Pigw (NM_027388) Mouse Tagged ORF Clone

CAT#: MG216866

Pigw (GFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis class W (Pigw) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pigw
• Synonyms: 2610044A17Rik; Gwt1; PIG-W
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216866 representing NM_027388
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAAAAGCAGCTGAAGGAAGCCTTTGTCAGGAACCTCAGTGGGACCAGTGTGCTGGAAGTGACCC
  AGGGCCTGTGCTTTCCTGCCTTCTGTATCCTGTGTAGAGGCCTTTGGATCATCTTCTCACAGCACGTATG
  CTCTTTCTCAAACACCTGGAGCACACGATTCCTCATGGACTTTGTTGTCTTGATAGTCCCCCTAGTGATC
  ACTCTGACTGTATTGTCTTCCTTTATCCTCCTCGAGAATCTCACTGTGATTGTCTGGGGGGCATGGCTGT
  TGTATCAGATATACCACAGGAGGACTTGCTACGCCAAAGTGCCTGTCCAGAAAGTCTTTGCAAACTTCTT
  GAAAATCAGCCTAGAATCTGAGTACAATCCCGCAATCACCTGTTACCGGGTCATTAACAGCGTATTTACG
  GCTATTGCCATTTTGGCCGTGGATTTCCCACTTTTCCCTAGAAGATTTGCCAAAACTGAACTTTATGGGA
  CAGGAGCAATGGATTTTGGAGTAGGTGGCTTTATTTTTGGGGCGGCAATGGTTTGTCCAGAGGTTAGAAG
  GAAATCTATAGAAGAGTCTAGATTTAATTATCTTAGAAAATCGCTGTATTCTGTCTGGCCATTGGTCTTC
  TTAGGAATGGGACGGTTGGTCATTATAAAGTCCATAGGCTACCAGGAACATTCTACCGAGTACGGCATTC
  ACTGGAACTTTTTCTTTACCATCATAGTTGTGCGATTAGTAACGTCACTGCTTTTGATTATTTTTCCTTT
  AAATAAGTCGTGGATTGTGGCTGTCAGCATAACTGTGGTATACCAGCTAGCCCTGGATTATACTCCACTC
  AAGAGGATACTTTTGTATGGCACTGATGGCAGTGGCACAAGGGTTGGCTTCTTAAATGCTAACCGAGAGG
  GAATAATTTCCACTTTGGGGTATGTGACAATACACATGGCTGGTGTACAAACGGGATTGTATGTGCTTAA
  GGGAAGAGCACAAGTCAGAGACTGGATAAAAGCTACTTGTTGGGTTTTCTCAGTAGCTGTTGGCTTCTTC
  ATTTCTCTTCATATAGTGCAAGTAAACATAGAAGCAGTATCTCGAAGAATGGCCAATTTAGCCTTTTGTC
  TGTGGGTAGTTGCTTCCAGTCTGATGCTTCTTAGTTGTCTGTTACTGAGTGGTATAATTTTGAGTTTTGC
  CCAATTTCTAATTAAAGGGTCTCTAGTTCCATGTTCTTGGAAACTTATACAGTCTCCTACTACACATAAA
  AACCATTCTGAATCTCTAATCTTAGAAGCTGAAAAAAACCAACCCAGTCTTTGTTTGATCACAGCTCTGA
  ACAGAAACCAGCTGTTTTTTTTCTTACTGTCAAATATAACAACTGGTCTGATTAACCTGACGATGGATAC
  ATTACACACTGGTGCCTTGTGGACCTTAGTTGTGCTCAGTATTTATATGTTTACCAACTGTTTAGTTATA
  TATGTACTTGATTTACAAGGTAAAACCATAAAGTTTTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216866 representing NM_027388
  Red=Cloning site Green=Tags(s)
  
  MSQKQLKEAFVRNLSGTSVLEVTQGLCFPAFCILCRGLWIIFSQHVCSFSNTWSTRFLMDFVVLIVPLVI
  TLTVLSSFILLENLTVIVWGAWLLYQIYHRRTCYAKVPVQKVFANFLKISLESEYNPAITCYRVINSVFT
  AIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFIFGAAMVCPEVRRKSIEESRFNYLRKSLYSVWPLVF
  LGMGRLVIIKSIGYQEHSTEYGIHWNFFFTIIVVRLVTSLLLIIFPLNKSWIVAVSITVVYQLALDYTPL
  KRILLYGTDGSGTRVGFLNANREGIISTLGYVTIHMAGVQTGLYVLKGRAQVRDWIKATCWVFSVAVGFF
  ISLHIVQVNIEAVSRRMANLAFCLWVVASSLMLLSCLLLSGIILSFAQFLIKGSLVPCSWKLIQSPTTHK
  NHSESLILEAEKNQPSLCLITALNRNQLFFFLLSNITTGLINLTMDTLHTGALWTLVVLSIYMFTNCLVI
  YVLDLQGKTIKFW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027388
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027388.2, NP_081664.2
• RefSeq Size: 2677
• RefSeq ORF: 1512
• Locus ID: 70325