Pde7b (NM_013875) Mouse Tagged ORF Clone

CAT#: MG218917

Pde7b (GFP-tagged) - Mouse phosphodiesterase 7B (Pde7b), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde7b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218917 representing NM_013875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTGTTTAATGGTTGAGAGGTGTGGCGAAGTCTTGTTTGAGAGCCCTGAACAGAGTGTCAAATGTG
  TTTGCATGCTAGGAGATGTACGACTAAGGGGTCAGACGGGGGTTCCTGCCGAACGCCGTGGCTCCTACCC
  ATTCATTGACTTCCGTCTACTTAACAATACAACACACTCAGGGGAAATTGGCACCAAGAAAAAGGTGAAA
  CGACTGTTAAGTTTCCAAAGATACTTCCATGCATCTAGGCTTCTCCGGGGGATTATACCGCAGGCCCCTC
  TCCACCTGCTGGATGAAGACTACCTTGGACAAGCAAGGCACATGCTCTCCAAAGTTGGAACGTGGGACTT
  TGACATTTTCTTGTTTGATCGCTTGACAAATGGGAACAGTCTGGTAACTCTGTTGTGTCACCTCTTCAAC
  TCCCATGGGCTCATCCACCATTTCAAGCTCGATATGGTGACCTTGCACAGGTTTCTGGTTATGGTTCAGG
  AAGATTACCACGGTCACAACCCATACCACAATGCTGTTCACGCAGCCGACGTCACCCAGGCCATGCACTG
  TTACCTGAAGGAGCCAAAGTTGGCAAGCTTCCTCACACCTCTGGACATCATGCTTGGACTACTGGCTGCA
  GCAGCTCATGACGTGGACCACCCAGGGGTCAACCAGCCATTTTTGATCAAAACTAACCACCATCTTGCCA
  ACCTGTATCAGAATATGTCTGTACTGGAGAATCACCACTGGCGATCTACAATTGGCATGCTTCGAGAATC
  ACGGCTCCTGGCTCACTTGCCAAAGGAAATGACACAGGATATCGAACAGCAGCTGGGCTCCCTCATCTTG
  GCCACGGATATCAACAGACAGAATGAGTTTCTGACCCGCTTAAAAGCTCACCTCCACAATAAAGATTTGA
  GACTGGAGAATGTACAGGACAGACACTTTATGCTTCAGATCGCCTTGAAGTGTGCTGACATTTGCAATCC
  TTGTCGTATCTGGGAGATGAGCAAGCAGTGGAGTGAAAGGGTCTGTGAGGAATTCTACAGACAAGGTGAC
  CTTGAACAGAAGTTTGAACTGGAAATCAGTCCTCTTTGTAATCAACAGAAAGATTCAATCCCTAGCATAC
  AAATTGGTTTCATGACTTACATCGTGGAGCCGCTGTTCCGGGAGTGGGCCCGGTTTACTGGGAACAGCAC
  CCTGTCGGAGAACATGCTAAGCCATCTCGCGCACAACAAAGCCCAGTGGAAGAGCCTGCTGTCCAATCAG
  CACAGACGCAGGGGCAGCGGCCAGGACCTGGCGGGCCCCGCACCTGAGACCCTGGAGCAGACAGAAGGTG
  CCACGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218917 representing NM_013875
  Red=Cloning site Green=Tags(s)
  
  MSCLMVERCGEVLFESPEQSVKCVCMLGDVRLRGQTGVPAERRGSYPFIDFRLLNNTTHSGEIGTKKKVK
  RLLSFQRYFHASRLLRGIIPQAPLHLLDEDYLGQARHMLSKVGTWDFDIFLFDRLTNGNSLVTLLCHLFN
  SHGLIHHFKLDMVTLHRFLVMVQEDYHGHNPYHNAVHAADVTQAMHCYLKEPKLASFLTPLDIMLGLLAA
  AAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWRSTIGMLRESRLLAHLPKEMTQDIEQQLGSLIL
  ATDINRQNEFLTRLKAHLHNKDLRLENVQDRHFMLQIALKCADICNPCRIWEMSKQWSERVCEEFYRQGD
  LEQKFELEISPLCNQQKDSIPSIQIGFMTYIVEPLFREWARFTGNSTLSENMLSHLAHNKAQWKSLLSNQ
  HRRRGSGQDLAGPAPETLEQTEGATP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013875
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013875.5, NP_038903.3
• RefSeq Size: 4127
• RefSeq ORF: 1341
• Locus ID: 29863
• Gene Summary: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain. [UniProtKB/Swiss-Prot Function]