Parp4 (NM_001145978) Mouse Tagged ORF Clone

CAT#: MG215472

Parp4 (GFP-tagged) - Mouse poly (ADP-ribose) polymerase family member 4 (Parp4), (10ug). Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Parp4
• Synonyms: Adprtl1; ARTD4; C030027K23Rik; E230037B21Rik; Gm743; p193; PARP-4; PARPL; PH5P; VAULT3; VPARP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215472 representing NM_001145978, codon optimized.
  Due to the complexity of NM_001145978, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATTGGGCATTTTCGCTAACTGCATCTTCTGTCTCAAGGTCAAGTACCTTCCCCGACAGCAGAAAA
  AAAAGCTCCAAACAGATATTAAGGAAAACGGGGGGAAGTTTTCCTTTCTTCTCAATCCTCAGTGTACCCA
  CGTCATTGTAGACTCTGCAGACGTCCTCAGTCGGTGCCACCTGAATAGCATCCAGAAGAATGACGTTCAG
  ATCGCTAACCCTGCATTCATCCAGGATAGCGTACGGCAGAGGAGACTGCTGGACGTTAGGAATTACGATC
  CCCTGTCCCCAGCACCTGCTGCTCCCCCGGCTGAACGGAGCCGCTCTGAAGTGCAGTCTGAGTACCTTCC
  CAGCGACAACACCCCAGAAAAGGAGAACACTGAAGTTACTGAGGTGTCTGCTGAGAACGTGGAGATCCCA
  CCTTTCCTCCAGGACTTCGAGGTTGTGAAATATAATATTCTCGAAAAGGTCGGGGGTCCCGAAACCGTGG
  TGGTGGAGCTTCAGAGTTCCCAGGACCCTGAAAGCTGCCCGTTTGTCATCACCGCCCATTTCCTGCTCGC
  CGACCAGAAAACTCGACGGGAGTCAACGGGAAAGCAGACCTCTGAAGGAGCCATCGAATACTACGAGTCA
  TATGTCGAGGATCTGAAGCGACAGGGGTTCCTGCTGCAGGAGCATTTCACAGCAGAAGCAACTCAGTTGG
  CCAGCGAAAAACTGCAGGCACTGCTGCTGGAGGAGGTGATCTCCAGCGGGGCATTGAGCCAAGAGGTTTC
  AGATCTTCTCGAGGTTATCTGGACCGAGGCCCTCGGACATTTGGAGAATACACTCCTTAAGCCTGTTAAT
  TCTATGAGTTTGAATGATGTGTCTAAGGCTGAGGGAATACTGCTCCTGGTTAAAACAGCACTCAAAAACG
  GCGATAGCCCTGGGCAGCTGCAGAAGACTATGGCTGAGTTCTATAGGCTCCTCCCGCACAGGCACCCAGC
  ATCTGAGGAGGTCAATCTCAGGCTGTTGGCTCAAAAAGAGGACCTCTGCCAGTTGGTAAGAGACATGGTC
  AATGTATGTGAAACCAACCTGAGTAAACCAAATCCCCCTAGCCTCGCCAAGTACCGCGCTTTGAGGTGTA
  AGATCGAGCATGTTGACCAGAATACCGAAGAGTTCAGCCGGGTCAGGAAGGAAGTGTTGCAGAATAACCG
  GAGCGAGCAGCCCGTGGACATCCTGCAAATATTCCGGGTGGGCAGGGTAAATGAGGCCACAGAGTTCCTG
  AGTAAGCTGGGAAATGTTCGCCTGCTGTTTCACGGATCCCCGGTAAGAAACATTCTCGGAATCCTCTCCA
  GAGGGCTGCTGCTGCCAAAGGTAGCCGAGGACAGGGGTGTTCAGAGAACCGACGTGGGCAACTTGGGGAG
  CGGGATCTACTTTTCTGATTCCCTGAGCACCAGCATTAAGTACGCCCATGCCGGTGAGACTGACGGCTCC
  CGGCTGCTCGTAGTGTGTGACGTTGCCCTGGGAAAGTGCGTGAATCTGTTTAAGAAAGATTTCTCCCTGA
  CAGAAGCCCCTCCAGGGTATGACTCAGTCCATGGAGTTTCCGAAACCACAAGTGTGCCCACCGACTTCCA
  AGACGATGAGTTCGTGGTATATAAGACTAATCAGGTCAAAATGAAGTACATCGTCAAATTCTGTACCCCT
  GGGGACCAGATTAAAGAATTTCACCCCCACGAGAATACAGAGGTGGAGGAGCAAAGAGCTGAACCTAGCA
  GTGTTCCAGAGGCCGGCGACTTTCAGCTGCCCGACATTAAGCCCTTTACAAATATAAAAGCGGGTTTGCA
  GGATGCCTCCGCTAACCCCGTCCCTCTCGACTCTGTACACATCAAGGGCAGGGTCATCGACTTTGTTGCA
  CAGGTGATCGTCTTTCAGACTTATACCAACCAGTCCCACGTGCCAATAGAGGCAAAATATATCTTCCCTC
  TGGATGACAAGGCAGCCGTGTGTGGGTTCGAAGCATTCATAAACGGAAAGCACATAGTTGGGGAAATCAA
  GGAAAAAGAAGAGGCCCGACAGGAGTACCGAGAGGCCGTCTCTCAGGGCCATGGTGCATACCTGATGGAC
  CAAGACACCCCTGACGTATTTACTGTTTCAGTCGGGAATCTGCCACCTCGGGCCAAAGTCCTGATTAAGA
  TCACGTACATCACGGAGCTTTCTATACAATCTCCCGTGGCCATCTTCTTCATTCCAGGAACAGTAGCACC
  CTGGCAGCAGGACAAAGCGTTGAACGAGAACTTGCAGGATACAGTTGAAACAATCCGCATTAAGGAAATC
  GGAGCTGAGCAAAGTTTCTCCCTGGCTATGAGCATTGAAATGCCGTACATGATCGAGTTCATCAGTAGTG
  ACACCCACGAACTGAGACAGAAAAGCACAGATTGTAAAGCAGTCGTCAGCACAGTGGAAGGCAGTAGTCT
  CGATTCTGGAGGGTTCTCCTTGCATATCGGCCTGCGGGACGCTTACCTGCCTCGCATGTGGGTGGAGAAA
  CACCCTGAGAAGGAATCTGAGGCCTGCATGCTCGTGTTTCAGCCAGAGCTGGCCGACGTCTTGCCAGACC
  TGCGGGGAAAGAATGAGGTCATCATCTGCCTGGACTGCAGCTCTTCCATGGAAGGAGTAACCTTTACCCA
  AGCTAAGCAGGTGGCACTGTATGCCCTCAGTCTCCTGGGGGAAGAACAGAAAGTCAATATTATGCAATTC
  GGGACCGGGTACAAGGAATTGTTCAGCTACCCCAAGTGTATCACAGACAGTAAGATGGCAACTGAGTTCA
  TTATGTCCGCCGCACCCTCAATGGGTAATACAGATTTTTGGAAGGTGCTGAGGTATCTGTCACTGCTGTA
  CCCCAGCGAAGGATTCCGCAATATTCTGCTCATCTCTGACGGCCACCTCCAGTCTGAAAGCCTCACCCTG
  CAGCTGGTGAAGAGGAACATTCAGCATACGCGAGTCTTCACTTGTGCAGTGGGGAGCACAGCAAACAGGC
  ACATCCTGCGCACCCTGTCTCAGTGCGGAGCCGGCGTGTTCGAGTATTTTAACAGCAAGAGCAAACACTC
  TTGGAAGAAGCAGATTGAGGCCCAAATGACAAGGATCAGATCTCCCTCATGTCATTCTGTCAGCGTAAAA
  TGGCAGCAGCTCTCCCGCGATGCTCCTGAACCCTTGCAGGCCCCTGCATGGGTTCCTAGCCTTTTCCATA
  ATGATCGCCTGTTGGTTTACGGGTTCATTCCTCATTGTACCCAGGCAACCCTGCAAGCCTTTATTCAGGA
  GAAAGAGTTTTGTACCATGGTTAGCACCACCGAACTGCAGAAGACCACTGGCACTATGATCCATAAATTG
  GCAGCACGGGCACTGATCAGGGATTATGAGGACGGGATCCTGCACGATGATGAGACGAACCATGAGATGA
  AGAAGAACATCATGAAATCTCTGATAATCGAACTTAGCAAGGAGAACAGTCTGATAACACAGTTTACTTC
  CTTCGTAGCAGTGGAAAAGCGAGATGTGAATGAAATTCCGTTCGCAAACGTCCCTAATATTAGTGAACTG
  GTCGCAAAGGAGGATGTGGACTTCTTGCCATACGTTTCTTGGCAGGAGAAGCAGCCAGAGGCCAGTATTT
  CCCAGACCGAAATTGATTCATCACGCCTCAAGCACAATAAACTGTCTGATGGACATGGTGTACTTCAGCC
  AGTCAGCGTGTCTAGCGAAGTGAACGAAAAACCTAGCCTTCTGCTTGCAGCTAAAAAGCGCAAGATTAAG
  ACTATTAAGAAATGCAGCCTTGATATCAGCGAAGATTTCGAGGACCGAACTGCTGTGGCCCAGTCCCCCG
  CAACTGCACAATCCTTGAATTTCCATCTTCCTCTGTCAGTGCGCCCTCAGCTCAAGGCCGTCGAGCAGCA
  GCTGCACGGCAACAGACTGGAACCCAAACAGCGCGGAGGATTTCGGAAACTCCTGATGGCCAAAAAGTGC
  AGGAACGTGCCCGACTCCCTTGTCTCCAGCGCCCCTGCTGTTACGGCCGAGTTCTCATATCTGTCCGCAT
  GCTCCTCATCTTCTGCTTTTCTTAGCCCACTGTGCGACATCCCTTCATCACTGCCCCCCCATCCCCTCGG
  TGGTACTCATCCCCCCCCTCCACTCCCACTGCCTGACGGGACTCACCTGCCATCTCCACTCTTCGGAAGC
  ACTCATCCTCCACCACCACTGTTCGGTGGCACTCTCATCCCACCTCCTTCTAGCCTCTTCGGCGGAACAC
  ACCTTCCACCACCTCCACCCCTGCCTGGGGGAACGCACATACCACCACCTCCACCCATTCCTGGAGGAAC
  TCTCATCCCTCCCTCTAGTTCCCTTTTCGGCGGGACACATCTGCCTCCTCCGCCTCTCCTGTCCGCAGGC
  ACACATATTCCACCACCTCCTTTGCTGTCAGCTGGCACGCACTTGCCTCCTCCCCCCCTCCTGCCAGCTG
  GCACCCACATACCTCCACCACCACCCATCACCGGGTCTACTCACCCACCTCCACCGTCATCTCTGTTCGG
  GGGCACACATCTGCCGCCCCCACCCCCCCTGCCTGGCGGTACTCACATTCCTCCGCCACCTCCTATACCT
  GGCGGAACATTGATCCCATCCCCCTCCTCACTGTTCGGCGGAACACACCTTCCCCCTCCCCCTCTTCTCC
  CTGCGGGAACCCATATCCCGCCGCCCCCTCCCATCACAGGATCTACCCACCCACCCCCCCCCTCCAGCCT
  GTTCGGGGGGACCCATCTTCCTCCGCCTCCTCCCGCAGGCACTCAGTTCTCTCTGAGCCCCATAGGCTTT
  ATTCCTCCTAAGCTGGGCCCCCCAAAGCTCAGCCATAGTCACAAGTTGGTCGGCGATACCAATATACATG
  ACAGCGAGCCACCCCTGTTGGGGTTTAAAGATCTCTGTAGCCGAGACATGGGCTTCTCTTGTGGAACAGC
  ATTTTCTGGGAGCTTCGCTTCTTCCAAAGACTTCGACCCAGGGAAGTTCAGCCAGGGACCAAACAACATC
  AGCTTTTCCCCTAAGGCCCCAGAAATGGGAGTGCTTCATCAGTCTCCGTTCTGCAGCCCGCCAAAGCCAC
  CTTCCGCACCTCCATTGGTGACAAATGTGCTGTGCTCAGAGGCACCTCAATCTTATTTCCTGAATCTTCA
  GAGCGCCGCTGTTCACCAGTCCCCCAACAACCGCGTGTCAGAGATCATAATGGAATCAGTGGAGAGTTCT
  TTGCCGTCAGACTATTCTAGCCGGGATGCTTCTAGCTACCTTGCTCTCGAGGGGGCCGAGGACTCTCTCC
  TGGGTGGGTCCTCCTTTGAAACAGACACCGACGAGGCCGCCGCTTTCATAGCGAATGATCTCCTCACAAG
  CATTGAAACCAGCTCTGATGAGGAGTGTGCTTTCTGCGATGAGGACCAGGAGTCCCCCGTACCATGGGCA
  AGCCTTTTCGCACTGCAGACGGAGAACGGGTTCTGGAAGCTTACACCTGAACTTGGTCTGATCCTGAATC
  TGAACGTGAACGCTCTGCTGACCAGCCTGGAAGAGAAGGGAATTAGGAGCCTTGGCACCAAGGGCCGCGA
  ACGGCTCCTCGACTTGATAGCTACTCTGCTGGTGCTGCAGTTCCTTTACACCAAGCTGGAGCAAGAGGGA
  ATGGTGGCAAAAAGTTTGATCAAAATGGATGATGCCTTTATCAGCAGGAACATACCCTGGGCATTCGAAA
  ATATCAAGAAGGCACGGGAGTGGGCTCGCAAGACAGAGGGCCAGTACCCCTCAATTTGTCAGCGCTTGGA
  GCTCGGAAAAGACTGGGAATCTGCGACCAAGCAGCTCTTGGGAATCCAGCCTCAGGCAAATACCTCTCTG
  CACCGCATCCTGTACTACAGCCAGGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215472 representing NM_001145978
  Red=Cloning site Green=Tags(s)
  
  MTLGIFANCIFCLKVKYLPRQQKKKLQTDIKENGGKFSFLLNPQCTHVIVDSADVLSRCHLNSIQKNDVQ
  IANPAFIQDSVRQRRLLDVRNYDPLSPAPAAPPAERSRSEVQSEYLPSDNTPEKENTEVTEVSAENVEIP
  PFLQDFEVVKYNILEKVGGPETVVVELQSSQDPESCPFVITAHFLLADQKTRRESTGKQTSEGAIEYYES
  YVEDLKRQGFLLQEHFTAEATQLASEKLQALLLEEVISSGALSQEVSDLLEVIWTEALGHLENTLLKPVN
  SMSLNDVSKAEGILLLVKTALKNGDSPGQLQKTMAEFYRLLPHRHPASEEVNLRLLAQKEDLCQLVRDMV
  NVCETNLSKPNPPSLAKYRALRCKIEHVDQNTEEFSRVRKEVLQNNRSEQPVDILQIFRVGRVNEATEFL
  SKLGNVRLLFHGSPVRNILGILSRGLLLPKVAEDRGVQRTDVGNLGSGIYFSDSLSTSIKYAHAGETDGS
  RLLVVCDVALGKCVNLFKKDFSLTEAPPGYDSVHGVSETTSVPTDFQDDEFVVYKTNQVKMKYIVKFCTP
  GDQIKEFHPHENTEVEEQRAEPSSVPEAGDFQLPDIKPFTNIKAGLQDASANPVPLDSVHIKGRVIDFVA
  QVIVFQTYTNQSHVPIEAKYIFPLDDKAAVCGFEAFINGKHIVGEIKEKEEARQEYREAVSQGHGAYLMD
  QDTPDVFTVSVGNLPPRAKVLIKITYITELSIQSPVAIFFIPGTVAPWQQDKALNENLQDTVETIRIKEI
  GAEQSFSLAMSIEMPYMIEFISSDTHELRQKSTDCKAVVSTVEGSSLDSGGFSLHIGLRDAYLPRMWVEK
  HPEKESEACMLVFQPELADVLPDLRGKNEVIICLDCSSSMEGVTFTQAKQVALYALSLLGEEQKVNIMQF
  GTGYKELFSYPKCITDSKMATEFIMSAAPSMGNTDFWKVLRYLSLLYPSEGFRNILLISDGHLQSESLTL
  QLVKRNIQHTRVFTCAVGSTANRHILRTLSQCGAGVFEYFNSKSKHSWKKQIEAQMTRIRSPSCHSVSVK
  WQQLSRDAPEPLQAPAWVPSLFHNDRLLVYGFIPHCTQATLQAFIQEKEFCTMVSTTELQKTTGTMIHKL
  AARALIRDYEDGILHDDETNHEMKKNIMKSLIIELSKENSLITQFTSFVAVEKRDVNEIPFANVPNISEL
  VAKEDVDFLPYVSWQEKQPEASISQTEIDSSRLKHNKLSDGHGVLQPVSVSSEVNEKPSLLLAAKKRKIK
  TIKKCSLDISEDFEDRTAVAQSPATAQSLNFHLPLSVRPQLKAVEQQLHGNRLEPKQRGGFRKLLMAKKC
  RNVPDSLVSSAPAVTAEFSYLSACSSSSAFLSPLCDIPSSLPPHPLGGTHPPPPLPLPDGTHLPSPLFGS
  THPPPPLFGGTLIPPPSSLFGGTHLPPPPPLPGGTHIPPPPPIPGGTLIPPSSSLFGGTHLPPPPLLSAG
  THIPPPPLLSAGTHLPPPPLLPAGTHIPPPPPITGSTHPPPPSSLFGGTHLPPPPPLPGGTHIPPPPPIP
  GGTLIPSPSSLFGGTHLPPPPLLPAGTHIPPPPPITGSTHPPPPSSLFGGTHLPPPPPAGTQFSLSPIGF
  IPPKLGPPKLSHSHKLVGDTNIHDSEPPLLGFKDLCSRDMGFSCGTAFSGSFASSKDFDPGKFSQGPNNI
  SFSPKAPEMGVLHQSPFCSPPKPPSAPPLVTNVLCSEAPQSYFLNLQSAAVHQSPNNRVSEIIMESVESS
  LPSDYSSRDASSYLALEGAEDSLLGGSSFETDTDEAAAFIANDLLTSIETSSDEECAFCDEDQESPVPWA
  SLFALQTENGFWKLTPELGLILNLNVNALLTSLEEKGIRSLGTKGRERLLDLIATLLVLQFLYTKLEQEG
  MVAKSLIKMDDAFISRNIPWAFENIKKAREWARKTEGQYPSICQRLELGKDWESATKQLLGIQPQANTSL
  HRILYYSQG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145978
• ORF Size: 5907 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145978.2, NP_001139450.2
• RefSeq Size: 6391
• RefSeq ORF: 5910
• Locus ID: 328417