Cd55b (NM_007827) Mouse Tagged ORF Clone

CAT#: MG219013

Daf2 (GFP-tagged) - Mouse decay accelerating factor 2 (Daf2), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cd55b
• Synonyms: AI323748; Daf; Daf-TM; Daf2; TM-DAF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219013 representing NM_007827
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCCGCGCAGGCGCCGGGGACTTGGTCATCACCACCACTGCTGCTGGTGCTGTCACTATCGCTGG
  TGCTGTTGTTCCAAACTGTGTGCGGAGACTGCGGCCCACCTCCAGACATTCCTAATGCCAGGCCAATCTT
  GGGCAGACACTCCAAGTTTGCTGAGCAAAGCAAAGTGGCATACTCGTGTAATAACGGCTTTAAACAAGTT
  CCAGACAAGTCAAACATAGTTGTCTGTCTTGAAAATGGCCAATGGTCGAGCCACGAAACATTCTGTGAGA
  AATCATGTGATACTCCAGAAAGACTGAGTTTTGCATCCCTCAAAAAAGAGTACTTCAACATGAATTTTTT
  CCCAGTTGGTACTATTGTGGAATATGAGTGTCGGCCAGGATTTCGAAAACAACCTTCACTCTCAGGAAAA
  TCAACTTGCCTTGAGGATTTAGTATGGTCTCCAGTTGCTCAGTTTTGTAAAAAAAAATCATGCCCTAATC
  CTAAAGATCTGGATAATGGTCACATCAACATACCAACCGGCATATTATTCGGTTCAGAAATAAACTTCTC
  ATGCAACCCAGGGTACAGGCTAGTCGGTATCACCTCTATTTTATGTACTATTATAGGAAATACTGTTGAT
  TGGGATGATGAATTTCCAGTGTGCACAGAAATATTTTGTCCAGACCCACCAAAAATCAACAATGGAATAA
  TGCGAGGGGAAAGCGATTCTTATAAATATAGCCAGGTGGTCATTTATTCATGTGACAAAGGCTTCATCCT
  GTTTGGAAATTCTACCATATATTGTACTGTGAGCAAGTCTGATGTAGGACAATGGAGCAGTCCACCACCC
  CAGTGTATAGAGGAATCTAAGGTCCCAATTAAGAAACCAGTAGTTAATGTTCCAAGTACAGGAATCCCCT
  CAACGCCTCAGAAACCCACAACAGAAAGTGTTCCAAATCCAGGAGACCAACCAACTCCTCAGAAACCTTC
  CACAGTTAAAGTTCCAGCAACCCAGCATGAACCTGATACCACGACAAGAACATCTACAGACAAAGGAGAG
  TCTAACTCAGGTGGTGACCGTTATATATATGGATTTGTTGCTGTTATTGCAATGATTGATAGCCTAATTA
  TAGTCAAAACTCTTTGGACTATTTTAAGTCCAAACAGAAGGTCTGACTTTCAAGGAAAGGAGAGGAAGGA
  TGTCTCAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219013 representing NM_007827
  Red=Cloning site Green=Tags(s)
  
  MIPAQAPGTWSSPPLLLVLSLSLVLLFQTVCGDCGPPPDIPNARPILGRHSKFAEQSKVAYSCNNGFKQV
  PDKSNIVVCLENGQWSSHETFCEKSCDTPERLSFASLKKEYFNMNFFPVGTIVEYECRPGFRKQPSLSGK
  STCLEDLVWSPVAQFCKKKSCPNPKDLDNGHINIPTGILFGSEINFSCNPGYRLVGITSILCTIIGNTVD
  WDDEFPVCTEIFCPDPPKINNGIMRGESDSYKYSQVVIYSCDKGFILFGNSTIYCTVSKSDVGQWSSPPP
  QCIEESKVPIKKPVVNVPSTGIPSTPQKPTTESVPNPGDQPTPQKPSTVKVPATQHEPDTTTRTSTDKGE
  SNSGGDRYIYGFVAVIAMIDSLIIVKTLWTILSPNRRSDFQGKERKDVSK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007827
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007827.2
• RefSeq Size: 1324
• RefSeq ORF: 1203
• Locus ID: 13137
• Gene Summary: This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. This gene is located adjacent to a closely related gene on chromosome 1 and preferentially expressed in the spleen and testis. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Dec 2015]