C2cd3 (NM_001017985) Mouse Tagged ORF Clone

CAT#: MG216603

C2cd3 (GFP-tagged) - Mouse C2 calcium-dependent domain containing 3 (C2cd3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C2cd3
• Synonyms: AU020772
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216603 representing NM_001017985
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACAACGGAAAGGCCAAGGGCCTGGGGGCGGCCGTGGGCGCAAAAAGAGAGGTTTGAGTGACATTT
  CACCGTCTACAAGCCTGCCACCTCTGGTTGAAGGGCAGTTACGCTGCTTTCTCAAACTCACTATTAATAA
  GGTTGTATGGAAGATTGCGAAGCCTCCTACTTCTGTACTTGTCCGAGTAAGGTGGTGGGGAGAAACATCC
  GATGGAACCCTCTTTTGTCCCAGAGATGCATTACAGACTGAGCCAAAAGCTGTGAGAACAACTACCCGTT
  ACGGGATTCGCTGTGGTCCAAAGCAGTTTACCTCGTATCTGACAGATATGGCTGTGCTGGTGTTGGAAGT
  AATCACCAAGTTTGATCATCTTCCTGTTGGCAGAGTTCAGATCAGTGGACTTGCCCAGCTGTCTCCAACC
  CATCAGATCAATGGGTTTTTTACCATTGTTTCACCAGCATCGAAGAAACTTGGAGAACTTCAGGTCTCGC
  TTGCCCTGGAGCCTCTGTCAGAAACTTATGACAGCTACAAACCTCTTCCTGCCACTGAAGTGACAAAAAA
  TGTGCTTTTATCTGAGCGAGAACTCAGAGAGAATACTGAATCCAGCAACACCCAGTCTATGATCCCGTCA
  AGGTCTTGTAGGGGACCTGCCATCAAAATTGATGGGAAAGAATTAGCAGGCCACAGCAGCAGGTCAACCA
  CGCCAAGGGGAAAGGACCACTTGTACTTTGCAGAGAACTCCGATGCAGTAAAGGGTTCTTTGTGTGGACT
  ACAGCAGCACCTTAATCAAGGGACGAATGTAGAGACTATAACTCTGAGGGGCAAAGCCCCACAGAAGCAG
  CTGTCCCTCCTGAACAGCTCTGAGTTTCAGCCACAAATTAGTACAGTCGCCAAGAGTCACAGTGACTCAT
  GCATCCTGTCTTCAAACACCCCCCCTGCAAAGGACCTTCTTTCAGCTCTGTTAGAACAAGGCAATAAGCT
  GCGGAATGCCATGTTGATTTCTGCAATGAACTCCAACCCAGATACTAGTATGCTGCTTGACAAAGTCCCT
  CCTCCTATGACAGAAGCTATTCCCAGATCGTCAGCACTGAACTCATCTGAGAATCACTTTAAAGGCCACA
  GTGCAGACCACCTTCTCCCCTTGGCGGACACCGGAGCTATACAGCTGCTGTTGGGCAGTGCTGAATTGTC
  CCAAGGTCATTTCTGGAATGGATTAGGCTCTCCTCCAGATTCCCCGACTCCTGGGAGTGATGAGTACTGC
  AGCAGTGACCTGAATGATCCTCAGTATGACCAGAGTCTGCTGGAGAACTTGTTTTACACAGTGCCTAAAT
  CTGATGTCGGCACCAGTGAGCTCCCCAGTGAAGATGATGGAGTGGAACCTTCTAGAACAATGAACCAGTC
  CAAAGCCTCTGGCAGATCTAAGGTTGTAGAGTCAAAAGAGCAGAAGCAGAAGAGGGCAGCTGTCAAGAAA
  AGCAGAAACCCAATTGACCAGCAAGAGCTCTCAAGAACTCCAGGACATACCCCAGCGATGTCACTAAGTG
  TGGATAGACTGGCTCTTCTGGGTAGAGTACATTCGGTCAGAATCATCGTTGAAACCATGGGAGTCCCTCC
  AGATAGTCCCCATATGACACCAAGCAGGAAGAACTTTGCCGGGAAACCACCAAAGCCAACAGCAGCAAAA
  AAGCGCACTTTCTTTGTGGAGTATCACTTTCCTGTGGGCTTTTCCAAAAGTGGACTGGGAAAGACAGCTT
  TGATCACTGAGGTTGTTAGACTTGCCTCCAGTAAAATCACCGATGGAGTGGTGAAATTCCAGCAGCGATT
  TGTGTGTCCAGTAGAGTTTGGTGGCCCGATGATAGAGCACTGGTGGGATTCTAACCTCATCTTCCAAATT
  TATGCAAAGAAAACCCCACAGAAAAAGCCAGAAGTCATTGGATCTGCATCTCTCCCTTTGCGAGCTGTCA
  TTCAGTCAGAGCTGCTTTCTTTCAGTAGCCAGCTTCCAGTGCAACAGGAAAATGGTCTGTCTTCACTTGG
  CCCCCTAAAGGTCACCATGGAGCTTGTTTTAGGCCACAAGGATTTCACTGGAATCAGTGCTAAGTTAAGT
  AGCAGCACCCAACCTGCTCCAGTTTCTGCTGCTACAAGTTCAGATACGATACTACCAGAGACAGGCCAGG
  ATACCGCCTGTACCAGAAATCCACAGAGCTCAAATAAAATTCACGAGGAAACCACAAAGAAAACACAGAA
  TTTAGTGCTCCCCGACCAAGAGTCAGCAAACTCTGTAGCCTCAAACTCTTCAATCTTCATGGCTGTGCCA
  TCTTGCAATTTAGTCCATCAAATAAATGGCTCAAACAAAGAAAGCGGTTTGCTGTTACATGTGCTGTTGA
  TGGTGCCAGATGGGAAAGATTTTGTTTTTGGAGAACGGGAGAAACAACCATCATGCAATGTTTATTTGAA
  TTGCAAGCTTTTCAGCACAGAAGAAGTCACCAGATCTGTCGTCTCGTGGGGCACAGCACAGCCTGTCTTT
  AATTTTTCTCAGGTGATCCCTGTCTCTCTGACTTCCAAATGCCTGGAGAGACTTAAGAACAATGTGATGA
  TAATTGAAACTTGGAACAAGGTGCGGAGCCCAGGACAGGACAAGCTGCTCGGGCTGGTGAAACTGCCACT
  CCATCAGTTTTACATGTCATTCAAAGACCCCAAGATTTCTCGCCTGCTGCTTGATGCCAGGTACCCAGTT
  GTTGCTGTTGACAGCTATATGCCTGTGATTGATGTGTTCTCGGGCCACCAAAATGGGAGTCTTCGAGTCT
  TTTTAGCCATGGGTTCTTCAGCTCAGATAATGATGCTGCAAAGATTAAAGAATGAAGAAGGAACACTCCC
  TCCCTTCAGTCCTAGGCCAGCCCATTTCCTGGACCAGCCACCTGTAGCATCTGTTGCTATGCCAGAAAAA
  CAAGGAACCCGGTTGATGGAGCACCACTTTGAGTTCTGTGTGGCGATGGTTAAAGGGCTCATGCCCCTTC
  AGGCAACAGTCTGGGGAGAAGCAGATTGCTACGTCCAGTACTACTTTCCATTTCAAGACTCCCAACCCAG
  TGTGCTCCAAGGACCCGACTTCCTTGAAAATGGAATTACTCTGAAGCCCTTTAGAACTTCAACCACACTG
  TGTGTCCCAGATCCCGTCTTTAATAGTGAACACCATCACTCTCTCCTGTTGCCGACTGACGTTCCAGTGC
  AAAGGCTCCTGCTGAGTGCCTTCTCCTCCCAGGGGCTGGTGCCTGGAGGAGGCGTCCAGTTTGAAGTCTG
  GTGCAGATACTATTATCCTAACGTAAGGGACCAGATGGTTGCCAAAGGAACCTTGCCATTATCAAGGGTC
  TGTGCCATGGTAACCATGCAGTACCGTGAAGATGTGGGAATGCAGAGCTTTAATCTCCCATTAACCTCCA
  GACTTGAGCACAGCAAAGAATTAAAGAACCAGTCATCAGGTTTCCTGGATGTGGGTTTGAGGTACCGGCG
  TAGCCCAAGAACAGCAGAGGGAATTCTTGCTGCCCGAGCTGTCTCCATCTCAGTCCATATTATCAGAGCC
  TGTGGTCTTCAGGCAGCAGCCAAGGCGTTGGCTGAGCAGGAACCCGCTTTACAGTTTAGCGCCACCGTTG
  GAGTCAATGCTTCTGTCACCGCCCATCTCTCATTTCTGCCCAAGGGGGAGCAGCGCCAAACCCGCCCTGT
  GGCCTGCTCCTTCTGCCCTGAGTTCTCCCACCACATTGAGTTCCCGTGTAACCTGGTGACTCAGCACTGT
  AGTGGAGAAGCCTGCTTCCTGGCAGAGCTGTTGGAGTTTGCAGAAATTATTTTTGCTATCTATCATGAAA
  ATACCAAATCAGTCAGTGATATAACCAGTATCCAGTCGTGCAAGGATTATCTGCTTGGAATAGTAAAGGT
  TCCAACGAAAGATCTGCTGGTCAAAAGATCTGGGATCACAGGATGGTATCCTGTCATTTTACCAGAAGAC
  AAAGGCCTGCCTCAGGACCTGGACTTGATGCAGAAGATTGTAGGAGGCTTGGAGCTTTCTGTTTCCTTCG
  CACATCCGGGAGATAGAGAGAGGGTACTGGAAGCTGCTGAGCTGCTGGGCTGGAGCTTTGAGAGCATCCC
  AAAGGATCTTGTCAAGAAAGAAGAAGAGGTGCCAGCCACTGTCACCATCTCTACCCCAAGGCTCTGGCTT
  CCTATCCACTGTGTGCTGCTTGCTGGTCATATGAACATTCATAAGAATACATATTGCTACCTTCGCTACA
  AGCTGTACAATCAAGAAGCCTTTTGGACTCCTCTCAGGAAACCTAAGGAGTCTACAAACAAAAACCAGGT
  CCTGATTACTTTCAAGGCATCCAAAAGAGCAGAAGTCACTAGGAGCCAGTCCCTGCTTTGGTACTTCAGG
  GAAGAGAAGTTAGAGATCCAAGTGTGGCGAGCCTATGGCAACGACAATTTAGAGAGGCCACACCAGACTG
  ACAGCTGGATTGGCTCAGCCTATGTAGACCTGTCCAGACTTGGGGAAAAATCTCCAAGGACACTGACTAT
  CAGTGGGGTGTATCCCCTGTTTGGAAGAAATGCTTCCGACCTCTCTGGAGCTGCTCTGCGCATTCATGTG
  CTTCTTTCCCCGCTTTCCCCACACACTGAACCTGCTCGGGAGCTGGACTCCATGGACTGCAGCAGCCACA
  GTGAGTCTGAGCAGCACCCCAGAAAGAGCGATGCGCTCCAGCTCTCTCCACCCCATGTGCTCCAGACGTC
  TCCCACCTCCACCCAGGTCCACGGCAACAGTGCTGCAGCCCAAGTGTGCCCCGCCCAGGAGGGCCCTCCT
  GAGTTGGCTGGGACTTTTGCAGTCAGCATCCTGGTAGAGAGAGCCATGCATCTGAGCTTGAAAGGGAGCC
  CCTTGACAGACCGGAAAGTATCTGTACCCAGTTGCTGTGTATCCTTTGCCACAGCCACTGAGCTGTCTCC
  TGTCTACACTCACGTGGTTGAAAACACGGATTCCCCCATCTGGGGTTTTCACCAGCAGGCAAGGCTTTCA
  AAAGAGCTGCTTCTGGACCCACATCAAACCCTGGTCTTCAAAGTGTGGCATAAAGGAGATGAAGAGAGGG
  TGGTTGGCTTTGCCTCGGTGGACCTCTCCCCACTACTGTCTGGCTTTCAGTTTATCTGTGGCTGGTACAA
  TATTACAGACTTCAGTGGAGAGTGCCAAGGGCAAATCAAAGTTGCTATTTCACCTATGGAGAGCCTGATG
  CACCTAAAAGAAGAAAGACAAGCAAGACGTGGGATAGATACTCCAGGAGCACTGATTCCATTGTTTAGCG
  CCCTTTCCTTCCCTGCCTCTGCTGGATGCGATGCATTCCCTAGGCCCATTGCAAGGCACGTCGAAGGCCA
  GCTTGCTCACACATCTCCAAAGGAGGACGGTCTCTCCTCTCCTGCAAGAAATGGTGCCATCAGAAGCCAG
  GCCGCACGCCATGAAGAGCATGTGCAGAACATCCGCCGCTTTCACGAGTCCCTGCAGCACGGGGAGGCAG
  TCCTGACAAGTGATGAGAAGCTGACCACAGCGCCTTCGTCCTCCCACACCTCCATCCTGACTTCTCTCAG
  GAAGAATCTGAGTGAGCTAGATGAGATCCAGAAGTACTTCAGCCAGAAGCTCAGCAAGCCCTTCCTGCCC
  TTCTCCTCTCAGAGCTCGCCTGCTGTCTCACAGAGCCAGGAGAGCCAGAGGGACCCTGTGGCTGCAGGAA
  CCGGCCGCCAGGACCCCGAGAACCAGTGCATCCTGGAGAAGTCCAATCACCTGGTGTCGCAAGTCAGCTC
  CTTGATCTCAGATCTGCAGACTCTCACCAGAGGTTCACAAGCAGCTTTGACTTCTCAGCAAGCCAGGAGT
  AGAAGCAGAGCAGTCACCACCATCCCAGATGCTCAGGGCACCGAAGCTGCTGGAGAAGGAAGCACCACAC
  TGGAGGAGCCTTTGGCAGGAGCTATAGAGGCGAGCACAGATTCCCTGCCTCCCCCTGTTGAAGAGCCTTC
  CAAGGGAGGAGGGATGCTCCATGAGTCGCTGGAGCAGACGATGCCCATCACGAGGGTGCAGAGCATCGAT
  GACACGGAGGTGGGCCCAGACTACAGTGATGAAGACTATGAGGAAGACATTATTGAGCCCAGGACCCTAA
  ATGAAATAACCACAGTGACAGACAGAACCAGCCCCTGGTCCAGTTTCATGTCAGACATGAGTGAGGTCCT
  TAGCCCTCAGCCCACTGAGGTACAAAGGGAAGGCCCCTCATGTCCCCCAGAACCTTTCCCCAGAGAGGAG
  CTGAAGGTCAAAAGTAGCCCGCAAAAGGCAGTCAGCCCCCAGCCTGCACAGGGCTCCCCTAGCCAAAGTG
  GTGTTTGTGAGGGTGGAGCCTATAAGATCGAGGTTGAAGACCTTGCCTCAGCCAAGCCTCAGCCTGTCCC
  AAGCCTAACATTCTCAGAAGCCCAAGAGGGCAGCGATTCTGTTGGATGGCGTGCATCACAGATAAATCAG
  GTCAGGAAGCCCATGCCAGAGATGCTGGCTGAGAGTGAGGCTTTCTCCAGTGAGTTCAGTGACTCTTCTG
  AAAGCTTTGAGACGTTTCCTCTACACCTACCCTCCCAAAGCAAAAGAGAAGACTACAAGGACTCCCCTGC
  TGTGAGGCAGAAGCAGGTACCAACTGGATCAGAGGTGTCCACAAGGCAGACGCTCCTTCTCCCAGAACCT
  GTTGTGGTGCCCAACTTCTTTTTGCCTCCCCAACAACTGGAGGCCTCTCTCCGCATGATCTCACACTCTC
  CAGGTTTGCCACCAGCAGCCACAACTGACCAGGACAAGAGTGAAGCCACCAGGGGAGCCCTTGCCCAGAG
  GCCTTGTCGCCCCAGACCTTACTCCATCCCTCCCAACCTTCCTGAGGAAGAGACTCGCAGGATTGCACGG
  ATATTTTCTTCCCAGTATTCCAAAAAGACTGAGGAAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216603 representing NM_001017985
  Red=Cloning site Green=Tags(s)
  
  MKQRKGQGPGGGRGRKKRGLSDISPSTSLPPLVEGQLRCFLKLTINKVVWKIAKPPTSVLVRVRWWGETS
  DGTLFCPRDALQTEPKAVRTTTRYGIRCGPKQFTSYLTDMAVLVLEVITKFDHLPVGRVQISGLAQLSPT
  HQINGFFTIVSPASKKLGELQVSLALEPLSETYDSYKPLPATEVTKNVLLSERELRENTESSNTQSMIPS
  RSCRGPAIKIDGKELAGHSSRSTTPRGKDHLYFAENSDAVKGSLCGLQQHLNQGTNVETITLRGKAPQKQ
  LSLLNSSEFQPQISTVAKSHSDSCILSSNTPPAKDLLSALLEQGNKLRNAMLISAMNSNPDTSMLLDKVP
  PPMTEAIPRSSALNSSENHFKGHSADHLLPLADTGAIQLLLGSAELSQGHFWNGLGSPPDSPTPGSDEYC
  SSDLNDPQYDQSLLENLFYTVPKSDVGTSELPSEDDGVEPSRTMNQSKASGRSKVVESKEQKQKRAAVKK
  SRNPIDQQELSRTPGHTPAMSLSVDRLALLGRVHSVRIIVETMGVPPDSPHMTPSRKNFAGKPPKPTAAK
  KRTFFVEYHFPVGFSKSGLGKTALITEVVRLASSKITDGVVKFQQRFVCPVEFGGPMIEHWWDSNLIFQI
  YAKKTPQKKPEVIGSASLPLRAVIQSELLSFSSQLPVQQENGLSSLGPLKVTMELVLGHKDFTGISAKLS
  SSTQPAPVSAATSSDTILPETGQDTACTRNPQSSNKIHEETTKKTQNLVLPDQESANSVASNSSIFMAVP
  SCNLVHQINGSNKESGLLLHVLLMVPDGKDFVFGEREKQPSCNVYLNCKLFSTEEVTRSVVSWGTAQPVF
  NFSQVIPVSLTSKCLERLKNNVMIIETWNKVRSPGQDKLLGLVKLPLHQFYMSFKDPKISRLLLDARYPV
  VAVDSYMPVIDVFSGHQNGSLRVFLAMGSSAQIMMLQRLKNEEGTLPPFSPRPAHFLDQPPVASVAMPEK
  QGTRLMEHHFEFCVAMVKGLMPLQATVWGEADCYVQYYFPFQDSQPSVLQGPDFLENGITLKPFRTSTTL
  CVPDPVFNSEHHHSLLLPTDVPVQRLLLSAFSSQGLVPGGGVQFEVWCRYYYPNVRDQMVAKGTLPLSRV
  CAMVTMQYREDVGMQSFNLPLTSRLEHSKELKNQSSGFLDVGLRYRRSPRTAEGILAARAVSISVHIIRA
  CGLQAAAKALAEQEPALQFSATVGVNASVTAHLSFLPKGEQRQTRPVACSFCPEFSHHIEFPCNLVTQHC
  SGEACFLAELLEFAEIIFAIYHENTKSVSDITSIQSCKDYLLGIVKVPTKDLLVKRSGITGWYPVILPED
  KGLPQDLDLMQKIVGGLELSVSFAHPGDRERVLEAAELLGWSFESIPKDLVKKEEEVPATVTISTPRLWL
  PIHCVLLAGHMNIHKNTYCYLRYKLYNQEAFWTPLRKPKESTNKNQVLITFKASKRAEVTRSQSLLWYFR
  EEKLEIQVWRAYGNDNLERPHQTDSWIGSAYVDLSRLGEKSPRTLTISGVYPLFGRNASDLSGAALRIHV
  LLSPLSPHTEPARELDSMDCSSHSESEQHPRKSDALQLSPPHVLQTSPTSTQVHGNSAAAQVCPAQEGPP
  ELAGTFAVSILVERAMHLSLKGSPLTDRKVSVPSCCVSFATATELSPVYTHVVENTDSPIWGFHQQARLS
  KELLLDPHQTLVFKVWHKGDEERVVGFASVDLSPLLSGFQFICGWYNITDFSGECQGQIKVAISPMESLM
  HLKEERQARRGIDTPGALIPLFSALSFPASAGCDAFPRPIARHVEGQLAHTSPKEDGLSSPARNGAIRSQ
  AARHEEHVQNIRRFHESLQHGEAVLTSDEKLTTAPSSSHTSILTSLRKNLSELDEIQKYFSQKLSKPFLP
  FSSQSSPAVSQSQESQRDPVAAGTGRQDPENQCILEKSNHLVSQVSSLISDLQTLTRGSQAALTSQQARS
  RSRAVTTIPDAQGTEAAGEGSTTLEEPLAGAIEASTDSLPPPVEEPSKGGGMLHESLEQTMPITRVQSID
  DTEVGPDYSDEDYEEDIIEPRTLNEITTVTDRTSPWSSFMSDMSEVLSPQPTEVQREGPSCPPEPFPREE
  LKVKSSPQKAVSPQPAQGSPSQSGVCEGGAYKIEVEDLASAKPQPVPSLTFSEAQEGSDSVGWRASQINQ
  VRKPMPEMLAESEAFSSEFSDSSESFETFPLHLPSQSKREDYKDSPAVRQKQVPTGSEVSTRQTLLLPEP
  VVVPNFFLPPQQLEASLRMISHSPGLPPAATTDQDKSEATRGALAQRPCRPRPYSIPPNLPEEETRRIAR
  IFSSQYSKKTEET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017985
• ORF Size: 6969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017985.2, NP_001017985.2
• RefSeq Size: 7813 bp
• RefSeq ORF: 6972 bp
• Locus ID: 277939
• Cytogenetics: 7 E2