Acsl4 (NM_001033600) Mouse Tagged ORF Clone

CAT#: MG215668

Acsl4 (GFP-tagged) - Mouse acyl-CoA synthetase long-chain family member 4 (Acsl4) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acsl4
• Synonyms: 9430020A05Rik; ACS4; AU018108; Facl4; Lacs4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215668 representing NM_001033600
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGAGAATAAAAGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACT
  TCGACTCACTAGCTGTCATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTAGC
  CAAATTTGGGAAGAAGGACAGCCTTGGAACCCGGGAGATCCTGAGTGAAGAAAATGAAATGCAGCCAAAT
  GGAAAGGTTTTTAAGAAGTTAATTCTTGGGAATTATAAATGGATAAACTATCTTGAAGTGAACTGCAGAG
  TGAATAACTTTGGAAGTGGCCTCACTGCATTGGGACTGAAACCAAAGAACACCATTGCCATTTTCTGTGA
  GACCAGGGCAGAGTGGATGATTGCAGCACAGACTTGCTTTAAGTACAACTTTCCACTTGTGACTTTATAT
  GCCACACTTGGCAGAGAAGCTGTAGTTCATGGATTAAATGAATCTGAGGCTTCCTATCTGATTACTAGTG
  TTGAGCTTCTGGAAAGCAAACTGAAGGCGGCCTTAGTAGATATCAATTGTGTTAAACATATCATTTATGT
  GGATAATAAGACTATCAATAGAGCAGAGTACCCTGAGGGGCTTGAAATTCACAGCATGCAATCAGTAGAG
  GAGCTGGGAGCCAAGCCAGAAAACTTGAGCGTTCCTCCAAGTAGACCAACCCCTTCAGACATGGCCATTG
  TCATGTACACCAGTGGTTCTACGGGCCGCCCCAAGGGAGTGATGATGCATCATAGCAATTTGATTGCTGG
  AATGACAGGCCAGTGTGAACGTATCCCTGGACTAGGACCGAAGGACACATATATTGGCTACTTACCTTTG
  GCTCATGTGCTGGAACTGACAGCAGAGATATCATGCTTTACCTATGGCTGTAGGATTGGATACTCTTCAC
  CCCTTACACTGTCTGACCAGTCCAGCAAAATCAAGAAGGGAAGCAAGGGTGATTGTACTGTACTGAAACC
  CACACTTATGGCCGCTGTTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAGGTTCAAGAG
  ATGAATTATGTTCAGAAAACTCTATTTAAAATCGGGTATGATTACAAATTAGAGCAAATCAAGAAAGGCT
  ATGACGCCCCTCTTTGTAATCTGATACTGTTTAAAAAGGTGAAGGCTCTGCTGGGAGGGAATGTCCGCAT
  GATGCTGTCTGGCGGCGCGCCACTGTCCCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCC
  ATTGGTCAGGGATATGGGCTGACAGAATCATGTGGTGCTGGAACAGTTACTGAAGTTACTGACTACACTA
  CTGGAAGAGTTGGAGCTCCTCTTATTTGCTGTGAAATTAAACTGAAAGACTGGCAGGAAGGTGGTTATAC
  AGTTCATGATAAGCCGAACCCCAGAGGTGAGATTGTGATCGGTGGCCAGAATATCTCCATGGGATATTTT
  AAAAACGAAGAGAAAACAGCAGAAGATTATTGTGTTGATGAAAATGGACAAAGGTGGTTTTGCACTGGCG
  ATATTGGAGAATTCCATCCTGATGGATGCTTACAGATTATAGATCGTAAGAAAGATCTGGTAAAGTTACA
  AGCAGGAGAATATGTATCTCTTGGGAAAGTAGAAGCTGCACTGAAGAATTGTCCACTGATCGACAACATC
  TGTGCTTTTGCCAAAAGTGACCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAAGTTGACTC
  TTTTGGCACAACAGAAGGGGGTAGAAGGATCTTGGGTTGATATTTGCAATAATCCCGCCATGGAAGCTGA
  AATACTGAAAGAAATTCGAGAAGCTGCAAATGCCATGAAATTGGAGCGATTTGAAATTCCGATCAAGGTT
  CGGTTAAGCCCAGAGCCATGGACCCCCGAGACTGGTTTGGTAACAGATGCCTTCAAGCTGAAAAGGAAGG
  AGTTGAAGAACCATTATCTCAAAGACATTGAGCGAATGTATGGGGGCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215668 representing NM_001033600
  Red=Cloning site Green=Tags(s)
  
  MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPN
  GKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLY
  ATLGREAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTINRAEYPEGLEIHSMQSVE
  ELGAKPENLSVPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL
  AHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQE
  MNYVQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCP
  IGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYF
  KNEEKTAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNI
  CAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKV
  RLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMYGGK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033600
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033600.1, NP_001028772.1
• RefSeq Size: 4987 bp
• RefSeq ORF: 2013 bp
• Locus ID: 50790
• Cytogenetics: X F2
• Gene Summary: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. [UniProtKB/Swiss-Prot Function]