Pck2 (NM_028994) Mouse Tagged ORF Clone

CAT#: MG218939

Pck2 (GFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 2 (mitochondrial) (Pck2) nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pck2
• Synonyms: 1810010O14Rik; 9130022B02Rik; PEPCK-M
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218939 representing NM_028994
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTTCCTTCCCCGCCTTCCTTGTCTCCCCGACTGCGCTCGTTCCTGTGGCCATCCCGCAGCGCCA
  GTCCAGGTGCCATGGCTGCTATGTACCTCCCCGGCCTGCGGCTTAGCCGGCACGGGCTAAGGCCCTGGTG
  CTGGTCACCGTGCCGTAGCATCCAAACCCTGCATGTGCTCAGTGGAGATATGAGTCAGCTGCCGGCTGGA
  GTTCGAGACTTTGTGGCGCGCAGTGCGCATCTGTGCCAACCAGAGGGCATCCACATCTGTGATGGGACCG
  AGGCTGAGAACACTGCCATACTGGCCCTGCTGGAAGAACAGGGTCTTATCCGCAAACTCCCCAAGTATAA
  GAACTGCTGGCTGGCCCGCACAGACCCCAAGGATGTGGCACGGGTAGAAAGCAAGACGGTGATTGTAACT
  CCTTCGCAGCGGGACACAGTGCCTCTCCTGGCTGGTGGGGCCAGGGGGCAGCTGGGCAACTGGATGTCCC
  CAGATGAGTTCCAGAGAGCTGTGGACGAGAGATTCCCAGGATGCATGCAGGGCCGCATTATGTATGTGCT
  TCCATTCAGCATGGGTCCCGTGGGCTCCCCACTCTCCCGCATTGGAGTGCAGCTCACTGACTCGGCTTAC
  GTGGTGGCCAGTATGCGTATTATGACCCGCCTGGGGACACCTGTACTTCAGGCCCTGGGAGATGGTGACT
  TCATCAAGTGTCTGCATTCAGTGGGCCAGCCCCTGACTGGACATGGGGATCCTGTGGGCCAGTGGCCGTG
  CAATCCAGAAAAAACCCTGATTGGCCACGTGCCAGACCAGCGGGAGATCGTCTCCTTCGGCAGCGGCTAT
  GGTGGTAACTCCTTGCTGGGCAAGAAGTGCTTTGCCCTGCGCATCGCCTCTCGCCTGGCCAGGGATGAGG
  GCTGGCTGGCAGAGCACATGCTGATTTTGGGCATCACCAACCCTGCAGGGAAAAAGCGCTACGTGGCAGC
  TGCTTTCCCCAGTGCCTGTGGCAAGACCAATCTGGCCATGATGAGGCCTGCATTGCCGGGCTGGAAAGTG
  GAGTGTGTGGGGGATGACATCGCCTGGATGAGGTTTGACAGTGAAGGTCAACTCCGGGCCATCAACCCTG
  AGAATGGCTTCTTTGGGGTGGCCCCTGGTACCTCTGCTGCCACCAATCCCAATGCCATGGCCACAATTCA
  GAGTAATACTCTCTTCACCAACGTGGCTGAGACCAGTGATGGCGGTGTGTACTGGGAAGGCATTGACCAG
  CCTCTTCCGCCTGGTGTCACCATAACCTCGTGGCTGGGAAAGCCCTGGAAACCTGGTGACAAGGAACCCT
  GTGCACATCCAAACTCGCGCTTTTGTGTCCCGGCTCGCCAGTGCCCCATCATGGACCCAGCCTGGGAAGC
  ACCAGAAGGTGTCCCTATTGATGCCATCATCTTTGGAGGCCGCCGACCCAAAGGGGTACCACTGGTGTAC
  GAGGCCTTCAACTGGCGTCATGGGGTGTTTGTAGGTAGCGCCATGCGCTCTGAGTCCACTGCCGCTGCGG
  AGCACAAAGGAAAGACCATTATGCATGATCCCTTTGCCATGCGGCCTTTTTTTGGCTATAACTTTGGACG
  CTACCTGGAACACTGGTTGAGCATGGAGGGACAAAAAGGTGCCCGGCTGCCTCGTATCTTCCATGTCAAT
  TGGTTCCGGAGAGATGAAGCAGGCCGCTTCCTGTGGCCAGGCTTTGGAGAGAATGCTCGTGTGCTAGACT
  GGATCTGCCGAAGATTAGAAGGAGAAGACAGTGCCCAAGAGACTCCCATTGGGCTAGTACCAAAGGAAGG
  AGCCCTGGACCTCAGTGGCCTCAGCGCAGTGGACACCAGTCAGCTGTTCTCCATCCCCAAGGACTTCTGG
  GAGCAGGAGGTTCGTGATATTCGGGGCTACCTGACAGAGCAAGTCAACCAGGACCTGCCCAAGGAGGTGT
  TGGCTGAGCTCGAGGCCCTGGAAGGACGTGTACAAAAAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218939 representing NM_028994
  Red=Cloning site Green=Tags(s)
  
  MLLPSPPSLSPRLRSFLWPSRSASPGAMAAMYLPGLRLSRHGLRPWCWSPCRSIQTLHVLSGDMSQLPAG
  VRDFVARSAHLCQPEGIHICDGTEAENTAILALLEEQGLIRKLPKYKNCWLARTDPKDVARVESKTVIVT
  PSQRDTVPLLAGGARGQLGNWMSPDEFQRAVDERFPGCMQGRIMYVLPFSMGPVGSPLSRIGVQLTDSAY
  VVASMRIMTRLGTPVLQALGDGDFIKCLHSVGQPLTGHGDPVGQWPCNPEKTLIGHVPDQREIVSFGSGY
  GGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITNPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKV
  ECVGDDIAWMRFDSEGQLRAINPENGFFGVAPGTSAATNPNAMATIQSNTLFTNVAETSDGGVYWEGIDQ
  PLPPGVTITSWLGKPWKPGDKEPCAHPNSRFCVPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVY
  EAFNWRHGVFVGSAMRSESTAAAEHKGKTIMHDPFAMRPFFGYNFGRYLEHWLSMEGQKGARLPRIFHVN
  WFRRDEAGRFLWPGFGENARVLDWICRRLEGEDSAQETPIGLVPKEGALDLSGLSAVDTSQLFSIPKDFW
  EQEVRDIRGYLTEQVNQDLPKEVLAELEALEGRVQKM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028994
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028994.2, NP_083270.1
• RefSeq Size: 3400
• RefSeq ORF: 1923
• Locus ID: 74551