Cast (NM_009817) Mouse Tagged ORF Clone

CAT#: MG215608

Cast (GFP-tagged) - Mouse calpastatin (Cast), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cast
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215608 representing NM_009817
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTCGCAAGTTGGTGGTACAAGACGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAG
  GAGAAAAGAAAGGATCCGATGAGAAAAAAGCAGCAAGTTTGGGGAGCAGTCAGCCTTCCAGACCACATGT
  TGGTGAAGCTGCCACAGCCACCAAGGTGACAGCTTCCTCTGCTGCCACCAGCAAGTCCCCCAGCATGAGT
  ACCACAGAGACTAAGGCTGTGAAAACAGAGTCTAAGAAACCACAATCAAGTGAGCAACCTGTGGTTCACG
  AGAAAAAGTCAAAAGGAGGGCCAAAGGAAGGGTCAGAGCCCAAAAATCTTCCGAAGCACACATCAAGTAC
  AGGAAGCAAGCATGCTCATAAAGAAAAAGCACTTTCCAGATCAAATGAACAGATGGTGTCAGAGAAACCA
  TCAGAATCAAAGACAAAATTTCAAGATGTTCCTTCAGCTGGTGGAGAGAGTGTGGCTGGAGGTGGGACTG
  TGGCCACTGCCTTGGACAAAGTGGTTGGTAAGAAAAAAGAACAGAAACCATTCACACCAGCTTCACCTGT
  GCAGTCCACACCGAGCAAGCCATCTGACAAGTCAGGCATGGATGCTGCCTTGGATGACCTGATAGACACC
  TTAGGTGGACATGAAGATACTAACAGAGATGATCCACCATATACTGGACCAGTAGTTTTGGACCCAATGT
  ATTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTTTGGA
  AAAAAATGAAGGGATCACACAACCTCTTCCAGACTCTCCTAAACCTATGGGGACTGACCAGGCTATAGAT
  GCCTTGTCATCTGATTTCACCTGTAGTTCTCCAACTGGAAAGCAAAGTGAGAAAGAGAAGTCTACTGGGG
  AAATTTTCAAAGCTCAGTCTGCAGGAGTGACCAGAAGTTCTGTTCCCCCCAAGGAGAAGAAAAGGAAGGT
  GGAAGAGGAGGTCATAAATGATCAAGCACTTCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGAT
  CCTCCGAGCCACGTTAGCCAAGCTGAACAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAGT
  GTGGTGAAGATGAGGACACAGTCCCAGCTGAGTACAGGTTAAAACCAGCAAAGGATAAAGATGGAAAACC
  ACTATTGCCAGAGCCTGAAGAAACATCTAAGAGCCTGAGTGAGTCGGAGCTGATTGGGGAGCTTTCAGCA
  GATTTTGACCGATCTACATATCAAGACAAACCATCTACGCCAGCTGAAAAGAAATCCAATGACACATCCC
  AAACTCCTCCGGGGGAGACTGTGCCTCGGGCCTCCATGTGCAGTATACGGTCAGCGCCACCCAAACTAGC
  ATCCTTGAAGGGCGTGGTACCAGAAGATGCTGTTGAAACCTTGGCTGGAAGCCTGGGGACAAGGGAAGCA
  GATCCAGAACATGAAAAAACTGTGGAGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGAGCATGAAAAAC
  TTGGTGAAAAAGAAGAAACAGTGCCTCCTGATTATCGACTAGAAGAAGTCAAGGATAAGGATGGAAAACC
  ACTCCTGCCCAAAGAATCCCAGGAACAACTTGCACCCTTAAGCGATGACTTCCTTCTTGATGCCTTGTCT
  CAGGACTTCTCCAGTCCGGCAAACATATCGTCTCTTGAATTTGAAGATGCCAAACTTTCTGCTGCCATTT
  CTGAAGTAGTTTCTCAGACACCTGCTCCAAGCACCCATGCAGCAGCTCCACTGCCTGGCACTGAGCAGAA
  AGACAAAGAACTTGATGATGCCTTGGATGAACTTTCTGACAGTCTTGGACAAAGGCCGCCTGATCCAGAT
  GAGAACAAACCACTGGATGACAAAGTGAAGGAGAAAATCAAACCAGAGCATAGCGAGAAACTGGGAGAAA
  GAGACGACACCATCCCCCCTGAATACAGGCATCTCTTGGATAATGATGGGAAGGACAAACCAGAGAAGCC
  ACCGACTAAGAAAACAGAGAAACCTGATCAGGACCGGGACCCCATTGATGCCCTCTCAGAAGATTTGGAT
  AGCTGCCCCTCAACTACAGAGACCTCAAAGAATACAGCAAAGGGGAAGAGCAAGAAGACTTCAAGTTCCA
  AAGCATCCAAGGACGGAGAGAAAACAAAGGACTCTTCCAAGAAGACAGAGGAAGTGTCCAAGCCAAAGGC
  TAAAGAAGATGCAAGACACAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215608 representing NM_009817
  Red=Cloning site Green=Tags(s)
  
  MAFASWWYKTHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSRPHVGEAATATKVTASSAATSKSPSMS
  TTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKP
  SESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDT
  LGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQAID
  ALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQPD
  PPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGELSA
  DFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREA
  DPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALS
  QDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPDPD
  ENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKTEKPDQDRDPIDALSEDLD
  SCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009817
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009817.3, NP_033947.1
• RefSeq Size: 2894
• RefSeq ORF: 2265
• Locus ID: 12380
• Gene Summary: This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. [provided by RefSeq, Jul 2014]