Gm5346 (NM_001025240) Mouse Tagged ORF Clone

CAT#: MG212507

Gm5346 (GFP-tagged) - Mouse predicted gene 5346 (Gm5346), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gm5346
• Synonyms: EG384813
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212507 representing NM_001025240
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGGGCTAAGGTCGTGGTCCATAAAAGGAACATGTTCCTTAAGTTCTGCCTGTGGACAATGTTCT
  TCTTCTCTGCATATTCACCAATTGGACATGCTAAGTACAGTAGCCTTCCAGAAGTAGTGACACCCTTGAG
  GGTCACTGTCACTAGAGGAAATAATAGTTCACCAGGTTGGCTGTCCTATAGCCTGAGCATTGGGGGGCAG
  AGGCACATAATCACTATGAAACCCAAGGAAAACTTGATATCCAGAAACTTCTTATTATTCACTTATAGTG
  ACCAAGGTGATCTCCTTGAAGAACATCCTTTTGTGCAGAATGACTGCTACTACCATGGCTATGTGGATGA
  GGACCCAGAGTCCCTGGTCATTGTCAACACCTGTTTTGGAAGTTTGCAAGGCACACTAGAAATAAATGGC
  ACAATTTATGAAATCATGCCCAGGAGCAAAACGTCAACATTTGAACATCTGGCTTATAAAATGGACAGTG
  GGGAGTCAGAACCATCTCCCATGAGATGTGGGTTATCAGAAGAGGAAATAGCACAACAAATGAACCTTCA
  GGAAAGCAATGCCTCCACTCTTTTGCAAATCCCCTATGAGAATTGGTGGACCCATCACAGGTTTATTGAT
  TATTTTGTAGTAATAGATCATAATTGCTATGTTCATAGAAATAACAATACCACGACTTGTATTCAAGATA
  TGTTGCAAGTAGTCAATGGAATAAATGGTTATTTTCTTCAAATACAGACTGATGTGGTTTTAACCAAAGT
  TGAAGTTTGGAGTCAAAATAATCTTATCAATGTAGAAGAAGAAATGTCAGCGGCCCTAGATGCATTCTGT
  AGTTGGAAGAAAAATGCGATTGGCAATCGTGTTAGACATGATATCATACACCTTTTTGTCAGACACAATT
  ATGTTAAATTTTTAGGTATGGCTAAATTAGGTCAAGTTTGTTTAACTCTTAACTGTGCAGTTAACAGTTT
  TCTTACTGATTCGTTATCATATCTGGCATTCATCGTAGCACATGAGATGGGTCATAACTTGGGCATGAAG
  CATGATGGAAGTGCATGTACATGTGGGTTACATACATGCATAATGTACCCATATGCATATATTTCTCCAA
  AATTCAGTAACTGTAGTTATGAAGAAATGTTTTCAGTTGTCAACAGAAGAAGTTGCTTGTACAATATTCC
  AGATGCACTAAAAACAATAACACTGGTGCCGACCATGTGTGGCAATAACTTGGTTGAAGAAGGAGAGCAG
  TGTGATTGTGGGAACTCTGAATCCTGTTTACAAGATCCTTGCTGTAGCAGCGACTGTGTTCTCAAACCTG
  GTGCTAAATGTGCTTTTGGACTGTGTTGCAAAGATTGCCAGTTCCTTCAAACAGGCACTGTGTGTAGACA
  AGAGAAAAATGAGTGTGACCTTCCAGAGTGGTGCAATGGAACTTCAGGTGAGTGTCCAGGAGATGTGTAT
  AAAGCAGATGGAATCCGTTGCAGTCGTGGTGGTTATTGCTATAAAATGGAATGTCAACGACATGATAGGC
  AGTGTCGGGAGATTTTTGGCAAGAGAAGTAGAAGTGCAGATGAAATTTGCTACATGGAAATGAACAGACG
  TGGTGACCGCTTTGGGAACTGTGGCAATGATAGCTCTATGTATAAAAGATGCGAGCTCACTGATGTACTC
  TGTGGGCGAATTCAGTGTGAGAATGTGATAAAACTTCCCCAAAGGAGAAACCATGAGACAGTGCATTATA
  CTCACTTCAGTAATAACACCTGCTGGACGATGGACTATCATTTTGGGATCACCATAGATGACGTTGGAGC
  AGTGAGCGATGGCACACCTTGTGCTCCAGACCATATATGTCTCGACAGAAAGTGTGTCAGCAAGTCTGTT
  CTGGTAAGTAACTGTACACCACACTTATGCCATATGCAAGGAGTCTGCAACAATAAACACCACTGCCATT
  GTACTAACACATGGGAACCACCGGACTGTCAACTACATGGCCATGGAGGTAGTATAGACAGTGGACCACC
  TCCAGTGCCTTTATCTCCTTCCAATTGGAGTATGTACTTCCTAGCTTTTATAATTATGTATGTGCTTGGC
  TTTATTGCTTTGTATGGCATTCGTGAATTAAGGAAAAAACTCCTAAAAGAATCGGTGCCAGAATCAGATT
  CTGGTGATAAAAATCTCACTAGAAAACAACAGGATCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212507 representing NM_001025240
  Red=Cloning site Green=Tags(s)
  
  MTGAKVVVHKRNMFLKFCLWTMFFFSAYSPIGHAKYSSLPEVVTPLRVTVTRGNNSSPGWLSYSLSIGGQ
  RHIITMKPKENLISRNFLLFTYSDQGDLLEEHPFVQNDCYYHGYVDEDPESLVIVNTCFGSLQGTLEING
  TIYEIMPRSKTSTFEHLAYKMDSGESEPSPMRCGLSEEEIAQQMNLQESNASTLLQIPYENWWTHHRFID
  YFVVIDHNCYVHRNNNTTTCIQDMLQVVNGINGYFLQIQTDVVLTKVEVWSQNNLINVEEEMSAALDAFC
  SWKKNAIGNRVRHDIIHLFVRHNYVKFLGMAKLGQVCLTLNCAVNSFLTDSLSYLAFIVAHEMGHNLGMK
  HDGSACTCGLHTCIMYPYAYISPKFSNCSYEEMFSVVNRRSCLYNIPDALKTITLVPTMCGNNLVEEGEQ
  CDCGNSESCLQDPCCSSDCVLKPGAKCAFGLCCKDCQFLQTGTVCRQEKNECDLPEWCNGTSGECPGDVY
  KADGIRCSRGGYCYKMECQRHDRQCREIFGKRSRSADEICYMEMNRRGDRFGNCGNDSSMYKRCELTDVL
  CGRIQCENVIKLPQRRNHETVHYTHFSNNTCWTMDYHFGITIDDVGAVSDGTPCAPDHICLDRKCVSKSV
  LVSNCTPHLCHMQGVCNNKHHCHCTNTWEPPDCQLHGHGGSIDSGPPPVPLSPSNWSMYFLAFIIMYVLG
  FIALYGIRELRKKLLKESVPESDSGDKNLTRKQQDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025240
• ORF Size: 2208 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025240.1, NP_001020411.1
• RefSeq Size: 2211 bp
• RefSeq ORF: 2211 bp
• Locus ID: 384813
• Cytogenetics: 8 A4