Mxi1 (NM_001008542) Mouse Tagged ORF Clone

CAT#: MG218023

Mxi1 (GFP-tagged) - Mouse Max interacting protein 1 (Mxi1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mxi1
• Synonyms: bHLHc11; ENSMUSG00000067085; Gm10197; Mad2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218023 representing NM_001008542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCGGCCGCGGCCGCGCCCCAGCCCCCGGCGCAGCCGGAGGAGCCGGCGGGGGCCAAGCCCCGGT
  GCCCCTTCTCGGACATTTTCAACACCAGCGAGAACTCGATGGAGAAGCACATCAACACTTTTCTGCAGAA
  CGTGCAGATTCTGCTCGAGGCAGCCAGCTACCTGGAGCAGATCGAGAAAGAAAACAAAAAGTGTGAACAT
  GGCTACGCCTCATCGTTCCCCTCCATGCCGAGCCCCCGGCTACAGCACTCGAAGCCCCCACGGAGGTTGA
  GCCGGGCACAGAAACACAGCAGTGGAAGCAGCAACACCAGCACTGCCAACAGATCTACACACAATGAGTT
  GGAAAAGAACCGACGAGCTCACCTGCGCCTGTGTTTAGAACGCTTGAAAGTTCTGATCCCGCTGGGCCCA
  GACTGCACCAGGCACACAACACTCGGTTTGCTCAACAAAGCCAAAGCACACATCAAGAAACTTGAAGAAG
  CGGAGAGGAAGAGCCAGCACCAGCTAGAGAACTTGGAACGAGAACAGAGGTTTTTAAAGCGGCGACTGGA
  ACAGCTGCAGGGGCCTCAGGAGATGGAGCGGATACGAATGGACAGCATTGGATCAACCATCTCTTCAGAT
  CGCTCGGATTCAGAGCGAGAGGAGATTGAAGTGGATGTGGAAAGCACAGAGTTCTCCCATGGAGAAGCAG
  ACAGTGTCAGTACCACCAGCATCAGTGACCTTGACGACCACAGCAGCCTGCAGAGTGTCGGGAGTGACGA
  GGGTTATTCCAGTGCCAGTGTCAAACTCTCCTTCGCGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218023 representing NM_001008542
  Red=Cloning site Green=Tags(s)
  
  MPPAAAAPQPPAQPEEPAGAKPRCPFSDIFNTSENSMEKHINTFLQNVQILLEAASYLEQIEKENKKCEH
  GYASSFPSMPSPRLQHSKPPRRLSRAQKHSSGSSNTSTANRSTHNELEKNRRAHLRLCLERLKVLIPLGP
  DCTRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKRRLEQLQGPQEMERIRMDSIGSTISSD
  RSDSEREEIEVDVESTEFSHGEADSVSTTSISDLDDHSSLQSVGSDEGYSSASVKLSFAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008542
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008542.2, NP_001008542.2
• RefSeq Size: 5156 bp
• RefSeq ORF: 888 bp
• Locus ID: 17859
• Cytogenetics: 19 47.53 cM
• Gene Summary: This gene encodes a protein containing a helix-loop-helix domain characteristic of transcription factors, which allows heterodimerization and sequence-specific DNA binding. The encoded protein is related to a family of Myc/Max/Mad proteins that are involved in the regulation of several cellular processes. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate Myc function. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]