Pde1c (NM_001159953) Mouse Tagged ORF Clone

CAT#: MG216137

Pde1c (GFP-tagged) - Mouse phosphodiesterase 1C (Pde1c) transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde1c
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216137 representing NM_001159953
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTCCAACCAAGGAAATTGAAGAATTCGAGAGCAACTCTCTGAAACACCTGCAACCTGAGCAGA
  TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGGTTACGGTCTTT
  GGTCAAGCAATTAGAACGAGGAGAGGCCTCTGTGGTAGACCTTAAGAAGAATCTGGAATATGCAGCCACA
  GTGCTTGAATCTGTATATATTGATGAAACAAGGCGACTGCTGGATACAGAGGATGAGCTTAGTGACATTC
  AATCAGATGCTGTGCCTTCAGAAGTTCGGGATTGGCTGGCCTCCACCTTCACACGGCAGATGGGGATGAT
  GCTTAGGAGAAGTGATGAGAAGCCCAGGTTCAAGAGCATCGTCCATGCGGTGCAAGCTGGGATATTTGTG
  GAAAGAATGTACAGACGGACATCAAACATGGTTGGGCTGAGCTATCCACCGGCTGTAATCGATGCATTGA
  AGGATGTGGATACGTGGTCCTTCGATGTCTTTTCCCTCAATGAGGCCAGTGGAGATCATGCACTGAAGTT
  CATTTTCTATGAATTACTCACACGTTATGACCTGATCAGCCGTTTTAAGATACCGATTTCTGCACTTGTC
  TCATTTGTGGAGGCCCTCGAAGTGGGTTACAGCAAGCACAAAAATCCTTACCATAACCTGATGCATGCAG
  CTGACGTCACACAGACTGTGCATTACCTCCTTTATAAGACAGGAGTAGCAAACTGGCTGACAGAGCTGGA
  GATCTTTGCAATAATCTTTTCGGCTGCCATCCATGACTATGAACATACTGGAACTACAAACAATTTCCAC
  ATTCAGACTCGGTCAGATCCAGCTATCCTGTACAATGACAGATCTGTCCTGGAGAACCACCACCTGAGTG
  CAGCCTACCGCCTTCTGCAGGAAGACGAAGAGATGAATATTCTGGTTAACCTCTCAAAGGATGACTGGAG
  GGAGTTTCGAACTTTGGTTATTGAGATGGTAATGGCCACAGATATGTCCTGTCATTTCCAGCAAATCAAA
  GCCATGAAGACAGCCCTGCAGCAACCAGAAGCAATTGAGAAGCCGAAAGCCTTATCCCTCATGTTACACA
  CAGCAGACATCAGTCATCCTGCGAAAGCATGGGACCTGCACCACCGCTGGACCATGTCTCTCCTGGAGGA
  GTTCTTTAGACAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCATTTTCTCCTCTTTGTGACAGAAAGTCA
  ACCATGGTTGCTCAGTCACAAGTGGGTTTTATTGACTTCATTGTGGAGCCCACCTTCACTGTGCTCACGG
  ACATGACCGAGAAGATTGTGAGTCCATTAATTGACGAAAGCTCCCAGACTGGTGGGACAGGGCAGAGGAG
  ATCAAGTTTGAACAGCATCAACTCATCAGATGCAAAGCGATCCGGTGTCAAGAGTTCTGGGTCAGATGGA
  AGTGCTCCCATCAACAATTCTGTCATTCCTGTTGACTATAAGAGTTTTAAAGCCACTTGGACTGAGGTGG
  TGCAGATCAATCGGGAGCGGTGGCGAGCCAAGGTACCCAAAGAAGAAAAAGCCAAGAAGGAAGCTGAAGA
  GAAGGCTCGCCTGGCTGCTGAGGAAAAGCAAAAGGAAATGGAAGCCAAAAGCCAAGCTGAACAAGGCACA
  ACCAGCAAAGGCGAGAAAAAGACATCAGGAGAAGCCAAAAGTCAAGTCAATGGAACACGCAAGGGTGATA
  ACCCTCGTGGGAAGAACTCCAAAGGAGAAAAGGCAGGCGAAAAGCAGCAGAACGGTGACTTGAAAGACGG
  TAAAAATAAGGCAGACAAGAAGGATCACTCCAACACCGGAAATGAGTCAAAGAAAACAGATGGTACAAAG
  AAGCGTTCTCATGGCTCACCTGCTCCGAGCACTAGTTCCACAAGTCGCATTACCTTGCCAGTCATCAAGC
  CTCCTTTGCGTCACTTTAAGCGTCCTGCTTATGCATCTAGCTCCTATGCACCTTCCGTCCCGAAGAAAAC
  TGATGATCATCCTGTAAGATACAAGATGCTGGATCAGCGGATCAAGATGAAAAAGATCCAGAACATCTCT
  CATCACTGGAACAAAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216137 representing NM_001159953
  Red=Cloning site Green=Tags(s)
  
  MESPTKEIEEFESNSLKHLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAAT
  VLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFV
  ERMYRRTSNMVGLSYPPAVIDALKDVDTWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALV
  SFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFH
  IQTRSDPAILYNDRSVLENHHLSAAYRLLQEDEEMNILVNLSKDDWREFRTLVIEMVMATDMSCHFQQIK
  AMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKS
  TMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDESSQTGGTGQRRSSLNSINSSDAKRSGVKSSGSDG
  SAPINNSVIPVDYKSFKATWTEVVQINRERWRAKVPKEEKAKKEAEEKARLAAEEKQKEMEAKSQAEQGT
  TSKGEKKTSGEAKSQVNGTRKGDNPRGKNSKGEKAGEKQQNGDLKDGKNKADKKDHSNTGNESKKTDGTK
  KRSHGSPAPSTSSTSRITLPVIKPPLRHFKRPAYASSSYAPSVPKKTDDHPVRYKMLDQRIKMKKIQNIS
  HHWNKK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159953
• ORF Size: 2118 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001159953.1, NP_001153425.1
• RefSeq Size: 3907
• RefSeq ORF: 2121
• Locus ID: 18575
• Gene Summary: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP. [UniProtKB/Swiss-Prot Function]