Smurf2 (NM_025481) Mouse Tagged ORF Clone

CAT#: MG216928

Smurf2 (GFP-tagged) - Mouse SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smurf2
• Synonyms: 2810411E22Rik; AI558114; AI649275
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216928 representing NM_025481
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAACCCCGGAGGCCGGAGGAACGGGCCCGTCAAGCTGCGCCTGACAGTACTCTGTGCAAAAAACT
  TGGTGAAAAAGGATTTTTTCCGTCTTCCTGATCCATTTGCTAAGGTGGTAGTTGATGGTTCTGGGCAGTG
  CCATTCTACAGATACTGTGAAGAACACACTTGATCCAAAGTGGAATCAGCATTATGACCTGTATATCGGA
  AAGTCTGATTCAGTTACGATCAGCGTATGGAATCATAAGAAGATCCATAAGAAGCAAGGTGCTGGATTTC
  TTGGTTGTGTTCGTCTTCTTTCCAATGCCATCAACCGCCTCAAGGACACTGGTTATCAGAGGTTGGATTT
  ATGCAAACTGGGGCCAAATGACAATGATACAGTTCGAGGACAAATTGTAGTAAGTCTTCAGTCCAGAGAC
  CGAATAGGCACGGGAGGACAAGTTGTGGACTGTAGTCGTTTGTTTGACAATGATTTGCCAGATGGTTGGG
  AAGAAAGGCGGACTGCCTCGGGAAGAATCCAGTATCTAAACCACATCACAAGAACTACACAGTGGGAACG
  CCCAACACGACCGGCGTCAGAATATTCTAGTCCTGGCAGACCCCTCAGCTGCTTTGTGGATGAGAATACT
  CCAATTACTGGAACAAATGGTGCAACGTGTGGACATTCTTCAGACCCCAGACTAGCAGAGAGAAGAGTCA
  GGTCCCAGCGACATAGAAATTACATGAGCAGGACACACTTACACACTCCTCCAGACCTACCGGAAGGCTA
  TGAACAAAGGACAACGCAGCAGGGTCAGGTATATTTCTTACATACTCAGACTGGTGTGAGCACATGGCAT
  GATCCACGAGTGCCTAGGGATCTTAGCAACATCAATTGTGAAGAGCTCGGTCCTTTGCCTCCTGGATGGG
  AGATCCGCAATACCGCAACAGGAAGAGTTTATTTCGTTGACCATAACAACAGAACAACACAATTTACAGA
  TCCCCGGCTCTCTGCTAACTTGCATTTAGTTTTAAATCGTCAGAACCAGTTGAAAGACCAACAGCAACAG
  CAAGTGGTGCCATTGTGTCCCGATGACACTGAGTGTCTGACAGTGCCAAGATACAAGCGAGATTTGGTTC
  AAAAACTAAAAATCTTGCGGCAAGAACTTTCCCAACAGCAGCCTCAAGCTGGCCACTGCCGTATTGAGGT
  GTCTAGGGAAGAGATTTTTGAGGAATCATATAGACAGGTCATGAAAATGAGGCCAAAAGATCTATGGAAG
  CGATTAATGATAAAATTTCGTGGAGAAGAAGGTCTTGACTATGGAGGAGTTGCCAGGGAATGGTTGTATC
  TCCTGTCACATGAAATGTTGAATCCTTACTATGGTCTCTTCCAGTATTCTAGAGATGATATCTACACATT
  GCAGATCAATCCTGATTCTGCAGTTAATCCGGAACATTTATCCTATTTCCACTTTGTTGGACGGATAATG
  GGAATGGCTGTATTCCATGGACATTATATTGACGGTGGCTTCACATTACCTTTTTATAAACAGTTGCTTG
  GGAAGTCAATTACTTTGGATGACATGGAGCTAGTTGACCCAGACCTTCATAATAGTTTAGTGTGGATACT
  TGAAAACGATATCACAGGTGTTCTGGACCATACCTTCTGTGTTGAGCATAATGCATATGGTGAAATAATC
  CAGCATGAACTCAAACCAAATGGCAAAAGTATCCCTGTTACTGAAGAAAATAAAAAAGAATATGTCAGGC
  TTTATGTCAACTGGAGATTTCTCCGAGGCATCGAAGCTCAGTTCCTGGCGCTGCAGAAGGGCTTTAATGA
  AGTCATTCCCCAGCACCTGCTGAAGACATTTGATGAGAAGGAGCTGGAGCTCATTATTTGTGGACTTGGC
  AAAATTGATGTGAGTGACTGGAAGGTCAACACCCGTTTAAAACACTGTACCCCAGACAGCAATGTCGTCA
  AGTGGTTCTGGAAAGCGGTGGAGTTTTTTGATGAAGAGAGACGAGCACGGTTGCTCCAGTTTGTGACAGG
  GTCCTCTCGAGTGCCTCTGCAGGGCTTCAAAGCACTACAAGGTGCTGCAGGCCCACGGCTCTTTACCATA
  CACCAGATTGATGCCTGCACGAACAACTTGCCAAAAGCCCACACTTGCTTCAATCGAATAGACATTCCAC
  CCTATGAAAGCTATGAGAAGCTCTATGAAAAGCTGCTAACGGCCATCGAGGAGACATGTGGCTTTGCTGT
  GGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216928 representing NM_025481
  Red=Cloning site Green=Tags(s)
  
  MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIG
  KSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRD
  RIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENT
  PITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWH
  DPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQ
  QVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWK
  RLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIM
  GMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEII
  QHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLG
  KIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTI
  HQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025481
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025481.2, NP_079757.2
• RefSeq Size: 5346
• RefSeq ORF: 2247
• Locus ID: 66313