Prpf8 (NM_138659) Mouse Tagged ORF Clone

CAT#: MG218624

Prpf8 (GFP-tagged) - Mouse pre-mRNA processing factor 8 (Prpf8), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prpf8
• Synonyms: AU019467; D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218624 representing NM_138659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGAGTGTTTCCTTACCGAGGGCCGGGTAACCCGGTGCCCGGCCCTCTAGCCCCGCTGCCGGATT
  ATATGTCAGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAGTTGCAGGCCAAGCGATATGCAGA
  GAAGCGGAAATTTGGGTTTGTGGATGCTCAGAAGGAAGACATGCCACCAGAACATGTCAGGAAGATCATT
  CGAGACCATGGAGACATGACCAACAGGAAGTTCCGTCATGACAAAAGGGTCTATTTGGGAGCTCTTAAGT
  ACATGCCTCATGCAGTCCTCAAGCTCCTTGAGAACATGCCCATGCCTTGGGAGCAGATCCGGGACGTGCC
  TGTGCTGTACCACATCACTGGAGCCATCTCCTTTGTTAACGAGATTCCCTGGGTTATCGAGCCGGTTTAC
  ATCTCCCAGTGGGGGTCAATGTGGATCATGATGCGTAGAGAAAAAAGAGACAGGAGGCATTTCAAGAGGA
  TGCGTTTCCCCCCTTTCGATGACGAGGAACCGCCCCTGGATTATGCCGACAACATCTTAGATGTTGAGCC
  ACTGGAAGCCATTCAGCTAGAGCTGGACCCAGAGGAGGATGCCCCTGTGTTGGACTGGTTCTATGACCAC
  CAGCCTTTGAGAGACAGTAGGAAGTACGTTAATGGTTCCACCTACCAACGCTGGCAGTTCACATTGCCTA
  TGATGTCCACACTCTACCGACTGGCCAATCAGCTTCTGACTGACCTTGTAGATGACAACTACTTCTATCT
  CTTTGATCTGAAGGCCTTTTTTACCTCTAAGGCACTCAACATGGCCATTCCTGGGGGACCCAAATTTGAG
  CCTCTTGTTCGAGACATCAACTTACAGGATGAAGACTGGAATGAATTTAACGATATTAATAAGATCATCA
  TCCGTCAGCCTATTCGTACTGAGTACAAGATTGCTTTTCCTTACCTGTATAACAACCTTCCACACCACGT
  CCACCTGACCTGGTACCACACTCCCAACGTTGTGTTCATCAAGACTGAGGATCCTGATCTGCCAGCTTTC
  TACTTTGATCCTTTGATCAACCCAATCTCCCATAGACACTCAGTCAAGAGCCAAGAACCACTGCCTGATG
  ATGATGAAGAGTTTGAACTCCCAGAGTTTGTCGAGCCCTTCCTGAAGGATACACCTCTCTATACAGACAA
  CACAGCCAATGGCATTGCCCTGCTCTGGGCACCACGGCCCTTCAACCTACGCTCTGGTCGTACCCGCCGG
  GCCTTGGACATACCCCTTGTCAAGAACTGGTATCGGGAGCACTGTCCTGCTGGGCAGCCTGTGAAGGTTC
  GAGTCTCTTATCAGAAGCTGCTTAAGTACTATGTGCTGAATGCCCTCAAGCACCGGCCTCCCAAAGCCCA
  GAAGAAGAGGTATCTGTTTCGTTCTTTCAAAGCTACCAAATTCTTTCAGTCCACCAAGCTGGACTGGGTG
  GAGGTAGGGCTGCAGGTTTGCCGCCAGGGCTACAACATGCTCAACCTTCTCATTCACCGGAAGAACCTCA
  ACTATCTGCACTTGGACTACAACTTCAACCTGAAGCCTGTCAAGACGCTCACCACCAAGGAAAGAAAGAA
  ATCCCGTTTTGGGAATGCTTTCCATCTATGTCGGGAAGTACTGCGTTTAACCAAGCTGGTTGTGGACAGC
  CATGTGCAGTATCGCTTGGGCAATGTAGATGCCTTCCAGCTGGCAGATGGTTTGCAGTATATATTTGCCC
  ACGTGGGACAGTTGACAGGCATGTATCGATACAAATATAAGCTGATGCGGCAGATCCGCATGTGCAAGGA
  CTTGAAACATCTCATCTATTACCGCTTCAACACGGGTCCTGTAGGGAAAGGCCCTGGCTGTGGTTTCTGG
  GCTGCTGGCTGGCGGGTCTGGCTGTTCTTTATGCGTGGAATTACCCCTCTGCTAGAGCGATGGCTGGGCA
  ACCTTCTGGCCCGACAGTTTGAAGGCCGTCACTCAAAGGGGGTGGCAAAGACAGTAACGAAGCAGCGGGT
  GGAGTCGCACTTTGACCTGGAGCTCAGGGCTGCTGTGATGCACGACATTCTGGACATGATGCCTGAAGGC
  ATCAAGCAGAACAAGGCCCGGACAATTCTGCAGCACCTCAGTGAAGCCTGGCGCTGCTGGAAGGCCAACA
  TCCCTTGGAAGGTCCCAGGACTGCCAACACCTATAGAGAATATGATTCTTCGGTACGTTAAGGCCAAGGC
  TGATTGGTGGACCAATACTGCCCACTACAACCGGGAACGGATCCGTCGCGGGGCTACTGTAGACAAGACT
  GTTTGCAAAAAGAACCTGGGCCGCCTCACTCGCCTGTATCTGAAGGCAGAGCAGGAGCGGCAGCACAATT
  ACCTGAAGGATGGGCCTTACATCACAGCCGAGGAGGCAGTGGCCGTGTATACCACCACTGTGCACTGGCT
  GGAGAGCCGGAGATTCTCGCCCATCCCCTTCCCTCCACTCTCCTACAAGCATGATACCAAGCTGCTCATC
  TTGGCTCTGGAGCGTCTCAAGGAAGCTTATAGTGTGAAGTCTAGGTTAAACCAGTCTCAGAGGGAGGAGC
  TGGGTCTGATTGAACAAGCCTATGACAACCCCCATGAGGCTTTGTCCCGCATCAAGCGTCACCTCCTCAC
  ACAGAGAGCCTTTAAGGAAGTGGGCATTGAGTTTATGGATCTCTATAGCCACCTTGTACCAGTGTATGAT
  GTGGAACCACTGGAGAAAATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCAGATAAACGCC
  GTCTTTTCCCACCCTGGATCAAGCCTGCTGACACAGAGCCACCTCCACTGCTTGTTTACAAGTGGTGTCA
  AGGCATTAATAACCTGCAGGATGTGTGGGAGACGAGTGAGGGCGAGTGCAACGTCATGCTGGAGTCTCGG
  TTTGAGAAGATGTATGAGAAGATTGACCTGACCCTGCTTAACAGGCTGCTGCGACTCATTGTGGACCACA
  ACATAGCTGACTACATGACGGCCAAGAACAACGTAGTCATCAACTATAAGGACATGAACCATACAAACTC
  ATATGGAATCATCAGAGGCCTGCAGTTTGCCTCATTCATTGTACAGTACTATGGCTTGGTGATGGATTTG
  CTTGTACTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCTCCTCAGATGCCAAATGACTTTCTCAGTT
  TCCAGGACATAGCCACTGAGGCTGCACACCCAATCCGACTCTTCTGTAGATACATTGATCGCATCCATAT
  TTTCTTCAGGTTCACAGCAGATGAGGCTCGGGATCTGATCCAGCGTTACCTGACAGAACACCCAGACCCC
  AACAATGAAAACATTGTTGGCTACAATAACAAGAAATGCTGGCCCCGAGATGCCCGAATGCGGCTCATGA
  AGCATGATGTCAACTTGGGTCGGGCAGTGTTCTGGGACATCAAAAACCGGCTGCCACGATCGGTGACTAC
  AGTACAGTGGGAGAATAGCTTTGTGTCTGTGTACAGTAAAGACAACCCCAACCTGCTATTCAACATGTGT
  GGCTTTGAGTGCCGCATCCTGCCTAAGTGCCGTACCAGCTATGAAGAGTTCACCCACAAGGACGGGGTCT
  GGAACCTACAGAATGAGGTTACCAAGGAGCGGACAGCTCAGTGCTTCCTGCGTGTGGATGATGAGTCAAT
  GCAACGCTTCCATAACCGTGTGCGGCAGATTCTGATGGCCTCTGGCTCCACCACGTTCACCAAGATTGTG
  AATAAGTGGAACACAGCTCTCATCGGCCTTATGACATATTTTCGAGAGGCTGTGGTGAATACACAGGAAC
  TCCTGGACTTATTGGTGAAGTGTGAAAACAAGATCCAGACACGTATCAAGATTGGATTGAACTCCAAGAT
  GCCTAGCCGGTTCCCGCCTGTTGTATTCTACACCCCTAAGGAGCTGGGTGGGCTTGGCATGCTCTCGATG
  GGCCATGTGCTTATCCCCCAGTCTGACCTCAGGTGGTCCAAGCAGACAGATGTGGGCATCACACACTTCC
  GTTCAGGAATGAGTCATGAAGAAGACCAACTGATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAG
  CGAGTTCATTGACTCCCAGCGGGTCTGGGCTGAGTATGCACTCAAGAGACAAGAGGCTATTGCTCAGAAC
  AGACGCTTGACTTTAGAAGATTTAGAAGATTCATGGGATCGAGGCATTCCCCGGATCAATACCCTCTTCC
  AGAAAGACCGGCACACATTGGCTTATGATAAAGGCTGGCGTGTCCGAACTGACTTTAAGCAGTACCAGGT
  TTTGAAGCAGAACCCCTTTTGGTGGACACATCAGCGGCATGATGGCAAGCTTTGGAACCTCAATAACTAC
  CGCACAGACATGATTCAGGCGCTGGGTGGTGTGGAGGGCATTCTGGAGCACACTCTCTTCAAGGGCACTT
  ACTTCCCCACCTGGGAGGGTCTGTTCTGGGAGAAAGCCAGTGGCTTTGAGGAGTCCATGAAGTGGAAGAA
  GCTGACCAATGCTCAGCGATCAGGATTAAACCAGATTCCTAATCGTAGATTTACCCTCTGGTGGTCCCCA
  ACCATCAATCGAGCCAATGTCTATGTGGGCTTCCAGGTGCAGCTGGACCTGACAGGAATCTTCATGCACG
  GCAAGATCCCCACACTGAAGATCTCTCTCATCCAGATATTCCGAGCTCACTTGTGGCAGAAGATCCATGA
  GAGCATCGTGATGGACTTGTGTCAGGTCTTTGACCAGGAACTGGATGCACTGGAAATTGAGACAGTGCAA
  AAGGAGACAATCCATCCCCGAAAGTCCTATAAGATGAACTCTTCCTGTGCGGATATACTACTCTTTGCCT
  CCTATAAGTGGAATGTCTCCCGGCCCTCGCTGCTGGCAGACTCCAAGGATGTGATGGACAGCACCACCAC
  ACAGAAGTATTGGATTGACATCCAATTACGCTGGGGAGACTATGATTCCCATGATATTGAGCGCTATGCC
  CGGGCCAAGTTCCTGGATTACACCACAGACAACATGAGTATCTACCCTTCGCCCACAGGCGTGCTCATTG
  CCATTGATCTGGCCTATAATCTACACAGTGCCTATGGAAACTGGTTCCCGGGCAGCAAGCCTCTCATACA
  GCAGGCCATGGCCAAGATCATGAAGGCAAACCCTGCGTTGTATGTGTTACGTGAACGGATACGCAAAGGA
  TTACAGCTGTATTCATCTGAGCCCACTGAGCCTTACCTGTCCTCTCAGAACTATGGTGAACTCTTCTCTA
  ACCAGATTATCTGGTTTGTGGATGACACCAATGTCTATAGAGTAACTATCCACAAGACCTTTGAGGGGAA
  CTTGACAACTAAACCCATCAATGGGGCCATCTTCATCTTCAATCCACGAACGGGACAGCTGTTCCTCAAG
  ATAATCCACACATCTGTATGGGCTGGACAGAAGCGTTTGGGTCAGTTGGCCAAGTGGAAGACTGCTGAGG
  AAGTGGCTGCCCTGATCCGATCTCTGCCTGTGGAGGAACAGCCTAAACAGATCATTGTCACTCGGAAGGG
  CATGCTGGATCCTCTGGAGGTCCACTTACTGGATTTCCCCAATATTGTGATCAAAGGATCGGAGCTCCAG
  CTGCCCTTCCAGGCTTGTCTCAAGGTGGAGAAGTTTGGGGATCTCATCCTTAAAGCCACCGAGCCCCAGA
  TGGTCCTTTTCAACCTCTATGATGACTGGCTCAAGACTATCTCCTCCTACACGGCTTTCTCCCGTCTCAT
  TCTGATTCTGCGGGCTTTGCATGTGAACAATGATAGAGCAAAGGTGATCCTGAAGCCAGACAAGACTACT
  GTTACGGAACCCCACCACATCTGGCCCACGCTGACTGACGAGGAATGGATCAAGGTGGAGGTACAGCTCA
  AGGATCTGATCTTGGCTGACTACGGCAAGAAAAACAACGTGAATGTGGCGTCACTGACACAGTCAGAAAT
  TCGAGACATCATCCTGGGAATGGAGATCTCGGCGCCATCGCAGCAGCGGCAGCAGATAGCTGAGATTGAA
  AAGCAGACCAAGGAACAGTCCCAGCTCACTGCTACACAGACTCGAACTGTCAACAAGCACGGTGACGAGA
  TTATCACTTCAACCACTAGCAACTATGAGACCCAGACCTTCTCATCAAAGACTGAATGGAGAGTCAGGGC
  CATCTCTGCTGCCAACCTGCATCTGCGGACCAATCATATTTATGTTTCATCTGATGACATTAAGGAGACT
  GGTTATACGTACATTCTTCCGAAAAACGTGCTTAAGAAGTTCATCTGCATATCTGATCTTCGTGCCCAAA
  TTGCAGGATACCTGTATGGGGTGAGCCCTCCAGATAACCCTCAGGTGAAGGAGATCCGTTGTATTGTGAT
  GGTGCCGCAGTGGGGCACGCACCAGACTGTGCACCTGCCCAGCCAGCTGCCCCAGCATGAGTACCTCAAG
  GAGATGGAACCCTTAGGTTGGATCCACACTCAGCCCAATGAATCCCCCCAGCTATCACCCCAAGATGTCA
  CCACTCATGCCAAAATCATGGCTGACAACCCATCTTGGGATGGGGAGAAGACTATTATCATCACGTGCAG
  CTTCACACCAGGCTCCTGCACACTGACAGCCTACAAGCTGACACCCAGTGGGTATGAGTGGGGCCGGCAG
  AACACAGACAAAGGCAACAACCCCAAAGGCTACCTACCCTCGCACTACGAGAGGGTTCAGATGCTCCTGT
  CTGACCGATTCCTCGGCTTCTTCATGGTCCCCGCCCAGTCCTCCTGGAACTACAACTTTATGGGTGTTCG
  ACATGATCCCAACATGAAATACGAGCTGCAGCTGGCAAACCCCAAGGAATTCTACCATGAGGTCCACAGG
  CCCTCCCACTTCCTCAACTTCGCCCTTCTGCAGGAGGGTGAGGTCTACTCTGCAGACAGAGAGGACCTCT
  ATGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218624 representing NM_138659
  Red=Cloning site Green=Tags(s)
  
  MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKII
  RDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVY
  ISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDH
  QPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFE
  PLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAF
  YFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
  ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWV
  EVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDS
  HVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFW
  AAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEG
  IKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKT
  VCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
  LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYD
  VEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESR
  FEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDL
  LVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDP
  NNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMC
  GFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
  NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSM
  GHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQN
  RRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNY
  RTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSP
  TINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQ
  KETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
  RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKG
  LQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLK
  IIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQ
  LPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTT
  VTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIE
  KQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
  GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLK
  EMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQ
  NTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHR
  PSHFLNFALLQEGEVYSADREDLYA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138659
• ORF Size: 7005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138659.2, NP_619600.2
• RefSeq Size: 7500
• RefSeq ORF: 7008
• Locus ID: 192159