAGGACGAAACCTCTAAGAAG
  ATAGCTGACCAATTGGACAACATAGTGGACATGCGAGAGTATGATGTTCCCTACCACATTCGCCTCTCCA
  TTGACCTCAGAATCCATGTGGCCCACTGGTACAATGTTAGATTTCGAGGAAATGCTTTTCCTGTGGAAAT
  CACCCGACGAGATGATCTTGTTGAACGACCTGACCCTGTGGTTTTGGCATTTGACATCGAGACGACCAAA
  CTGCCTCTCAAATTCCCTGATGCTGAGACCGATCAGATCATGATGATCTCCTATATGATTGATGGCCAGG
  GCTACCTCATCACTAACAGGGAGATTGTTTCAGAAGATATTGAAGATTTTGAGTTCACCCCTAAGCCAGA
  ATATGAAGGGCCCTTTTGTGTCTTCAATGAACCCGACGAGGTCCATCTGATCCAGAGATGGTTTGAGCAT
  ATCCAGGAAACCAAACCTACCATTATGGTCACCTACAATGGGGATTTTTTTGACTGGCCATTTGTGGAGG
  CTAGGGCAGCAATTCATGGCCTCAGCATGTACCAGGAGATAGGCTTCCAGAAGGATAGCCAGGGGGAATA
  TAAGGCACCACAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGTGGGC
  AGTCATAATCTCAAGGCAGCTGCCAAGGCCAAACTTGGCTATGACCCTGTAGAGCTGGACCCTGAGGACA
  TGTGTCGTATGGCCACTGAACAGCCCCAGACTCTGGCCACTTACTCAGTGTCAGATGCTGTGGCTACTTA
  CTACCTGTACATGAAATACGTCCACCCCTTCATATTCGCCCTGTGCACCATTATTCCCATGGAACCTGAT
  GAGGTGCTGCGGAAGGGCTCCGGGACACTGTGTGAAGCCTTGCTGATGGTGCAAGCTTTCCATGCCAACA
  TTATCTTCCCCAATAAGCAAGAGCAGGAGTTCAACAAGCTGACAGATGATGGCCACATGCTAGATGCTGA
  GACCTACGTCGGGGGCCACGTGGAGGCACTAGAGTCTGGTGTCTTCAGAAGTGATATCCCCTGCCGGTTT
  AGGATGAATCCTGCAGCCTTTGATTTCCTGCTGCAACGAGTCGAGAAGACTATGCGCCACGCCATTGAAG
  AAGAAGAGAAGGTGCCTGTGGAACAAGCCACCAACTTTCAAGAGGTGTGTGAGCAGATTAAGACCAAGCT
  CACCTCCCTAAAAGATGTTCCTAACAGAATTGAATGTCCTCTAATCTACCATCTAGATGTGGGGGCCATG
  TATCCTAACATAATTCTTACCAACCGCCTACAGCCTTCTGCCATAGTGGATGAGGCCACCTGTGCTGCCT
  GTGACTTCAATAAGCCTGGAGCAAGTTGTCAGAGGAAGATGGCCTGGCAGTGGAGGGGAGAATTCATGCC
  AGCCAGTCGCAGTGAATACCATCGGATTCAGCATCAGCTGGAGTCGGAGAAGTTTCCCCCTTTGTTTCCA
  GAGGGGCCAGCACGGGCCTTTCACGAGCTGTCCCGTGAAGAACAGGCTAAATACGAGAAGAGGAGGCTGG
  CAGATTATTGCCGGAAAGCCTATAAGAAGATCCATGTGACCAAGGTAGAAGAACGTCTAACTACCATCTG
  CCAGCGGGAAAACTCATTTTATGTGGACACAGTGCGGGCCTTCAGAGACAGGCGCTATGAGTTCAAAGGA
  CTGCACAAGGTGTGGAAGAAGAAGCTCTCGGCAGCTGTAGAGGTGGGCGATGCATCAGAGGTGAAGCGCT
  GCAAGAACATGGAGATCCTTTACGATTCACTGCAGCTGGCTCACAAGTGCATCCTGAACTCCTTCTACGG
  CTATGTCATGCGCAAAGGAGCTCGCTGGTATTCCATGGAGATGGCTGGTATCGTCTGCTTTACAGGAGCC
  AACATCATCACCCAAGCAAGAGAACTGATTGAGCAGATCGGGAGGCCTTTAGAATTGGACACGGACGGAA
  TATGGTGCGTCCTACCCAATAGCTTTCCTGAAAATTTTGTCATCAAGACAACCAATGCGAAGAAACCCAA
  ACTGACCATCTCCTATCCTGGTGCCATGTTGAACATCATGGTCAAGGAAGGCTTTACCAACCACCAGTAC
  CAGGAACTAACAGAGCCTTCGTCTCTCACCTATGTCACCCACTCTGAGAATAGTATCTTTTTTGAAGTCG
  ATGGACCATACCTTGCTATGATCCTTCCAGCCTCCAAGGAAGAAGGCAAGAAGCTGAAGAAAAGATATGC
  TGTGTTCAATGAAGATGGTTCCTTGGCTGAACTGAAAGGTTTTGAGGTGAAACGCCGAGGGGAGTTGCAG
  CTGATTAAAATATTCCAGTCCTCAGTTTTTGAGGCCTTCCTCAAGGGCAGCACACTGGAGGAAGTGTATG
  GCTCGGTGGCCAAAGTGGCTGACTACTGGCTAGATGTGCTCTATAGCAAGGCTGCTAATATGCCCGATTC
  TGAATTGTTTGAGCTGATTTCTGAGAACCGCTCCATGTCTCGGAAGCTGGAAGATTACGGGGAGCAGAAG
  TCTACATCCATCAGCACAGCAAAGCGCCTGGCTGAGTTCCTGGGAGACCAGATGGTCAAAGATGCTGGAC
  TGAGCTGCCGCTATATCATCTCCCGAAAGCCAGAGGGGTCTCCTGTCACTGAGAGGGCCATTCCACTTGC
  CATTTTCCAAGCAGAGCCTACAGTGAGGAAACATTTTCTCCGGAAATGGCTAAAGAGTTCATCACTTCAA
  GACTTTGATATTCGGACAATTCTGGACTGGGACTACTACATAGAGAGGCTGGGGAGTGCCATCCAGAAAA
  TCATCACCATCCCCGCAGCTCTGCAGCAGGTGAAGAACCCAGTTCCACGTGTCAAACATCCAGACTGGCT
  ACACAAAAAACTACTAGAGAAGAATGATATCTACAAACAGAAGAAGATCAGTGAGCTCTTTGTGCTTGAA
  GGAAAGAGACAGATTGTGATGGCCCAGGCTTCAGAAAACAGTCTGAGTCTCTGCACTCCAGACATGGAGG
  ACATTGGACTCACAAAGCCACACCACTCTACAGTCCCAGTTGCTACTAAGAGGAAGCGAGTCTGGGAGAC
  CCAAAAGGAGTCTCAGGATATTGCACTAACTGTGCCCTGGCAAGAGGTCTTAGGGCAGCCTCCCTCCCTT
  GGAACCACACAGGAAGAGTGGTTGGTCTGGCTCCAGTTCCACAAGAAAAAGTGGCAGCTGCAGGCCCAAC
  AGCGCCTAGCCCTCAGGAAGAAGCAACGCTTAGAGTCAGCAGAAGATATGCCAAGGCTTGGGCCTATCCG
  AGAGGGGCCTTCCACAGGACTGGGGAGCTTTTTGCGAAGGACTGCCCGCAGCATCATGGACCTTCCATGG
  CAGATAATACAGATCAGTGAGACCAGACAGGCTGGTCTGTTCCGGCTGTGGGCTATCATTGGCAATGACT
  TGCACTGCATCAAGCTGAGTATCCCTCGAGTATTCTATGTAAACCAGCGGGTTGCCAAAGCAGAGGATGG
  ACCTGCATATCGGAAGGTGAATCGGGCTCTTCCTCGTTCCAACATTGTCTACAATCTCTATGAGTATTCA
  GTACCAGAGGACATGTACCAAGAACACATCAACGAGATCAACACTGAGTTGTCAGTACCAGACATTGAGG
  GCGTGTATGAGACACAGGTCCCATTGTTATTCCGGGCCCTCGTGCAGCTGGGCTGTGTGTGTGTGGTCAA
  CAAGCAGCTGACAAGGCACCTTTCGGGCTGGGAAGCTGAAACTTTTGCCCTCGAGCACCTTGAAATGCGT
  TCTCTGGCCCAGTTCAGCTACTTGGAACCAGGGAGTATCCGCCATATCTACCTGTACCATCACACTCAGG
  GCCACAAGGCACTCTTTGGGGTCTTTATCCCCTCACAGCGAAGAGCATCTGTGTTTGTGTTGGATACTGT
  ACGAAGCAACCAAATGCCAGGGCTCAGTGCCCTGTACTCATCAGAACACAGCCTGCTGCTGGACAAGGTG
  GACCCCAAGCTCCTGCCTCCCCCAAAACACACCTTTGAAGTTCGTGCTGAAACCAACCTGAAGACTATCT
  GCAGAGCCATCCAGCGCTTCCTGCTTGCCTACAAGGAAGAGCGCCGAGGGCCCACACTCATCGCTGTCCA
  GTCTAGCTGGGAGCTGTGTAGGCTGACCAGTGAGATTCCAGTCTTAGAAGAGTTCCCACTAGTGCCTATC
  CGAGTGGCTGACAAGATCAGCTATGCAGTCCTGGACTGGCAGCGCCATGGAGCTCGCCGAATGATCCGGC
  ACTACCTCAATTTAGACTTGTGCCTGTCGCAGGCCTTTGAGATGAGCAGGTACTTCCACATCCCTGTTGG
  AAACCTGCCGGAAGACATCTCCACCTTTGGCTCAGACCTCTTTTTTGCACGCCACCTCCAGCACCATAAC
  CACCTGCTTTGGCTATCCCCTACCTCTCGGCCTGACCTGGGTGGGAAGGAAGCTGATGACAACCGCCTTG
  TCATGGAGTTTGATGACCGAGCCACTGTGGAGATCAATAGTTCTGGCTGTTACTCTACTGTGTGCGTGGA
  ACTGGACATTCAAAATCTGGCAGTCAACACCATCCTCCAGTCCCATCATGTCAATGACATGGAGGGGGCT
  GGCAGCATGGGCATCAGCTTCGATGTGATCCAGCAGGCCTCCCTAGAGGACATGGTAACAGGCAATCAAG
  CTGCCAGTGCCCTGGCCAACTACGATGAGACAGCCCTCTGCTCTAGTACCTTCAGGATCCTGAAGAGCAT
  GGTGGTTGGCTGGGTAAAGGAAATCACACAGTACCACAACATCTATGCTGACAACCAGGTAATGCACTTC
  TACCGCTGGCTCCAGTCACCGTGCTCTCTGCTCCACGACCCAGCCCTTCACCGGACGCTGCACAACATGA
  TGAAGAAGCTCTTCCTGCAGCTCATTGCTGAGTTCAAGCGCCTGGGGTCATCAGTCGTCTATGCCAACTT
  CAATCGCATCATTCTCTGTACAAAGAAGCGCCGAATAGAGGATGCCCTTGCCTATGTGGAATATATTACC
  AACAGCATCCATTCTAAAGAGATCTTCCATTCCCTGACCATCTCTTTCTCTCGATGCTGGGAATTCCTTC
  TCTGGATGGATCCATCCAACTATGGTGGAATCAAAGGAAAAGTTCCATCTAGTATTCACTGTGGACAGGT
  AAAAGAGCAAGACTCCCAGGCAAGAGAGGAAACTGATGAAGAGGAGGAGGACAAGGAAAAGGACGAGGAG
  GAAGAGGGCATGGGAGAGTCCGAGGTTGAGGACTTACTGGAGAACAACTGGAACATTCTACAGTTCTTGC
  CCCAGGCAGCCTCTTGCCAGAGCTACTTCCTCATGATTGTTTCAGCATACATCGTAGCTGTGTACCAAAG
  CATGAAGGAGGAGTTGAGACACAGTGCCCCGGGCAGTACCCCTGTGAAGAGGAAGGGGGCCAGCCAGTTC
  TCCCAGGAGTCTGAAGGGGCAACTGGATCTCTTCCTGGAATGATCACTTTCTCTCAAGATTATGTGGCAA
  ATGAGCTCACTCAGAGCTTCTTCACCATTACTCAGAAAATTCAGAAGAAAGTCACAGGTTCTCGGAACAC
  CACTGAGCCCTCAGAGATGTTCCCCGTCCTCCCTGGTTCACACTTGCTGCTCAATAATCCTGCTCTGGAG
  TTCATCAAATATGTGTGCAAGGTACTATCTCTGGATACAAACATCACAAATCAGGTGAATAAGCTGAACA
  GAGACCTTCTTCGCCTGGTAGACGTTGGTGAATTCTCTGAGGAGGCCCAGTTCAGAGACCCCTGCCACTC
  CTACGTGCTCCCTGAGGTAATCTGCCACAGCTGTAATTTCTGCCGAGACCTGGACCTGTGCAAAGATTCC
  TCTTTCTCTCAGGATGGAGCCATCCTGCCTCAGTGGCTCTGCTCCAATTGTCAAGCCCCCTATGACTCCT
  CTGCCATTGAGTCAGCCTTGGTGGAAGCCCTGCAGAGGAAACTGATGGCCTTCACACTTCAGGACCTGGT
  ATGCCTCAAGTGCCGTGGTATGAAAGAGACCCATATGCCTGTGTACTGCAGCTGCGCAGGGGACTTTACT
  CTCACCATCCGCACTGAGGTCTTCATGGAACAGATTAGAATCTTCCAGAACATTGCCAAGTACTACAGCA
  TGTCATATCTCCAGGAGACCATAGAATGGCTGTTACAGACAAGCCCTGTATCAAACTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212119 representing NM_011132
  Red=Cloning site Green=Tags(s)
  
  MVLRNSGRRHPEPGADGEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINMHPTE
  ILDEDKRLVSAVDYYFIQDDGSRFKVALPYMPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLD
  LPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKK
  IADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEITRRDDLVERPDPVVLAFDIETTK
  LPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEH
  IQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVG
  SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPD
  EVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHMLDAETYVGGHVEALESGVFRSDIPCRF
  RMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAM
  YPNIILTNRLQPSAIVDEATCAACDFNKPGASCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFP
  EGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKG
  LHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGA
  NIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNAKKPKLTISYPGAMLNIMVKEGFTNHQY
  QELTEPSSLTYVTHSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQ
  LIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQK
  STSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQ
  DFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLE
  GKRQIVMAQASENSLSLCTPDMEDIGLTKPHHSTVPVATKRKRVWETQKESQDIALTVPWQEVLGQPPSL
  GTTQEEWLVWLQFHKKKWQLQAQQRLALRKKQRLESAEDMPRLGPIREGPSTGLGSFLRRTARSIMDLPW
  QIIQISETRQAGLFRLWAIIGNDLHCIKLSIPRVFYVNQRVAKAEDGPAYRKVNRALPRSNIVYNLYEYS
  VPEDMYQEHINEINTELSVPDIEGVYETQVPLLFRALVQLGCVCVVNKQLTRHLSGWEAETFALEHLEMR
  SLAQFSYLEPGSIRHIYLYHHTQGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSSEHSLLLDKV
  DPKLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELCRLTSEIPVLEEFPLVPI
  RVADKISYAVLDWQRHGARRMIRHYLNLDLCLSQAFEMSRYFHIPVGNLPEDISTFGSDLFFARHLQHHN
  HLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINSSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGA
  GSMGISFDVIQQASLEDMVTGNQAASALANYDETALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHF
  YRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDALAYVEYIT
  NSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVKEQDSQAREETDEEEEDKEKDEE
  EEGMGESEVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYQSMKEELRHSAPGSTPVKRKGASQF
  SQESEGATGSLPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNTTEPSEMFPVLPGSHLLLNNPALE
  FIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDS
  SFSQDGAILPQWLCSNCQAPYDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCSCAGDFT
  LTIRTEVFMEQIRIFQNIAKYYSMSYLQETIEWLLQTSPVSNC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011132
• ORF Size: 6849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011132.2, NP_035262.2
• RefSeq Size: 7167
• RefSeq ORF: 6852
• Locus ID: 18973
• Gene Summary: Participates in DNA repair and in chromosomal DNA replication. [UniProtKB/Swiss-Prot Function]