Poli (NM_011972) Mouse Tagged ORF Clone

CAT#: MG216260

Poli (GFP-tagged) - Mouse polymerase (DNA directed) iota (Poli) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Poli
• Synonyms: Rad30b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216260 representing NM_011972
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGGAGTCGGAGGAGGAAGGCGGCCCCGCGGAGGAAGAAGACGCTCCTCGTGCCATGGAGCCCT
  TGCACGCGGGGGCGGCGGGCAGCTCGCGGGCAGTTTGCAGTCAAGGGCCACCTACACAAATCTCTTCGTC
  GAGAGTCATAGTCCACGTAGATCTGGATTGCTTTTATGCCCAGGTCGAAATGATCTCCAACCCAGAATTA
  AAGGACAGACCTTTAGGTGTGCAACAGAAATACTTGGTGGTTACCTGCAACTATGAAGCCAGGAAACTGG
  GTGTGAGGAAGCTTATGAATGTGAGAGATGCGAAGGAAAAGTGTCCTCAGCTGGTCCTCGTGAACGGAGA
  AGACCTGAGCCGCTACAGAGAGATGTCCTATAAGGTCACAGAATTGTTGGAGGAATTTAGTCCAGCTGTT
  GAGAGACTTGGATTTGATGAAAATTTTGTGGATCTAACAGAAATGGTGGAAAAAAGACTCCAGCAGCTTC
  CAAGTGAGGAGGTTCCGTCAGTGACTGTGTTCGGCCATGTTTACAATAACCAGTCTGTGAACCTACACAA
  CATCATGCATCGGAGACTTGTGGTTGGATCGCAGATTGCAGCAGAGATGCGGGAAGCCATGTATAATCAG
  CTCGGGCTCACAGGCTGCGCCGGAGTGGCTCCTAATAAACTCTTGGCAAAGTTAGTGTCTGGGGTTTTTA
  AACCAAATCAGCAGACGGTCTTACTACCTGAAAGTTGTCAACATCTCATTCACAGTTTGAACCACATAAA
  GGAAATTCCTGGTATCGGCTATAAAACTGCCAAGCGTCTCGAAGTTCTGGGAATCAATAGTGTTCATGAT
  CTCCAAACCTTTCCAATCAAAACATTAGAAAAAGAATTAGGAATTGCAATTGCTCAGCGTATCCAGCAGC
  TCAGTTTTGGAGAGGACAAGTCTCCCGTCACACCGTCAGGGCCACCACAGTCCTTTAGTGAAGAAGATAC
  GTTTAAAAAGTGTTCCTCAGAAGTGGAAGCTAAAGCTAAGATTGAAGAATTACTTTCCAGCCTTTTGACC
  AGAGTATGCCAGGATGGAAGGAAGCCCCATACAGTAAGATTAGTCATCCGTCGGTACTCTGACAAACACT
  GTAATCGAGAGAGTCGTCAGTGCCCTATCCCATCCCACGTCATTCAGAAGTTAGGGACAGGAAATCATGA
  CTCCATGCCTCCCCTGATTGATATCCTTATGAAACTTTTCCGAAATATGGTGAACGTGAAGATGCCCTTT
  CACCTGACTCTTATGAGCGTGTGCTTCTGCAACCTGAAAGCCCTGAGCAGTGCTAAGAAAGGGCCTATGG
  ACTGCTACCTAACGTCCCTCTCAACCCCTGCCTACACCGACAAGCGCGCCTTTAAAGTGAAGGACACCCA
  CACGGAAGACTCTCACAAGGAGAAAGAAGCAAACTGGGATTGTCTACCAAGTAGAAGAATCGAAAGCACA
  GGAACGGGGGAGTCTCCGTTGGATGCCACCTGTTTTCCTAAAGAAAAAGACACAAGTGACTTGCCCCTCC
  AGGCACTGCCAGAGGGTGTTGATCAAGAAGTCTTTAAGCAACTTCCAGCAGATATTCAAGAAGAAATCCT
  TTCTGGAAAATCTAGAGAAAATCTAAAAGGGAAAGGAAGTTTAAGTTGTCCGCTGCATGCCTCTAGAGGA
  GTATTGTCTTTCTTTTCTACAAAGCAAATGCAAGCCAGTCGCTTAAGCCCCAGAGATACTGCGCTCCCTA
  GCAAGCGGGTATCAGCTGCATCTCCCTGTGAGCCGGGAACGTCAGGACTGAGCCCCGGGAGCACCTCCCA
  TCCATCGTGCGGAAAGGACTGTTCCTATTACATAGACAGCCAGTTAAAGGATGAGCAAACGAGTCAAGGC
  CCTACTGAGTCTCAAGGATGCCAGTTTTCCAGCACGAACCCTGCTGTTTCTGGTTTCCATTCCTTTCCTA
  ATCTGCAGACTGAACAGCTCTTCTCCACACATCGCACTGTAGACAGCCACAAGCAGACAGCCACCGCCTC
  TCACCAGGGACTAGAAAGTCACCAGGGACTAGAAAGCAGAGAGCTGGATTCTGCGGAAGAAAAGCTTCCT
  TTCCCACCAGACATTGATCCGCAAGTTTTCTATGAGCTTCCAGAAGAGGTCCAAAAGGAACTGATGGCGG
  AATGGGAGAGAGCTGGAGCTGCGCGCCCCTCGGCGCACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216260 representing NM_011972
  Red=Cloning site Green=Tags(s)
  
  MGVESEEEGGPAEEEDAPRAMEPLHAGAAGSSRAVCSQGPPTQISSSRVIVHVDLDCFYAQVEMISNPEL
  KDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVTELLEEFSPAV
  ERLGFDENFVDLTEMVEKRLQQLPSEEVPSVTVFGHVYNNQSVNLHNIMHRRLVVGSQIAAEMREAMYNQ
  LGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRLEVLGINSVHD
  LQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEVEAKAKIEELLSSLLT
  RVCQDGRKPHTVRLVIRRYSDKHCNRESRQCPIPSHVIQKLGTGNHDSMPPLIDILMKLFRNMVNVKMPF
  HLTLMSVCFCNLKALSSAKKGPMDCYLTSLSTPAYTDKRAFKVKDTHTEDSHKEKEANWDCLPSRRIEST
  GTGESPLDATCFPKEKDTSDLPLQALPEGVDQEVFKQLPADIQEEILSGKSRENLKGKGSLSCPLHASRG
  VLSFFSTKQMQASRLSPRDTALPSKRVSAASPCEPGTSGLSPGSTSHPSCGKDCSYYIDSQLKDEQTSQG
  PTESQGCQFSSTNPAVSGFHSFPNLQTEQLFSTHRTVDSHKQTATASHQGLESHQGLESRELDSAEEKLP
  FPPDIDPQVFYELPEEVQKELMAEWERAGAARPSAHR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011972
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011972.2, NP_036102.2
• RefSeq Size: 2482
• RefSeq ORF: 2214
• Locus ID: 26447