A4gnt (NM_001077424) Mouse Tagged ORF Clone

CAT#: MG215139

A4gnt (GFP-tagged) - Mouse alpha-14-N-acetylglucosaminyltransferase (A4gnt), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: A4gnt
• Synonyms: Alpha4gnt; AV080780; Gm798
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215139 representing NM_001077424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGGAAATTTATCTCTCCCTGTCCCTGGTCCTGGTGTTTGCCTGTGGCCTGCTGTACCAGCTCA
  CCATGAGGTCTCAGTGCTTCTTTGCCTGCCTGCCTCCTTTCTCATTTCCACAGGGACTGGACGGCCTCCT
  GAGGAGTGGACGAAGCATCATGTTTATAGAGACCTCTGAAAGGGTGGAGCCACCTCCGATGGTCTCCTGT
  GCTGTGGAGTCTGCTGCTAAAATCTATCCTGAGCAGCCCATCATCTTCTTCATGAAAGGACTCAGGGACT
  CCGTACAGCTGACTTCAAATACCAGCTACCCAGCCTTCTCCCTCCTCTCGGCCATTAATAATGTTTTCTT
  TGTGCCTTTGGACATGGAAAGACTGTTCAAAGACACACCCTTGTTTTCGTGGTACACAAAAGTCAACAGC
  AGTACAGAGAAACACTGGCTCCACGTCAGCTCCGACGCAGCCCGCCTGGCCATCATCTGGAAGTACGGAG
  GCATCTACATGGACACTGACGTCATCTCCCTTCAGCCCATCCCTGAGGAGAACTTCCTGGCAGCCCAGGG
  CTCCCGACACTCCAGTAACGGGGTATTTGGGTTCCTCCCCCACCACCCCTTCCTGTGGGCCTGCATGGAG
  AACTTTGTAGAGCACTATGACTCAACCATTTGGGGCAACCAAGGTCCCCAGTTGATGACAAGGATGTTAC
  GGGTGTGGTGTCGTCTTAAAGACTTCCACGGGTTGGGTGACCTGAAGTGTTTGAATATTTCTTTCCTTCA
  TCCCCAGAGATTTTACCCCATCCCTTATCCACAGTGGAAGCGCTACTACCAAGTGTGGGACAAAGAGCCC
  AGCTTCAATGAGTCCTACGCACTGCATTTGTGGAACTATATGAATAAAGAGGGCAAGACTGTGGTTAGAG
  GAAGCAAAACTCTGGTGGAAAATCTCTATCAAAAGCACTGTCCTAAGACTTATAGGGTTCTGATTCAAGG
  TGCAGAAGGGACAGTGTCCAAGAAGCCAGGCACAGGTTCCAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215139 representing NM_001077424
  Red=Cloning site Green=Tags(s)
  
  MLKEIYLSLSLVLVFACGLLYQLTMRSQCFFACLPPFSFPQGLDGLLRSGRSIMFIETSERVEPPPMVSC
  AVESAAKIYPEQPIIFFMKGLRDSVQLTSNTSYPAFSLLSAINNVFFVPLDMERLFKDTPLFSWYTKVNS
  STEKHWLHVSSDAARLAIIWKYGGIYMDTDVISLQPIPEENFLAAQGSRHSSNGVFGFLPHHPFLWACME
  NFVEHYDSTIWGNQGPQLMTRMLRVWCRLKDFHGLGDLKCLNISFLHPQRFYPIPYPQWKRYYQVWDKEP
  SFNESYALHLWNYMNKEGKTVVRGSKTLVENLYQKHCPKTYRVLIQGAEGTVSKKPGTGSR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077424
• ORF Size: 1023 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077424.2, NP_001070892.1
• RefSeq Size: 2757
• RefSeq ORF: 1026
• Locus ID: 333424
• Gene Summary: This gene encodes a protein from the glycosyltransferase 32 family. The encoded enzyme catalyzes the transfer of N-acetylglucosamine to alpha-1,4-linked beta-galactose residues. This enzyme is required for type III mucin synthesis and it is largely associated with the Golgi apparatus membrane. The encoded protein appears to be expressed in adenocarcinoma cells of pancreatic, biliary tract and gastric cancers. [provided by RefSeq, Jan 2010]