Nars (NM_001142950) Mouse Tagged ORF Clone

CAT#: MG215750

Nars (GFP-tagged) - Mouse asparaginyl-tRNA synthetase (Nars) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nars
• Synonyms: 3010001M15Rik; AA960128; ASNRS; C78150
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215750 representing NM_001142950
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTAGCAGAGCTGTATGTATCTGACCGAGAAGGAAATGACGCAACCGGGGATGGAACCAAGGAGA
  AACCATTTAAAACAGGCCTAAAGGCTTTGATGACAGTTGGAAAAGAGCCATTTCCCACCATTTATGTGGA
  CTCACAAAAGGAAAATGAGAGATGGGATGTTATTTCTAAGTCACAGATGAAGAACATTAAAAAGATGTGG
  CACAGAGAACAGATGAAGAACGACTCTAGGGAGAAGAAAGAGGCAGAAGATAACTTACGAAGAGAAAAGA
  ACCTGGAGGAAGCAAAAAAAATTATTATTAAAAATGACCCGAGCCTGCCAGAGCCGGCCTGTGTAAAGAT
  TAGTGCATTAGAAGGATACAGAGGCCAGAGAGTGAAGGTGTTTGGCTGGGTCCACAGGTTGCGCAGGCAA
  GGAAAGAATTTGATGTTTTTGGTGTTGCGAGATGGTACGGGTTATCTTCAGTGTGTCTTGTCAGATGACT
  TGTGTCAGTGTTACAATGGAGTAGTCCTGTCCACCGAGAGTAGTGTGGCGGTGTACGGAACACTAAACCT
  AACTCCAAAGGGCAAACAGGCTCCAGGAGGCCATGAGCTGAGCTGTGACTTCTGGGAACTGGTGGGGCTG
  GCCCCGGCTGGAGGAGCTGATAACCTGATCAACGAGGAGTCTGATGTGGATGTCCAGCTCAACAACCGGC
  ACATGATGATCCGGGGAGAGAACATGTCCAAAATCCTGAAAGCGCGCTCCATGATCACCAGGTGTTTCCG
  GGACCACTTCTTCGACAGGGGCTACTGTGAAGTTACCACTCCAACACTGGTGCAGACGCAGGTGGAAGGT
  GGGGCCACGCTCTTCAAGCTTGACTATTTCGGGGAAGAAGCGTTTTTGACCCAGTCCTCACAGTTGTACC
  TGGAGACCTGCCTTCCAGCCCTGGGAGATGTTTTTTGTATAGCCCAGTCATACAGGGCCGAACAGTCCAG
  GACACGAAGGCACCTGGCTGAGTTCACTCACGTGGAAGCTGAGTGTCCTTTCCTGACCTTCGAGGACCTC
  CTGAACCGTTTAGAGGACCTAGTGTGTGATGTGGTGGACAGAGTCCTGAAGTCACCAGTGGCAAGCATAG
  TGTATGAGCTCAACCCGAACTTTAAACCCCCCAAGCGGCCTTTCCGCAGGATGAACTACTCGGATGCTAT
  TGAGTGGCTGAAGGAGCATGATGTCAAGAAAGAAGACGGGACTTTCTACGAGTTTGGAGATGATATTCCC
  GAAGCGCCTGAGAGACTGATGACAGACACCATTAATGAACCAATCCTGCTGTGTCGCTTTCCTGTGGAGA
  TCAAGTCTTTCTATATGCAGCGCTGTCCTGAGGATCCTCGACTTACTGAATCTGTGGACGTGTTGATGCC
  CAACGTTGGTGAGATTGTAGGTGGCTCGATGCGCTCCTGGGACAGTGAGGAGATTCTCGAAGGCTATAAG
  AGGGAAGGGATTGACCCCGCTCCTTACTACTGGTATACAGATCAGAGAAAATATGGCACCTGTCCTCATG
  GAGAGTATGGCTTGGGCTTGGAACGATTTCTTAGCTGGATTCTGAACAGGTATCACATCCGAGATGTGTG
  CCTGTACCCTCGATTTCTCCAGCGCTGCAGGCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215750 representing NM_001142950
  Red=Cloning site Green=Tags(s)
  
  MVLAELYVSDREGNDATGDGTKEKPFKTGLKALMTVGKEPFPTIYVDSQKENERWDVISKSQMKNIKKMW
  HREQMKNDSREKKEAEDNLRREKNLEEAKKIIIKNDPSLPEPACVKISALEGYRGQRVKVFGWVHRLRRQ
  GKNLMFLVLRDGTGYLQCVLSDDLCQCYNGVVLSTESSVAVYGTLNLTPKGKQAPGGHELSCDFWELVGL
  APAGGADNLINEESDVDVQLNNRHMMIRGENMSKILKARSMITRCFRDHFFDRGYCEVTTPTLVQTQVEG
  GATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAEFTHVEAECPFLTFEDL
  LNRLEDLVCDVVDRVLKSPVASIVYELNPNFKPPKRPFRRMNYSDAIEWLKEHDVKKEDGTFYEFGDDIP
  EAPERLMTDTINEPILLCRFPVEIKSFYMQRCPEDPRLTESVDVLMPNVGEIVGGSMRSWDSEEILEGYK
  REGIDPAPYYWYTDQRKYGTCPHGEYGLGLERFLSWILNRYHIRDVCLYPRFLQRCRP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142950
• ORF Size: 1677 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142950.1, NP_001136422.1
• RefSeq Size: 2663
• RefSeq ORF: 1680
• Locus ID: 70223