Ambra1 (NM_001080754) Mouse Tagged ORF Clone

CAT#: MG217481

Ambra1 (GFP-tagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ambra1
• Synonyms: 2310079H06Rik; A130023A14; AA474864; AV021921; D030051N19Rik; mKIAA1736
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217481 representing NM_001080754
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTTGTCCCAGAGAAGAATGCTGTACGAATCCTCTGGGGCCGAGAACGGGGCACTCGTGCCATGG
  GTGCCCAGCGGCTTCTGCAGGAGCTGGTGGAGGATAAGACTCGATGGATGAAATGGGAGGGCAAGAGAGT
  AGAACTGCCTGATAGTCCACGCTCGACCTTCTTACTGGCCTTCAGCCCAGACAGGACTCTCTTGGCATCC
  ACACATGTGAACCATAACATCTATATTACAGAGGTTAAGACTGGCAAGTGTGTTCACTCTCTGATCGGAC
  ACCGCCGTACTCCATGGTGTGTCACTTTTCACCCCACCATCTCAGGCCTTATTGCTTCTGGTTGCCTAGA
  TGGGGAAGTTAGGATTTGGGATTTACATGGGGGCAGTGAAAGCTGGTTCACAGACAGCAACAATGCCATA
  GCCTCCTTGGCTTTCCACCCTACGGCTCAGCTTCTACTAATTGCCACTGCAAATGAGATCCATTTCTGGG
  ATTGGAGTCGTCGGGAGCCCTTTGCTGTGGTGAAAACAGCCAGTGAGATGGAGCGTGTCCGCCTGGTGAG
  ATTTGATCCACTTGGACACTACTTACTTACAGCTATTGTTAACCCTTCTAATCAGCAGGGTGATGACGAA
  CCAGAAATCCCTATAGATGGAACAGAGTTATCTCATTACCGTCAACGTGCCCTCCTGCAATCACAGCCAG
  TTCGCCGGACACCTCTCCTCCACAATTTCCTGCACATGCTGTCCTCCCGTTCCTCTGGCATCCAGACCGA
  GCCCTTCCATCCCCCGGAGCAGGCTTCATCAACGCAGCAGGACCAGGGCCTCCTGAACCGGCCGTCTGCC
  TTCAGTACAGTCCAGAGCAGCACTGCGGGCAACACGCTCCGCAACCTCAGTCTGGGTCCTACCCGGCGCT
  CTTTGGGGGGCCCTTTGTCTAGCCACCCTTCTAGGTATCACAGAGAACTAGCCCCTGGGCTGACAGGTTC
  TGAGTGGACCCGGACAGTACTCACTCTGAACTCTCGTTCTGAGGTAGAATCTATGCCCCCACCGAGGACC
  AGTGCCTCCTCAGTGAGTTTGCTTTCTGTACTGAGACAGCAGGAAGGTGGCTCTCAGGCATCTGTGTATA
  CTTCAGCCACAGAAGGGAGGGGTTTTCCATCATCAGGGTTGGCAACTGAGTCAGATGGAGGGAATGGTTC
  CAGCCAAAACAACTCAGGCAGCATTCGCCATGAGCTTCAATGTGACCTGAGACGCTTCTTTTTGGAGTAT
  GACAGGCTGCAGGAGCTGGATCAGAGCCTGAGTGGGGAAACTCCCCAGACCCAACAGGCCCAGGAAATGC
  TCAACAATAATATTGAATCTGAGAGACCAGGTCCTTCCCACCTGCCTACCCCACACAGCAGTGAGAACAA
  CTCCAACCTGTCTCGGGGCCACCTTAATCGATGCCGTGCTTGCCATAATCTTCTAACCTTCAACAATGAT
  ACCCTTCGTTGGGAAAGAACCACACCTAACTACTCATCTGGTGAGGCTAGCTCTTCCTGGCACGTCTCCA
  CTACCTTTGAGGGCATGCCACCAAGTGGCAACCAGCTACCACCTCTTGAGCGGACTGAGGGCCAAATGCC
  TAGCTCCAGCAGGCTGGAGTTGAGCAGCTCTGCTAGTTCACAAGAGGAGAGGACTGTGGGGGTGGCCTTT
  AACCAGGAGACAGGCCACTGGGAACGCATTTATACCCAGTCCAGCAGATCTGGAACTGTATCACAGGAGG
  CCTTACATCAAGATATGCCTGAAGAAAGCTCTGAGGAAGACTCCCTCAGGAGGAGGCTGCTGGAGTCTTC
  CCTCATTTCATTATCCCGTTATGATGGAGCAGGATCCAGAGAGCACCCAATTTACCCAGACCCAGCGAGA
  TTATCTCCTGCTGCATACTACGCCCAGAGGATGATCCAGTATCTCTCAAGGAGAGACAGTATTCGCCAGC
  GCTCCATGCGCTACCAACAGAATCGGCTTCGTTCTTCCACCTCCTCTTCTTCCTCAGACAACCAGGGTCC
  ATCAGTAGAGGGAACCGACTTGGAATTTGAGGACTTTGAGGACAATGGTGACAGATCTAGGCACCGAGCT
  CCCCGAAATGCCCGAATGTCTGCACCTTCACTTGGACGATTTGTTCCAAGGCGTTTTTTGCTGCCTGAGT
  ACTTGCCTTATGCTGGGATTTTTCATGAACGTGGACAGCCTGGCTTGGCTACTCATTCTTCTGTTAACAG
  GGTCCTGGCAGGGGCAGTAATTGGAGATGGACAGTCTGCTGTGGCCAGTAACATTGCCAATACTACCTAC
  AGGCTCCAGTGGTGGGACTTCACTAAGTTTGACCTTCCAGAAATCAGTAATGCCTCTGTGAATGTCCTGG
  TACAGAATTGCAAGATCTACAATGATGCCAGCTGTGACATTTCTGCAGATGGTCAGCTCCTGGCAGCCTT
  CATCCCGAGCAGCCAGAGGGGCTTTCCTGATGAAGGCATCCTGGCAGTGTATTCCCTGGCCCCCCATAAC
  TTGGGGGAAATGCTCTACACCAAGCGATTTGGTCCCAATGCCATTTCTGTAAGCCTGTCCCCGATGGGCC
  GATACGTTATGGTGGGCTTGGCCTCACGAAGGATCCTACTGCACCCCTCCACAGAGCACATGGTAGCCCA
  GGTCTTCAGGCTGCAACAGGCCCATGGTGGTGAGACTTCCATGAGGAGAGTTTTCAATGTCCTTTACCCC
  ATGCCTGCAGACCAACGGAGACACGTCAGCATCAACTCTGCCCGTTGGCTACCCGAGCCAGGGCTTGGTT
  TGGCCTATGGCACCAACAAAGGAGACTTGGTGATCTGCCGACCAGAGGCCTTAAACTCTGGCATCGAGTA
  CTACTGGGACCAGCTAAGTGAAACAGTCTTCACTGTCCACTCAAGTAGCAGGAGCAGCGAGCGGCCTGGA
  ACCAGCAGAGCCACGTGGCGGACAGACAGAGACATGGGTTTGATGAATGCAATTGGGCTGCAGCCTCGGA
  ACCCAACCACCTCTGTGACATCTCAGGGCACCCAGACTCTGGCCCTCCAGTTGCAGAACGCTGAAACACA
  GACTGAGAGGGAGGAAGAGGAACCAGGGGCAGCATCTTCAGGTCCTGGTGAAGGCGAGGGCTCAGAATAT
  GGTGGCAGTGGGGAAGATGCCCTCAGCAGGATCCAGAGGCTGATGGCCGAGGGTGGCATGACAGCTGTGG
  TACAACGGGAACAGAGCACCACCATGGCCTCCATGGGTGGCTTTGGCAACAACATCATCGTTAGCCACCG
  CATTCACCGCAGCTCCCAGACAGGCACAGAGTCAGGGGCTGCTCGTACCTCCTCACCCCAGCCCTCCACC
  TCTCGAGGACTGCCATCAGAACCTGGGCAACTGGCAGAGCGAGCGCTAAGCCCCCGGACAGCCTCCTGGG
  ACCAGCCTAGTACCTCTGGGAGGGAACTACCCCAGCCAGCCCTGTCCTCTTCCTCCCCTGTCCCCATTCC
  AGTCCCCCTTGCCAGCAATGAGGGACCAACCATGCACTGCAATGTGACCAATAACAGTCACCTTCCTGAG
  GGTGATGGTAGCAACAGGGGGGAAGCTGCAGGCCCTAGTGGAGAGCCACAGAACCGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217481 representing NM_001080754
  Red=Cloning site Green=Tags(s)
  
  MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLAS
  THVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAI
  ASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDE
  PEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSA
  FSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRT
  SASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEY
  DRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNND
  TLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPSSSRLELSSSASSQEERTVGVAF
  NQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPAR
  LSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRA
  PRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTY
  RLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHN
  LGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYP
  MPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPG
  TSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEY
  GGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPST
  SRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPE
  GDGSNRGEAAGPSGEPQNR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080754
• ORF Size: 3627 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001080754.1, NP_001074223.1
• RefSeq Size: 5056
• RefSeq ORF: 3630
• Locus ID: 228361