Spire1 (NM_194355) Mouse Tagged ORF Clone

CAT#: MG217387

Spire1 (GFP-tagged) - Mouse spire homolog 1 (Drosophila) (Spire1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spire1
• Synonyms: 6030430B19Rik; AI415299; AU022898; AW550622; Spir-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217387 representing NM_194355
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAATACCGTGGAGGCTGACGGGAGCAAGGATGAAGGGTACGAGGCTGCAGATGAAGGCCCGGAAG
  ATGAAGACGGAGAGAAGAGAAGCATCTCAGCCATCCGGTCCTATCAGGACGTTATGAAGATCTGTGCTGC
  TCACCTCCCAACTGAGTCGGAGGCACCCAATCATTATCAGGCAGTATGTCGGGCCCTGTTCGCAGAAACC
  ATGGAACTGCATACATTTCTGACCAAAATTAAGAGTGCAAAGGAGAACCTTAAGAAGATTCAAGAAATGG
  AAAAGGGTGATGAATCTAGCACAGATCTGGAGGACCTGAAAAATGCAGACTGGGCCCGGTTCTGGGTACA
  AGTGATGAGGGATTTGCGAAATGGGGTAAAACTTAAGAAAGTCCAGCAGCGGCAGTACAACCCTCTGCCC
  ATTGAGTACCAACTGACCCCTTATGAGATGCTCATGGACGACATTCGGTGCAAAAGATACACCTTGAGAA
  AAGTAATGGTAAATGGTGACGTCCCCCCTCGGTTGAAAAAGAGTGCTCATGAGGTCATCCTTGACTTTAT
  CAGATCAAGACCCCCTTTAAATCCAGTTTCAGCCAGAAAACTGAAACCAACCCCACCACGGCCACGGAGC
  CTCCATGAAAGAATATTAGAAGAAATTAAAGCAGAAAGAAAGCTTCGACCTGTGTCACCAGAAGAAATTA
  GACGGAGCAGACTAGATGTCACTACGCCAGAATCTCCAAAGAATGTTGGAGAATCATCTATGGTGAATGG
  AGGCTTAACATCTCAAACAAAAGAAAATGGGCTGAGCGCTGCCCAGCAGGGGTCTGCGCAGAGGAAGAGG
  CTCCTCAAAGCCCCAACCCTGGCTGAACTGGACAGCTCTGACTCTGAGGAGGAAAAAAGTCTGCACAAGT
  CAACCAGCAGCAGCAGTGCGTCTCCCTCCCTCTATGAGGATCCAGTACTGGAGGCCATGTGCTCCAGGAA
  GAAGCCTCCAAAATTCTTGCCCATCTCATCAACACCCCAGCCAGAGAGAAGGCAGCCACCGCAGAGGCGC
  CACTCCATTGAGAAGGAAACACCTACGAACGTGAGGCAGTTTCTGCCACCATCCAGGCAGAGCTCACGGT
  CTCTTGTTCCTAGGATAACCGGTGTATGGCCCAGGACGCCTTTCCGACCACTTTTCTCTACCATACAAAC
  AGCTTCTCTGCTGAGCTCTCATCCTTTTGAAGCAGCCATGTTTGGGGTAGCAGGGGCTATGTATTATCTG
  TTTGAGAGAGCTTTTACCAGCAGGTGGAAACCCTCAAAGGAGGAATTCTGCTACCCAGTGGAGTGCCTCG
  CTCTCACTGTGGAGGAAGTGATGCACATCCGTCAGGTCCTGGTGAAAGCAGAGCTGGAAAAGTACCAACA
  GTATAAGGATGTCTACACTGCCCTGAAAAAAGGAAAGCTCTGCTTCTGTTGCCGAACCCGGAGGTTTTCC
  TTCTTCACCTGGTCTTACACCTGTCAGTTCTGTAAGAGGCCCGTGTGCTCACAGTGTTGCAAAAAGATGC
  GGCTGCCTTCCAAGCCATACTCTACTCTACCTATCTTCTCTTTGGGACCTTCTGCCTTGCAAAGGGGGGA
  AAGCTGTTCGAGGTCAGAAAAACCCTCCACTAGTCACCATCGGCCTCTTCGAAGTATTGCCAGGTTCTCC
  ACAAAATCCAGGTCTGTGGACAAGTCCGATGAGGAGCTCCAGTTTCCCAAAGAGCTCATGGAGGACTGGA
  GCACGATGGAGGTGTGTGTGGACTGCAAAAAGTTCATCTCCGAGATCATCAGCTCAAGCCGGCGCAGCCT
  GGTGCTGGCTAACAAAAGAGCCAGGTTGAAAAGGAAGACACAGTCTTTCTACATGTCCTCGGCGGGCCCC
  TCGGAGTACTGCCCTTCAGAACGGACTATCAACGAGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217387 representing NM_194355
  Red=Cloning site Green=Tags(s)
  
  MANTVEADGSKDEGYEAADEGPEDEDGEKRSISAIRSYQDVMKICAAHLPTESEAPNHYQAVCRALFAET
  MELHTFLTKIKSAKENLKKIQEMEKGDESSTDLEDLKNADWARFWVQVMRDLRNGVKLKKVQQRQYNPLP
  IEYQLTPYEMLMDDIRCKRYTLRKVMVNGDVPPRLKKSAHEVILDFIRSRPPLNPVSARKLKPTPPRPRS
  LHERILEEIKAERKLRPVSPEEIRRSRLDVTTPESPKNVGESSMVNGGLTSQTKENGLSAAQQGSAQRKR
  LLKAPTLAELDSSDSEEEKSLHKSTSSSSASPSLYEDPVLEAMCSRKKPPKFLPISSTPQPERRQPPQRR
  HSIEKETPTNVRQFLPPSRQSSRSLVPRITGVWPRTPFRPLFSTIQTASLLSSHPFEAAMFGVAGAMYYL
  FERAFTSRWKPSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFS
  FFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCSRSEKPSTSHHRPLRSIARFS
  TKSRSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSAGP
  SEYCPSERTINEI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194355
• ORF Size: 1929 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_194355.2, NP_919336.1
• RefSeq Size: 5033
• RefSeq ORF: 2367
• Locus ID: 68166