Tpm1 (NM_001164252) Mouse Tagged ORF Clone

CAT#: MG218444

Tpm1 (GFP-tagged) - Mouse tropomyosin 1 alpha (Tpm1) transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tpm1
• Synonyms: AA986836; AI854628; alpha-TM; TM2; Tm3; Tmpa; Tpm-1; TPM1kappa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218444 representing NM_001164252
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTAGCAGCTCGCTGGAGGCGGTGCGGAGAAAGATCCGGAGCCTGCAGGAGCAGGCGGATGCCG
  CGGAGGAGCGTGCGGGCAGCCTGCAGCGCGAGCTGGACCAGGAGCGGAAGCTGCGGGAAACCGCTGAAGC
  TGACGTAGCTTCTCTGAACAGACGCATCCAGCTGGTTGAGGAGGAGTTGGATCGTGCTCAGGAGCGTCTG
  GCCACAGCTCTGCAGAAGCTGGAGGAGGCCGAGAAGGCTGCAGATGAGAGTGAGAGAGGCATGAAAGTCA
  TTGAAAGCCGAGCCCAAAAAGATGAAGAAAAGATGGAGATTCAGGAGATCCAGCTGAAAGAGGCCAAGCA
  CATTGCTGAAGATGCTGACCGGAAGTATGAAGAGGTGGCCCGTAAGCTGGTCATCATCGAGAGCGACCTG
  GAACGTGCAGAGGAGCGGGCTGAGCTCTCAGAAGGCAAATGTGCCGAGCTTGAAGAAGAATTGAAAACGG
  TGACGAACAACTTGAAGTCACTGGAGGCTCAGGCTGAGAAGTACTCTCAGAAGGAAGACAAATATGAAGA
  GGAGATCAAGGTTCTCTCTGACAAGCTGAAGGAGGCTGAAACTCGGGCTGAGTTTGCAGAGAGATCAGTA
  ACCAAATTGGAGAAAAGCATTGATGACTTAGAAGAGAAAGTGGCCCATGCCAAAGAAGAAAACCTTAGTA
  TGCACCAGATGCTGGATCAGACTTTACTGGAGCTAAACAACATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218444 representing NM_001164252
  Red=Cloning site Green=Tags(s)
  
  MAGSSSLEAVRRKIRSLQEQADAAEERAGSLQRELDQERKLRETAEADVASLNRRIQLVEEELDRAQERL
  ATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDL
  ERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSV
  TKLEKSIDDLEEKVAHAKEENLSMHQMLDQTLLELNNM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164252
• ORF Size: 744 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164252.1, NP_001157724.1
• RefSeq Size: 1675
• RefSeq ORF: 747
• Locus ID: 22003
• Gene Summary: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. [UniProtKB/Swiss-Prot Function]