Uba3 (NM_001111106) Mouse Tagged ORF Clone

CAT#: MG218638

  • TrueORF®

Uba3 (GFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 3 (Uba3) transcript variant 2, (10ug)


  "NM_001111106" in other vectors (4)

Reconstitution Protocol

USD 520.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Uba3
Synonyms A830034N06Rik; AI256736; AI848246; AW546539; Ube1c
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG218638 representing NM_001111106
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATGGCGAGGAGCCAATGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCT
GGAACCATGTAAAGAAGTTCCTCGAGCGGTCTGGACCCTTCACACACCCCGATTTCGAACCAAGCACTGA
ATCACTCCAGTTCTTGTTAGATACATGTAAAGTTCTAGTCATTGGAGCTGGTGGCTTAGGATGTGAGCTT
CTGAAAAATCTGGCATTATCTGGTTTTAGACAGATTCATGTTATAGACATGGACACTATAGATGTTTCCA
ATTTAAATAGACAGTTTTTATTTAGGCCTAAAGATGTCGGAAGACCCAAGGCTGAAGTTGCTGCAGAATT
CCTAAATGACAGAGTTCCTAACTGCAACGTGGTACCACATTTCAACAAGATACAAGATTTTAACGACACT
TTCTACCGACAATTTCATATCATTGTATGTGGCCTGGACTCTATCATAGCGAGAAGATGGATCAATGGAA
TGCTGATATCTCTTCTAAATTATGAAGATGGTGTGTTGGATCCAAGCTCCATTGTACCTTTGATAGATGG
GGGGACAGAAGGCTTTAAAGGGAATGCCCGAGTGATTTTGCCTGGAATGACCGCTTGTATTGAGTGCACT
CTGGAACTTTACCCACCACAGGTCAATTTCCCCATGTGTACCATTGCATCTATGCCCAGGCTCCCAGAAC
ACTGTATCGAGTATGTGAGGATGTTGCAATGGCCTAAAGAGCAGCCTTTTGGAGATGGGGTTCCATTAGA
TGGAGATGACCCTGAACATATTCAGTGGATTTTCCAAAAGTCCATAGAGAGAGCATCACAATATAATATT
AGAGGCGTTACCTACAGACTCACTCAAGGGGTGGTAAAACGAATCATTCCTGCAGTAGCTTCTACAAATG
CAGTCATTGCAGCTGTGTGTGCCACTGAGGTTTTCAAGATAGCTACAAGTGCGTACATTCCCCTTAATAA
CTACCTGGTATTCAATGATGTAGATGGGCTGTACACTTACACGTTTGAAGCAGAGAGAAAGGAAAACTGT
CCAGCATGTAGCCAACTTCCTCAAAACATTCAGTTTTCCCCATCAGCTAAACTACAGGAGGTCTTAGACT
ACCTAACCAACAGTGCTTCTCTGCAAATGAAGTCTCCGGCTATCACAGCCACATTAGAGGGGAAGAACAG
GACACTTTACTTACAGTCAGTAACGTCTATTGAAGAACGAACCAGGCCCAATCTTTCCAAAACATTAAAA
GAACTGGGACTAGTTGATGGACAAGAACTGGCTGTTGCTGATGTCACTACACCACAGACTGTACTATTCA
AACTTCATTTTACT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG218638 representing NM_001111106
Red=Cloning site Green=Tags(s)

MADGEEPMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCEL
LKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDT
FYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECT
LELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGDGVPLDGDDPEHIQWIFQKSIERASQYNI
RGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENC
PACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLK
ELGLVDGQELAVADVTTPQTVLFKLHFT

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001111106
ORF Size 1344 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_001111106.1, NP_001104576.1
RefSeq Size 2401
RefSeq ORF 1347
Locus ID 22200
Gene Summary The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]

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