Abcc4 (NM_001033336) Mouse Tagged ORF Clone

CAT#: MG215338

Abcc4 (GFP-tagged) - Mouse ATP-binding cassette sub-family C (CFTR/MRP) member 4 (Abcc4) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abcc4
• Synonyms: ABCC4-N1; D630049P08Rik; MOATB; MRP4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215338 representing NM_001033336
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCGGTGCACACCGAGGTGAAACCCAACCCGCTGCAGGACGCCAACCTCTGCTCGCGCGTGTTCT
  TCTGGTGGCTCAACCCGCTGTTTAAAACTGGTCATAAGCGGAGACTGGAAGAAGATGACATGTTCTCAGT
  GCTTCCAGAAGATCGCTCAAAGCACCTCGGAGAGGAGCTTCAACGGTACTGGGATAAAGAACTTCTGCGA
  GCCAAGAAGGACTCGAGGAAGCCCTCCTTAACAAAGGCAATCATAAAGTGTTACTGGAAGTCTTACCTGA
  TTTTGGGAATTTTTACGTTAATTGAGGAGGGCACTCGAGTAGTTCAGCCCTTATTTTTAGGGAAAATTAT
  TGAATATTTTGAGAAGTATGACCCCGACGACTCGGTGGCTTTGCATACAGCTTATGGCTACGCAGCAGTG
  CTGTCCATGTGCACGCTCATCCTGGCCATACTACATCATTTGTACTTCTACCACGTGCAGTGCGCCGGGA
  TGAGGCTGCGGGTTGCCATGTGCCACATGATTTACCGGAAGGCACTCCGGTTAAGTAACTCGGCCATGGG
  GAAGACAACCACAGGCCAGATAGTTAACCTGCTGTCCAACGACGTGAACAAATTCGACCAAGTGACAATC
  TTCTTGCACTTTCTGTGGGCAGGGCCGCTGCAGGCCATCGCGGTAACCGTCCTCCTCTGGGTGGAGATAG
  GAATCTCCTGCCTGGCGGGCTTGGCCGTTCTGGTTATTCTTCTGCCTCTGCAAAGCTGCATCGGGAAGCT
  GTTCTCGTCACTGCGGAGTAAAACTGCGGCTTTCACGGATGCCAGGATCCGGACCATGAATGAAGTCATA
  ACAGGCATGAGGATAATAAAGATGTATGCGTGGGAGAAATCGTTTGCTGACCTCATTGCCAATCTGAGAA
  AGAAGGAGATTTCCAAGATTCTGGGCAGCTCCTACCTCAGAGGGATGAACATGGCGTCGTTTTTCATCGC
  AAACAAAGTCATCCTGTTCGTGACCTTCACTAGCTACGTGCTGCTTGGCAATGAGATTACAGCTAGCCAC
  GTGTTTGTGGCCATGACTCTGTACGGTGCCGTTCGGTTGACAGTCACCCTCTTCTTCCCGTCAGCCATTG
  AGAGAGGGTCAGAGGCCATCGTCAGCATTCGGAGGATCAAGAACTTTCTGTTACTCGATGAACTACCACA
  GCGCAAAGCCCATGTACCATCTGATGGCAAAGCCATTGTCCACGTGCAAGATTTCACCGCTTTCTGGGAC
  AAGGCACTAGACAGTCCAACCCTGCAAGGTCTTTCCTTTATTGCCAGGCCTGGTGAGTTGTTAGCCGTGG
  TTGGCCCAGTTGGAGCAGGCAAGTCGTCGCTGTTGAGCGCAGTGCTGGGTGAGCTGCCTCCTGCCAGCGG
  GCTGGTCAGCGTGCACGGGAGGATCGCCTACGTTTCTCAGCAGCCCTGGGTGTTCTCGGGCACCGTGAGG
  AGCAATATTTTATTTGGGAAGAAATATGAGAAGGAGCGATATGAGAAAGTGATCAAGGCCTGTGCTCTGA
  AGAAGGACCTGCAGCTTCTGGAGGACGGGGATCTGACGGTTATAGGAGACCGGGGAGCCACGCTGAGTGG
  AGGCCAGAAAGCTCGGGTGAACCTGGCACGGGCCGTCTACCAGGACGCCGACATCTACCTCCTTGATGAT
  CCGCTCAGCGCTGTCGATGCAGAAGTGGGCAAGCACCTGTTCCAACTGTGTATCTGTCAGGCGTTGCACG
  AGAAGATCACCATTTTAGTGACTCACCAGTTACAGTACCTCAAAGCTGCAAGCCACATCCTCATACTCAA
  AGATGGTGAGATGGTGCAGAAGGGGACTTACACGGAGTTTCTGAAATCTGGTGTAGATTTTGGCTCCCTG
  TTAAAGAAAGAAAACGAGGAAGCAGAGCCCTCCACAGCCCCAGGAACCCCGACACTCAGGAAACGAACCT
  TCTCCGAGGCCTCAATTTGGTCTCAGCAGTCATCCAGACCCTCGTTGAAAGACGGGGCCCCAGAGGGCCA
  AGACGCAGAGAACACGCAGGCAGTGCAACCCGAGGAGAGCCGTTCGGAAGGGAGAATCGGCTTCAAGGCC
  TACAAGAATTACTTCTCGGCGGGCGCATCCTGGTTCTTCATCATTTTCCTTGTGCTGCTTAACATGGTGG
  GCCAGGTTTTCTATGTTCTTCAGGACTGGTGGCTTTCCCACTGGGCGAACAAGCAAGGTGCACTGAACAA
  CACCAGAAATGCGAATGGAAATATAACGGAGACCCTAGACCTCAGCTGGTACTTAGGAATTTACGCAGGT
  CTAACTGCGGTCACCGTCCTTTTTGGCATAGCGAGATCCCTACTGGTGTTCTATATCCTTGTGAACGCTT
  CCCAGACTTTGCACAACAGGATGTTTGAGTCCATACTGAAGGCTCCCGTGTTGTTCTTCGACAGAAATCC
  AATCGGGAGGATTTTAAATCGTTTCTCCAAAGACATCGGACACATGGATGATTTGCTTCCCCTGACGTTC
  CTGGACTTCATCCAGACGTTGCTCCTCGTCGTAAGTGTGATCGCTGTGGCCGCGGCCGTGATCCCTTGGA
  TCCTCATACCATTGGTTCCGCTCTCAGTCGTCTTCCTGGTTCTTCGGAGATACTTCTTAGAGACGTCACG
  GGATGTCAAGCGCCTGGAATCCACAACACGGAGCCCGGTATTCTCCCATTTATCGTCCTCCCTCCAGGGA
  CTCTGGACCATCCGGGCTTACAAAGCTGAGGAGAGGTGTCAGGAGCTGTTTGATGCACACCAGGACTTGC
  ATTCAGAGGCTTGGTTCTTGTTCCTGACGACATCGAGATGGTTCGCTGTGCGTCTGGACGCCATCTGCGC
  CATCTTTGTAATCGTCGTTGCCTTCGGGTCCCTTGTTCTGGCGAAGACTTTGAATGCTGGGCAGGTTGGC
  CTGGCCTTGTCCTACGCCCTCACACTCATGGGGATGTTCCAGTGGTCTGTGCGACAGAGCGCCGAAGTAG
  AGAATATGATGATTTCAGTGGAGAGAGTGATTGAGTACACGGACCTAGAGAAGGAGGCGCCTTGGGAGTG
  CAAGAAGCGCCCACCCCCAGGCTGGCCCCACGAGGGAGTCATCGTCTTCGACAATGTGAACTTCACCTAC
  AGCTTAGATGGGCCTCTGGTTCTGAAGCACCTGACTGCGCTCATCAAGTCCAGGGAAAAGGTTGGAATTG
  TGGGCAGAACCGGAGCTGGGAAAAGCTCCCTCATCTCGGCCCTCTTCAGGCTGTCAGAACCCGAGGGGAA
  AATCTGGATCGATAAGATCTTGACAACCGAAATTGGGCTTCACGACTTAAGGAAGAAAATGTCAATCATA
  CCACAGGAACCTGTTCTGTTCACTGGAACCATGAGGAAAAACCTGGACCCCTTCAATGAGCACACGGACG
  AGGAGCTGTGGAGGGCCTTGGAGGAGGTACAACTTAAAGAGGCCATTGAAGATCTTCCTGGAAAAATGGA
  TACTGAATTAGCAGAATCTGGATCCAATTTCAGTGTTGGACAGAGACAGTTAGTGTGCCTTGCAAGGGCC
  ATTCTAAAGAATAACCGAATACTGATCATTGATGAAGCAACTGCAAATGTGGACCCAAGAACGGATGAGT
  TAATACAACAGAAGATCCGGGAGAAGTTTGCCCAGTGCACAGTGCTCACCATTGCTCACAGACTGAACAC
  CATCATTGACAGTGACAAGATAATGGTTTTGGATTCAGGAAGACTGAAAGAATATGATGAGCCGTATGTC
  TTGCTGCAGAATCCAGAGAGCCTCTTTTACAAGATGGTTCAGCAACTGGGCAAGGGCGAAGCCGCTGCCC
  TCACCGAAACAGCAAAACAGGTATACTTCAGACGGAATTACCCAGATATTACATTCACCAGCCCCGCGGT
  TATGAACACCTCCAATGGACAGCCCTCGGCCTTAACAATATTTGAAACAGCATTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215338 representing NM_001033336
  Red=Cloning site Green=Tags(s)
  
  MLPVHTEVKPNPLQDANLCSRVFFWWLNPLFKTGHKRRLEEDDMFSVLPEDRSKHLGEELQRYWDKELLR
  AKKDSRKPSLTKAIIKCYWKSYLILGIFTLIEEGTRVVQPLFLGKIIEYFEKYDPDDSVALHTAYGYAAV
  LSMCTLILAILHHLYFYHVQCAGMRLRVAMCHMIYRKALRLSNSAMGKTTTGQIVNLLSNDVNKFDQVTI
  FLHFLWAGPLQAIAVTVLLWVEIGISCLAGLAVLVILLPLQSCIGKLFSSLRSKTAAFTDARIRTMNEVI
  TGMRIIKMYAWEKSFADLIANLRKKEISKILGSSYLRGMNMASFFIANKVILFVTFTSYVLLGNEITASH
  VFVAMTLYGAVRLTVTLFFPSAIERGSEAIVSIRRIKNFLLLDELPQRKAHVPSDGKAIVHVQDFTAFWD
  KALDSPTLQGLSFIARPGELLAVVGPVGAGKSSLLSAVLGELPPASGLVSVHGRIAYVSQQPWVFSGTVR
  SNILFGKKYEKERYEKVIKACALKKDLQLLEDGDLTVIGDRGATLSGGQKARVNLARAVYQDADIYLLDD
  PLSAVDAEVGKHLFQLCICQALHEKITILVTHQLQYLKAASHILILKDGEMVQKGTYTEFLKSGVDFGSL
  LKKENEEAEPSTAPGTPTLRKRTFSEASIWSQQSSRPSLKDGAPEGQDAENTQAVQPEESRSEGRIGFKA
  YKNYFSAGASWFFIIFLVLLNMVGQVFYVLQDWWLSHWANKQGALNNTRNANGNITETLDLSWYLGIYAG
  LTAVTVLFGIARSLLVFYILVNASQTLHNRMFESILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTF
  LDFIQTLLLVVSVIAVAAAVIPWILIPLVPLSVVFLVLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQG
  LWTIRAYKAEERCQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAIFVIVVAFGSLVLAKTLNAGQVG
  LALSYALTLMGMFQWSVRQSAEVENMMISVERVIEYTDLEKEAPWECKKRPPPGWPHEGVIVFDNVNFTY
  SLDGPLVLKHLTALIKSREKVGIVGRTGAGKSSLISALFRLSEPEGKIWIDKILTTEIGLHDLRKKMSII
  PQEPVLFTGTMRKNLDPFNEHTDEELWRALEEVQLKEAIEDLPGKMDTELAESGSNFSVGQRQLVCLARA
  ILKNNRILIIDEATANVDPRTDELIQQKIREKFAQCTVLTIAHRLNTIIDSDKIMVLDSGRLKEYDEPYV
  LLQNPESLFYKMVQQLGKGEAAALTETAKQVYFRRNYPDITFTSPAVMNTSNGQPSALTIFETAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033336
• ORF Size: 3975 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033336.3, NP_001028508.2
• RefSeq Size: 5729 bp
• RefSeq ORF: 3978 bp
• Locus ID: 239273
• Cytogenetics: 14 E4