Tpo (NM_009417) Mouse Tagged ORF Clone

CAT#: MG215425

Tpo (GFP-tagged) - Mouse thyroid peroxidase (Tpo), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tpo
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215425 representing NM_009417
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAACACTTGGAGCTATGGCAATAATGCTGGTGGTTATGGGAACTGTAATTTTCCTCTCTTTTATCC
  TGAGAAGCAGAGACATCTTGTGTGGGAAGACCATGAAGTCCCATGTTATCAGTGCTGTGGAAACGAGCCA
  GCTCATGGTGGACCATGCAGTCTACAACACCATGAAAAGAAACCTCAAGAAAAGGGAAGTCCTTTCTCCA
  GCCCAGCTTCTCTCTTTCTTTAAGCTGCCCGAGTCCACCAGTGGGGCTATTTCCCGAGCAGCAGAGATTA
  TGGAAACATCAATACAAGTCATGAAACGTGAACAGTCACAGTTCTCCACGGATGCCTTATCAGCAGACAT
  TCTGGGCACAATTGCCAACCTGTCAGGATGCTTGCCTTTCATGCTGCCACCAAGATGTCCTGACACCTGC
  CTGGCAAATAAGTACCGGCCCATCACAGGGGCGTGCAACAATAGAGATCACCCCAGATGGGGAGCCTCCA
  ACACAGCCCTAGCAAGATGGCTGCCTCCTGTCTATGAAGATGGCTTCAGTCAGCCCAAAGGCTGGAACCC
  TAATTTCTTATACCACGGCTTCCCACTGCCCCCGGTACGGGAAGTGACAAGGCACCTCATTCAAGTTTCC
  AATGAGGCTGTGACCGAAGATGACCAGTACTCTGATTTTCTGCCGGTGTGGGGACAGTACATCGATCATG
  ACATTGCTCTCACACCACAGAGCACTAGCACAGCAGCCTTCTGGGGAGGTGTCGACTGCCAGCTGACCTG
  TGAGAACCAAAATCCTTGCTTCCCCATACAGCTTCCCTCAAACTCCTCAGGGACCACTGCATGCCTGCCT
  TTCTACCGCTCCTCCGCCGCTTGTGGCACTGGGGACCAAGGTGCTCTCTTTGGCAACCTGTCTGCAGCCA
  ATCCGAGGCAGCAGATGAATGGCTTGACCTCCTTCCTTGATGCTTCCACTGTGTACGGCAGCTCCCCTGG
  CGTTGAGAAGCAGCTGCGCAACTGGAGCAGCTCGGCAGGACTGCTGCGTGTCAACACTCTCCACCTAGAT
  GCTGGCCGTGCCTACCTGCCCTTCGCAACAGCCGCCTGCGCTCCAGAGCCTGGTACCCCACGCACCAACC
  GCACGCCCTGCTTCCTGGCTGGAGACGGTCGCGCCAGTGAGGTCCCTGCCCTGGCAGCGGTACACACCTT
  GTGGCTGCGCGAGCACAACCGCCTGGCTTCGGCCTTCAAGGCCATTAACAAGCACTGGAGCGCCAACACT
  GCCTACCAGGAGGCGCGCAAGGTGGTAGGGGCACTGCACCAGATCATCACCATGAGGGATTATATCCCCA
  AAATCCTGGGTCCTGATGCCTTCAGGCAGTATGTGGGCCCCTATGAAGGCTACAACCCCACTGTGAACCC
  TACTGTGTCCAACATCTTCTCCACTGCTGCCTTTCGCTTTGGCCATGCCACAGTCCATCCACTGGTGAGA
  CGGCTAAACACTGACTTCCAGGAGCACACAGAGCTCCCCAGGTTGCAGCTGCGTGATGTCTTCTTCAGAC
  CCTGGAGGCTTATCCAGGAAGGTGGTTTGGATCCGATAGTGAGAGGCCTCCTGGCGAGAGCAGCCAAGCT
  GCAAGTCCAAGGGCAGCTGATGAATGAGGAGCTGACCGAGAGGCTCTTCGTGCTGTCTAACGTGGGCACC
  TTGGATCTGGCATCACTGAACTTGCAGAGGGGCCGGGATCATGGCTTACCAGACTACAATGAATGGAGAG
  AGTTCTGTGGCTTGTCACGCCTGGAGACACCAGCTGAGCTGAACAAGGCCATTGCCAACAGAAGCATGGT
  CAACAAGATAATGGACTTATACAAGCATGCTGACAACATTGACGTCTGGTTGGGAGGCTTGGCTGAAAAG
  TTCTTGCCGGGGGCCCGCACTGGTCCTCTGTTTGCATGTATCATTGGGAAGCAGATGAAGGCTCTGAGGG
  ATGGGGACAGGTTTTGGTGGGAGAACACCAATGTCTTCACAGACGCTCAGAGGCAGGAACTAGAAAAGCA
  TTCACTACCTCGGGTCATCTGTGACAACACTGGACTCACCAGAGTACCTGTGGATGCCTTCCGTATTGGA
  AAGTTCCCCCAAGACTTTGAATCCTGTGAGGACATCCCGAGCATGGACCTTGAACTATGGAGGGAGACCT
  TCCCACAAGATGACAAGTGTGTCTTCCCAGAGGAGGTGGACAATGGGAACTTTGTGCACTGTGAAGAGTC
  TGGGAAGCTGGTGCTTGTATACTCCTGTTTCCATGGATACAAGCTGCAGGGCCAGGAGCAGGTCACATGT
  ACCCAGAAGGGATGGGACTCCGAACCTCCTGTCTGTAAAGATGTTAATGAGTGTGCAGATCTGACACACC
  CACCTTGCCACCCCTCTGCACAGTGCAAGAACACCAAGGGAAGCTTCCAGTGTGTGTGCACAGACCCCTA
  TGTGCTAGGTGAGGATGAGAAGACCTGCATAGATTCTGGCAGGCTACCTCGGGCATCCTGGGTCTCCATT
  GCACTGGGTGCACTTCTCATTGGTGGTTTGGCCAGTCTCACCTGGATAGTAATTTGCAGGTGGACACATG
  CTGATAAGAAAGCCACATTGCCGATCACAGAGAGAGTGACCACCCAGTCAGGATGCAGAAAGAGTCAGGG
  GAGGGGGATTTCACCACACAAGGCCGCAGCTCAAGACACTGGACAGGAACCTGCAAGTGGATCCAGGGTC
  CTCCTGTGCGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215425 representing NM_009417
  Red=Cloning site Green=Tags(s)
  
  MRTLGAMAIMLVVMGTVIFLSFILRSRDILCGKTMKSHVISAVETSQLMVDHAVYNTMKRNLKKREVLSP
  AQLLSFFKLPESTSGAISRAAEIMETSIQVMKREQSQFSTDALSADILGTIANLSGCLPFMLPPRCPDTC
  LANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPKGWNPNFLYHGFPLPPVREVTRHLIQVS
  NEAVTEDDQYSDFLPVWGQYIDHDIALTPQSTSTAAFWGGVDCQLTCENQNPCFPIQLPSNSSGTTACLP
  FYRSSAACGTGDQGALFGNLSAANPRQQMNGLTSFLDASTVYGSSPGVEKQLRNWSSSAGLLRVNTLHLD
  AGRAYLPFATAACAPEPGTPRTNRTPCFLAGDGRASEVPALAAVHTLWLREHNRLASAFKAINKHWSANT
  AYQEARKVVGALHQIITMRDYIPKILGPDAFRQYVGPYEGYNPTVNPTVSNIFSTAAFRFGHATVHPLVR
  RLNTDFQEHTELPRLQLRDVFFRPWRLIQEGGLDPIVRGLLARAAKLQVQGQLMNEELTERLFVLSNVGT
  LDLASLNLQRGRDHGLPDYNEWREFCGLSRLETPAELNKAIANRSMVNKIMDLYKHADNIDVWLGGLAEK
  FLPGARTGPLFACIIGKQMKALRDGDRFWWENTNVFTDAQRQELEKHSLPRVICDNTGLTRVPVDAFRIG
  KFPQDFESCEDIPSMDLELWRETFPQDDKCVFPEEVDNGNFVHCEESGKLVLVYSCFHGYKLQGQEQVTC
  TQKGWDSEPPVCKDVNECADLTHPPCHPSAQCKNTKGSFQCVCTDPYVLGEDEKTCIDSGRLPRASWVSI
  ALGALLIGGLASLTWIVICRWTHADKKATLPITERVTTQSGCRKSQGRGISPHKAAAQDTGQEPASGSRV
  LLCE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009417
• ORF Size: 2742 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009417.2, NP_033443.1
• RefSeq Size: 3291
• RefSeq ORF: 2745
• Locus ID: 22018
• Gene Summary: This gene encodes a membrane-bound glycoprotein. The encoded enzyme plays a central role in thyroid gland function. The enzyme functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mice with homozygous missense mutations in this gene exhibit hypothyroid dwarfism and hearing impairment. [provided by RefSeq, Sep 2015]