Rab11fip5 (NM_001003955) Mouse Tagged ORF Clone

CAT#: MG216985

Rab11fip5 (GFP-tagged) - Mouse RAB11 family interacting protein 5 (class I) (Rab11fip5) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rab11fip5
• Synonyms: 9130206P09Rik; C75969; D6Ertd32e; GAF1; RIP11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216985 representing NM_001003955
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGTGCGGGACCCTGAGCCGGCGGCCGGATCCTCCCGCTGGCTGCCCACGCATGTCCAGGTGA
  CCGTGCTGCGGGCCAGCGGGTTGAGGGGCAAGAGCTCGGGCGCCGGCAGCACCAGCGACGCGTACACGGT
  GATCCAGGTGGGCCGAGAGAAGTACAGCACCTCGGTAGTGGAGAAGACGCAAGGCTGCCCGGAATGGTGC
  GAAGAGTGTTCGTTCGAGCTACCGCCTGGAGCCCTGGACGGCCTGCTGCGGGCACAGGAGGCAGATGCGG
  GCCCTGCGCCCTGGGCCTCCGGCCCCAACGCCGCCTGCGAGCTCGTGCTCACCACCATGCATCGCTCACT
  CATCGGCGTGGACAAGTTTCTGGGCCGGGCAACCGTAGCTCTGGACGAGGTCTTCCGCGCAGGCCGCGCC
  CAGCACACGCAGTGGTACAGGCTTCACTCCAAGCCAGGCAAGAAGGAGAAGGAGCGCGGTGAGATCCAGG
  TGACCATCCAGTTCACACGGAACAACCTGAGCGCCAGCATGTTTGATCTTTCCATGAAGGACAAGCCACG
  GTCTCCCTTCAGCAAGCTCAAGGACAGAGTGAAGGGCAAGAAGAAGTATGATCTCGAATCTGCCTCAGCG
  ATTCTTCCAAGCAGCGCCCTGGAGGATCCTGAGTTAGGCAGCCTGGGCAAGATGGGCAAAGCCAAAGGTT
  TCTTCCTCCGAAACAAGCTACGCAAGTCCTCGCTCACACAGTCTAACACCTCTCTGGGCTCTGACAGCAC
  CTTGTCTTCCACTAGTGGCAGCCTGGTCTACCAGGGACCCGGGGCGGAGCTCCTCACCCGCTCACCAAGC
  CACAGCAGCTGGCTGTCCACTGAAGGGGGTAGGGACTCTATACAGTCCCCCAAGCTGCTCACCCACAAGA
  GGACATACAGTGATGAGGCCAGCCAGCTTCGAGCAGCTCCTCCCCGGGCCCTTCTTGAGCTCCAAGGCCA
  CCTTGATGGTGCCTCCCGCTCTTCCCTCTGCGTCAATGGGAGCCATGTGTACAATGAGGAACCTCAGCCC
  CCCTTACGGCACCGCAGCTCCATCTCAGGCCCCTTTCCACCTTCAAGCTCCTTACACAGTGTCCCACCTC
  GGTCCTCTGAGGAGGGGTCTCGGTCCTCGGATGATTCTTGGGGCAGAGGCAGCCATGGCACCAGCAGCTC
  AGAGGCAGTGCCTGGACAGGAGGAGCTGAGCAAGCAGGCCAAAGGGGCCAGTTGCTCTGGAGAGGAGGAG
  GGGGCCCGGTTACCCGAGGGGAAGCCAGTCCAGGTTGCCACCCCCATGGTGGCCTCCTCTGAGGCTGTGG
  CAGCAGAAAAGGATCGGAAGCCCCGAATGGGGCTCTTCCATCACCACCACCACCAAGGGCTGAGTCGGAG
  TGAGCAGGGGCGCCGTGGCTCAGTCGGGGAGAAGGGGAGTCCGTCCCTGGGAGCCTCCCCCCACCATTCA
  TCCACCGGGGAGGAAAAGGCCAAGAGTAGCTGGTTTGGCCTGAGAGAATCCAAGGAGCCAACTCAGAAGC
  CCAGCCTGGACGTGTCTCCTCAGGTAGAATCTGACCCAGCTGCTCCTCACCCCTGCTCCCCCCAGGCTCT
  GGCCCCCCCTCCTGCTCCCGCTGCTGCTCCCATGCTAAGCACTAACCTTTTTGCAGTCACCTCCCCTGCC
  GCTGCCACTGCCGCTGCTGCCACCATTGTCCTTGAAGCCACCCCATCCGGATTCTTGGGGGTCACCAACC
  CGTTCCTCAACTCCTTGCAGAGCAACCCCTTCTTCGAGGATCTCAAAGCCGACATAGCACTAAATTCTCC
  TTCACCTGCTCCCTCTCTCCCCAGTGCCTCGAGGGCCAGCCTGGCCCCCCTGGCCTCCCCTGGGAAAGCC
  CTGCCTGAGTGGGACGACACCTTCAACATCTTTGCTGCTGGCAGGCTGCAGCAAGAGGCCGGGAGTGGGA
  TCCTGGCCCCTGCAGGAATGGGGCTGGAGGAGGATGGGCTGCAAGACCCAGGCCCTAGGACCATGGCAGT
  GAAAGCGACAGAGCCCCAGGGAGAGCCTGGGAGAGGAAGAAGAGGAAGCAACATATGGCTAGAGCCCAAG
  GTTTCTGTGGACTCAGAACTGGACCAGCCAAGCACAAGTATGTCTGACCCAGGACCCTTTGGGTCTTCAG
  GGAGTGGTCCATCCTCCAGGGCATCCCAGTTGCACCTGCAAGCCTCAGCCTCAACACCAGACCGGGAGCT
  TCCTGCCTCAGAAGGGGGAGCGGGGCAGAGTCCAGCAGATAGTGGGGCATCTCTGTTCAGCTCCCCAGAA
  GTTCTCAGTGTGTGGGAGAGATTTCCTGGTTCAGATGACACTCCTGGAGGCCGAGATGAGGAGGCCCCCC
  AAGATGGAAGCCAGCTTTTCCAGGAGCTGAACACAGTGGAGGATTCCTGGCCTTGGGATGTGATCACCAT
  TTCTCCTACAGCAGAGGTCGCTTCACCTGTCATGAAGGGAGAATCCGACGGGGTACTTTCTTCCCAGGTG
  CAGCCTGAATCTCCAGATACTGTGCCCCCCATGGGGAGCGAAGGGCTCCCAGCATTGCTGGAGCCAGAGC
  CAGAGCAGGGATTGGATGAGGGGCCATGGCTTGGCCCTCCACCTCCCAAGCCACCACGCCTCTTCACACC
  CACCAATTCTCAGGTGGAGGAGGAGGACAAAGAGGAGGAAGAGAATGCTACAGGGCGGCAAAGTAGCAGG
  GGACCAGGGGTAGAAAAAGACAGCACCCCCCGTGCATTGGCCATTGGTCCCCAAGAGTCTAAGGAGGAAT
  GGGTAAATCCAGAGTTGGAGAAATTGCATAGACTGCCCTCTGGAACCCTGATAGGTAAGCCAGAACTTGA
  AGACCCAGTAGGAGAAACTAGTAGCCCTGTGTTTGGGGACTGTCCACCTAGGCCCACCAGCTGCCCTGAG
  GGGCCTGTCCCCAGACCCCATAACTCAATCAGCTCCACTCTGCTGAGTCAGAAGGTCTTGGGGACCTCAG
  AGACAGAGGAGGGCTTTGAAACCAAGAGCCAGGAGCTAGCCAAAGAGGGGTTTGGGCCTCTCTCAGTCCC
  CTCCCAGCAGTCAAGTATGCGGGCCAAGGAAGAGGAGGAGGGGGAGGAAGAGGAGGAAGAGGGAGAGGAG
  GAGGAGGCATTGGAGACCTCCAATTCCTTCTTATGTCAGGAAAGCCAAGATCCCCCCAGCTTCCCATCCA
  TATCACCACCAGGGTCAAGGGGATCCTCTATTCACTCTGGTCCAGAAGAGTTGCCCACACCCCCAGAACC
  TGCCTTCCCACCTCCCCCTCTCCCACCCTGGGCCAGCCACCGCCACGGCGTGCCAGGCCCTCCATGCCCT
  CCCCTGCCTATAGCCTGGCCCTTGACCTCTTCTTCCTCACCTCCCGAGGAGTCAGCTTCACCCCTCGGTC
  CCCCTGAGCTCTCCCCCACTGGGGGCTCCCCTACATCATATGGGGAAGACCATGCTGCAGCCACCCCAGC
  CTCCCCGCTTGTGCTTCTGCCCCTGGAGACACGACCAGCTGAGGAGCCCCAGCCCAGCGGCAGTCCCCAC
  CCTGTGAAACCCCTTACTGCTGCCCCTGTGGAGGCTAGCCCCGACAGGAAACAGCCCCGTACCAGCCTGA
  GCACAGCCCTGAGTAGCGGACTGGAGAGGCTCAAAACTGTCACATCTGGTGGCATCCAGTCTGTGCTTCC
  AGCGTCCCAGCTTGGCTCAAGTGTGGACACCAAGAGGCCAAAGGATTCTGCTGTGCTGGACCAGTCGGCC
  AAGTACTATCACCTGACTCATGATGAGCTCATTGGCCTGCTCCTGCAGCGTGAGCGTGAGCTGAGCCAGA
  GAGACGAGCATGTGCAAGAACTGGAGAGCTACATTGACCGGCTGCTGGTGCGGATCATGGAGACCTCGCC
  CACACTGCTGCAAATCTCCCCTGGCCCCCCCAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216985 representing NM_001003955
  Red=Cloning site Green=Tags(s)
  
  MALVRDPEPAAGSSRWLPTHVQVTVLRASGLRGKSSGAGSTSDAYTVIQVGREKYSTSVVEKTQGCPEWC
  EECSFELPPGALDGLLRAQEADAGPAPWASGPNAACELVLTTMHRSLIGVDKFLGRATVALDEVFRAGRA
  QHTQWYRLHSKPGKKEKERGEIQVTIQFTRNNLSASMFDLSMKDKPRSPFSKLKDRVKGKKKYDLESASA
  ILPSSALEDPELGSLGKMGKAKGFFLRNKLRKSSLTQSNTSLGSDSTLSSTSGSLVYQGPGAELLTRSPS
  HSSWLSTEGGRDSIQSPKLLTHKRTYSDEASQLRAAPPRALLELQGHLDGASRSSLCVNGSHVYNEEPQP
  PLRHRSSISGPFPPSSSLHSVPPRSSEEGSRSSDDSWGRGSHGTSSSEAVPGQEELSKQAKGASCSGEEE
  GARLPEGKPVQVATPMVASSEAVAAEKDRKPRMGLFHHHHHQGLSRSEQGRRGSVGEKGSPSLGASPHHS
  STGEEKAKSSWFGLRESKEPTQKPSLDVSPQVESDPAAPHPCSPQALAPPPAPAAAPMLSTNLFAVTSPA
  AATAAAATIVLEATPSGFLGVTNPFLNSLQSNPFFEDLKADIALNSPSPAPSLPSASRASLAPLASPGKA
  LPEWDDTFNIFAAGRLQQEAGSGILAPAGMGLEEDGLQDPGPRTMAVKATEPQGEPGRGRRGSNIWLEPK
  VSVDSELDQPSTSMSDPGPFGSSGSGPSSRASQLHLQASASTPDRELPASEGGAGQSPADSGASLFSSPE
  VLSVWERFPGSDDTPGGRDEEAPQDGSQLFQELNTVEDSWPWDVITISPTAEVASPVMKGESDGVLSSQV
  QPESPDTVPPMGSEGLPALLEPEPEQGLDEGPWLGPPPPKPPRLFTPTNSQVEEEDKEEEENATGRQSSR
  GPGVEKDSTPRALAIGPQESKEEWVNPELEKLHRLPSGTLIGKPELEDPVGETSSPVFGDCPPRPTSCPE
  GPVPRPHNSISSTLLSQKVLGTSETEEGFETKSQELAKEGFGPLSVPSQQSSMRAKEEEEGEEEEEEGEE
  EEALETSNSFLCQESQDPPSFPSISPPGSRGSSIHSGPEELPTPPEPAFPPPPLPPWASHRHGVPGPPCP
  PLPIAWPLTSSSSPPEESASPLGPPELSPTGGSPTSYGEDHAAATPASPLVLLPLETRPAEEPQPSGSPH
  PVKPLTAAPVEASPDRKQPRTSLSTALSSGLERLKTVTSGGIQSVLPASQLGSSVDTKRPKDSAVLDQSA
  KYYHLTHDELIGLLLQRERELSQRDEHVQELESYIDRLLVRIMETSPTLLQISPGPPK
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003955
• ORF Size: 3954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003955.2, NP_001003955.1
• RefSeq Size: 6156 bp
• RefSeq ORF: 3957 bp
• Locus ID: 52055
• Cytogenetics: 6 37.35 cM