Gabrg1 (NM_010252) Mouse Tagged ORF Clone

CAT#: MG218825

Gabrg1 (GFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor subunit gamma 1 (Gabrg1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gabrg1
• Synonyms: GabaA; GabaA/BZ
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218825 representing NM_010252
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCTGGGAAAGCCTTCCTCTTCTCTCCTTCTCTCCTGTGGAGTCAAACTAGAGGAGTGAGGTTGA
  TATTCTTGTTACTAACCCTGCATCTGGGAAACTGTGTCGATAAAGCAGATGATGAAGATGATGAAGATTT
  AACTATGAACAAAACATGGGTCTTGGCACCAAAAATTCATGAAGGAGATATCACACAAATCCTCAACTCT
  TTGCTTCAAGGTTATGATAACAAACTTCGTCCAGATATAGGCGTGAGACCCACAGTGATTGAAACAGATG
  TTTATGTAAACAGCATTGGACCTGTGGATCCCATAAACATGGAGTATACAATAGACATAATTTTTGCCCA
  AACCTGGTTTGACAGCCGTTTAAAGTTCAATAGCACCATGAAAGTACTTATGCTCAACAGCAACATGGTG
  GGGAAAATTTGGATTCCCGACACTTTCTTCAGGAACTCAAGAAAATCGGATGCACACTGGATAACAACGC
  CTAATCGCCTGCTGCGGATTTGGAGTGATGGAAGGGTTCTCTATACTCTTCGATTAACAATTAATGCAGA
  GTGCTACCTTCAACTTCATAATTTTCCTATGGATGAACATTCCTGTCCACTGGAATTTTCAAGCTATGGA
  TACCCTAAAAATGAAATTGAGTACAAGTGGAAAAAGCCCTCTGTGGAAGTAGCTGATCCTAAGTACTGGA
  GATTATATCAGTTTGCATTTGTAGGGTTACGAAATTCTACTGAAATCTCTCACACAATCTCTGGAGATTA
  TATTATCATGACTATTTTTTTTGACCTGAGCAGACGGATGGGCTATTTCACGATTCAGACATACATTCCG
  TGTATTCTAACAGTGGTTCTTTCCTGGGTGTCTTTTTGGATCAATAAAGATGCAGTACCTGCTAGAACAT
  CCCTGGGTATCACTACGGTTTTGACTATGACAACCCTCAGTACAATTGCTAGAAAATCTTTACCTAAGGT
  TTCTTACGTGACAGCAATGGATCTCTTTGTCTCTGTGTGTTTCATTTTTGTGTTTGCAGCACTCATGGAA
  TACGGAACCTTGCATTATTTTACTAGCAACAATAAAGGAAAAACCACCAGAGGCAGGAAGCTGAAAAACA
  AGACTTCGGCTTCCCCAGGTCTCCATGCTGGATCCACTCTCATTCCCATGAACAGCATTTCTTTGCCACA
  AGGGGAAGATGATTATGGCTACCAGTGTTTGGAGGGCAAAGACTGTACTAGCTTTTTCTGTTGCTTCGAT
  GACTGCAGAACTGGGTCCTGGAGGGAAGGGCGGATACACATACGCATTGCAAAAATTGACTCCTACTCCA
  GAATCTTTTTCCCAACAGCTTTTGCCTTGTTCAATCTGGTTTACTGGGTTGGGTATCTATACTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218825 representing NM_010252
  Red=Cloning site Green=Tags(s)
  
  MGSGKAFLFSPSLLWSQTRGVRLIFLLLTLHLGNCVDKADDEDDEDLTMNKTWVLAPKIHEGDITQILNS
  LLQGYDNKLRPDIGVRPTVIETDVYVNSIGPVDPINMEYTIDIIFAQTWFDSRLKFNSTMKVLMLNSNMV
  GKIWIPDTFFRNSRKSDAHWITTPNRLLRIWSDGRVLYTLRLTINAECYLQLHNFPMDEHSCPLEFSSYG
  YPKNEIEYKWKKPSVEVADPKYWRLYQFAFVGLRNSTEISHTISGDYIIMTIFFDLSRRMGYFTIQTYIP
  CILTVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFAALME
  YGTLHYFTSNNKGKTTRGRKLKNKTSASPGLHAGSTLIPMNSISLPQGEDDYGYQCLEGKDCTSFFCCFD
  DCRTGSWREGRIHIRIAKIDSYSRIFFPTAFALFNLVYWVGYLYL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010252
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010252.4, NP_034382.2
• RefSeq Size: 4771
• RefSeq ORF: 1398
• Locus ID: 14405
• Gene Summary: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. [UniProtKB/Swiss-Prot Function]