Man2a1 (NM_008549) Mouse Tagged ORF Clone

CAT#: MG217804

Man2a1 (GFP-tagged) - Mouse mannosidase 2 alpha 1 (Man2a1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Man2a1
• Synonyms: Mana-2; Mana2; Map-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217804 representing NM_008549
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTAAGTCGCCAGTTCACCGTGTTTGGCAGCGCGATCTTCTGCGTCGTAATCTTCTCACTCTACC
  TGATGCTGGACAGGGGTCACTTGGACTACCCTCGGGGCCCGCGCCAGGAGGGCTCCTTTCCGCAGGGCCA
  GCTTTCAATATTGCAAGAAAAGATTGACCATTTGGAGCGTTTGCTCGCTGAGAACAACGAGATCATCTCA
  AATATCAGAGACTCAGTCATCAACCTGAGCGAGTCTGTGGAGGACGGCCCGCGGGGTTCACCAGGCAACG
  CCAGCCAAGGCTCCATCCACCTCCACTCGCCACAGTTGGCCCTGCAGGCTGACCCCAGAGACTGTTTGTT
  TGCTTCACAGAGTGGGAGTCAGCCCCGGGATGTGCAGATGTTGGATGTTTACGATCTGATTCCTTTTGAT
  AATCCAGATGGTGGAGTTTGGAAGCAAGGATTTGACATTAAGTATGAAGCGGATGAGTGGGACCATGAGC
  CCCTGCAAGTGTTTGTGGTGCCTCACTCCCATAATGACCCAGGTTGGTTGAAGACTTTCAATGACTACTT
  TAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCAAGCAGGAAGTTT
  ATGTGGTCTGAGATCTCTTACCTTGCAAAATGGTGGGATATTATAGATATTCCGAAGAAGGAAGCTGTTA
  AAAGTTTACTACAGAATGGTCAGCTGGAAATTGTGACCGGTGGCTGGGTTATGCCTGATGAAGCCACTCC
  ACATTATTTTGCCTTAATTGACCAACTAATTGAAGGGCACCAATGGCTGGAAAAAAATCTAGGAGTGAAA
  CCTCGATCGGGCTGGGCCATAGATCCCTTTGGTCATTCACCCACAATGGCTTATCTTCTAAAGCGTGCTG
  GATTTTCACACATGCTCATCCAGAGAGTCCATTATGCAATCAAAAAACACTTCTCTTTGCATAAAACGCT
  GGAGTTTTTCTGGAGACAGAATTGGGATCTTGGATCTGCTACAGACATTTTGTGCCATATGATGCCCTTC
  TACAGCTACGACATCCCTCACACCTGTGGGCCTGATCCTAAAATATGCTGCCAGTTTGATTTTAAACGGC
  TTCCTGGAGGCAGATATGGTTGTCCCTGGGGAGTTCCCCCAGAAGCAATATCTCCTGGAAATGTCCAAAG
  CAGGGCTCAGATGCTATTGGATCAGTACCGGAAAAAGTCAAAACTTTTCCGCACTAAAGTTCTGCTGGCT
  CCACTGGGAGACGACTTTCGGTTCAGTGAATACACAGAGTGGGATCTGCAGTGCAGGAACTACGAGCAAC
  TGTTCAGTTACATGAACTCGCAGCCTCATCTGAAAGTGAAGATCCAGTTTGGAACCTTGTCAGATTATTT
  CGACGCATTGGAGAAAGCGGTGGCAGCCGAGAAGAAGAGTAGCCAGTCTGTGTTCCCTGCCCTGAGTGGA
  GACTTCTTCACGTACGCTGACAGAGACGACCATTACTGGAGTGGCTACTTCACGTCCAGACCTTTCTACA
  AACGAATGGACAGAATAATGGAATCTCGTATAAGGGCTGCTGAAATTCTTTACCAGTTGGCCTTGAAACA
  AGCTCAGAAATACAAGATAAATAAATTTCTTTCATCACCTCATTACACAACACTGACAGAAGCCAGAAGG
  AACTTAGGACTATTTCAGCATCATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATG
  GTACCAGACTCTTTCAGTCATTAAATTCTTTGGAGAAGATAATTGGAGATTCTGCATTTCTTCTCATTTT
  AAAGGACAAAAAGCTGTACCAGTCAGATCCTTCCAAAGCCTTCTTAGAGATGGATACGAAGCAAAGTTCA
  CAAGATTCTCTGCCCCAAAAAATTATAATACAACTGAGCGCACAGGAGCCAAGGTACCTTGTGGTCTACA
  ATCCCTTTGAACAAGAACGGCATTCAGTGGTGTCCATCCGGGTAAACTCCGCCACAGTGAAAGTGCTGTC
  TGATTCGGGAAAACCGGTGGAGGTTCAAGTCAGTGCAGTTTGGAACGACATGAGGACAATTTCACAAGCA
  GCCTATGAGGTTTCTTTTCTAGCTCATATACCACCACTGGGACTGAAAGTGTTTAAGATCTTAGAGTCAC
  AAAGTTCAAGCTCACACTTGGCTGATTATGTCCTATATAATAATGATGGACTAGCAGAAAATGGAATATT
  CCACGTGAAGAACATGGTGGATGCTGGAGATGCCATAACAATAGAGAATCCCTTCCTGGCGATTTGGTTT
  GACCGATCTGGGCTGATGGAGAAAGTGAGAAGGAAAGAAGACAGTAGACAGCATGAACTGAAGGTCCAGT
  TCCTGTGGTACGGAACCACCAACAAAAGGGACAAGAGCGGTGCCTACCTCTTCCTGCCTGACGGGCAGGG
  CCAGCCATATGTTTCCCTAAGACCGCCCTTTGTCAGAGTGACACGTGGAAGGATCTACTCAGATGTGACC
  TGTTTCCTCGAACACGTTACTCACAAAGTCCGCCTGTACAACATTCAGGGAATAGAAGGTCAGTCCATGG
  AAGTTTCTAATATTGTAAACATCAGGAATGTGCATAACCGTGAGATTGTAATGAGAATTTCATCTAAAAT
  AAACAACCAAAATAGATATTATACTGACCTAAATGGATATCAGATTCAGCCTAGAAGGACCATGAGCAAA
  TTGCCTCTTCAAGCCAACGTTTACCCGATGTGCACAATGGCGTATATCCAGGATGCTGAGCACCGGCTCA
  CGCTGCTCTCTGCTCAGTCTCTAGGTGCTTCCAGCATGGCTTCTGGTCAGATTGAAGTCTTCATGGATCG
  AAGGCTCATGCAGGATGATAACCGTGGCCTTGGGCAAGGCGTCCATGACAATAAGATTACAGCTAATTTG
  TTTCGAATCCTCCTCGAGAAGAGAAGCGCTGTGAACATGGAAGAAGAAAAGAAGAGCCCTGTCAGCTACC
  CTTCCCTCCTCAGCCACATGACTTCGTCCTTCCTCAACCATCCCTTTCTCCCCATGGTACTAAGTGGCCA
  GCTCCCCTCCCCTGCCTTTGAGCTGCTGAGTGAATTTCCTCTGCTGCAGTCCTCTCTACCTTGTGATATC
  CATCTGGTCAACCTGCGGACAATACAATCAAAGATGGGCAAAGGCTATTCGGATGAGGCAGCCTTGATCC
  TCCACAGGAAAGGGTTTGATTGCCAGTTCTCCAGCAGAGGCATCGGGCTACCCTGTTCCACTACTCAGGG
  AAAGATGTCAGTTCTGAAACTTTTCAACAAGTTTGCTGTGGAGAGTCTCGTCCCTTCCTCTCTGTCCTTG
  ATGCACTCCCCTCCAGATGCCCAGAACATGAGTGAAGTCAGCCTGAGCCCCATGGAGATCAGCACGTTCC
  GTATCCGCTTGCGTTGGACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217804 representing NM_008549
  Red=Cloning site Green=Tags(s)
  
  MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRGPRQEGSFPQGQLSILQEKIDHLERLLAENNEIIS
  NIRDSVINLSESVEDGPRGSPGNASQGSIHLHSPQLALQADPRDCLFASQSGSQPRDVQMLDVYDLIPFD
  NPDGGVWKQGFDIKYEADEWDHEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSSRKF
  MWSEISYLAKWWDIIDIPKKEAVKSLLQNGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLEKNLGVK
  PRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPF
  YSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLA
  PLGDDFRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSG
  DFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARR
  NLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKIIGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSS
  QDSLPQKIIIQLSAQEPRYLVVYNPFEQERHSVVSIRVNSATVKVLSDSGKPVEVQVSAVWNDMRTISQA
  AYEVSFLAHIPPLGLKVFKILESQSSSSHLADYVLYNNDGLAENGIFHVKNMVDAGDAITIENPFLAIWF
  DRSGLMEKVRRKEDSRQHELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRGRIYSDVT
  CFLEHVTHKVRLYNIQGIEGQSMEVSNIVNIRNVHNREIVMRISSKINNQNRYYTDLNGYQIQPRRTMSK
  LPLQANVYPMCTMAYIQDAEHRLTLLSAQSLGASSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANL
  FRILLEKRSAVNMEEEKKSPVSYPSLLSHMTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDI
  HLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKMSVLKLFNKFAVESLVPSSLSL
  MHSPPDAQNMSEVSLSPMEISTFRIRLRWT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008549
• ORF Size: 3450 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008549.2, NP_032575.2
• RefSeq Size: 6091
• RefSeq ORF: 3453
• Locus ID: 17158
• Gene Summary: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway. [UniProtKB/Swiss-Prot Function]