Mgat1 (NM_001110150) Mouse Tagged ORF Clone

CAT#: MG219048

Mgat1 (GFP-tagged) - Mouse mannoside acetylglucosaminyltransferase 1 (Mgat1) transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mgat1
• Synonyms: Mgat-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219048 representing NM_001110150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGAAGCAGACTGCAGGGCTTGTGCTTTGGGGTGCTATCATCTTTGTGGGCTGGAATGCCCTGC
  TGCTTCTCTTCTTCTGGACACGCCCAGCACCTGGCAGGCTGCCCTCAGACAGCGCCCTTGGTGATGACCC
  TGCCAGCCTCACCCGTGAGGTCATCCACCTGGCCGAGGACGCTGAGGCGGAGTTGGAGCGGCAGAGGGGA
  CTGTTGCAGCAAATCAAGGAGCATTATGCTTTGTGGAGGCAGAGGTGGAGAGTTCCCACTGTGGCCCCTC
  CAGCCTGGCCCCGTGTGCCTGTGACCCCCTCACCAGTGCAGATCCCCATCCTGGTCATTGCCTGTGACCG
  CAGCACTGTCCGGCGCTGCTTGGATAAGTTGTTGCACTATCGGCCCTCAGCTGAGCGTTTCCCCATTATT
  GTCAGTCAGGACTGTGGGCATGAAGAGACAGCACAGGTCATTGCTTCCTATGGCACTGCAGTCACACACA
  TCCGGCAGCCGGACCTGAGTAACATTGCCGTGCAGCCAGACCACCGCAAGTTCCAGGGTTACTACAAGAT
  TGCCAGGCACTACCGCTGGGCACTAGGCCAGATCTTCAACAAGTTCAAGTTCCCGGCCGCTGTGGTAGTG
  GAGGATGATCTGGAAGTGGCACCAGACTTCTTTGAGTACTTCCAGGCCACCTACCCACTGCTGAGAACAG
  ACCCCTCCCTTTGGTGTGTGTCTGCTTGGAATGACAATGGCAAGGAGCAGATGGTAGACTCAAGCAAACC
  TGAGCTGCTCTATCGAACAGACTTTTTTCCTGGCCTTGGATGGCTGCTGTTGGCTGATCTGTGGGCAGAG
  CTGGAGCCCAAGTGGCCCAAGGCCTTTTGGGACGACTGGATGCGCCGACCTGAGCAGCGGAAAGGACGGG
  CTTGTATTCGTCCAGAAATTTCAAGAACTATGACCTTTGGTCGCAAGGGTGTGAGCCACGGGCAGTTCTT
  TGACCAGCATCTTAAGTTCATCAAGCTGAACCAGCAGTTCGTCCCCTTCACCCAGTTGGACCTGTCGTAC
  CTGCAGCAGGAGGCCTATGACCGGGACTTCCTCGCCCAGGTCTATGGTGCCCCCCAGCTACAGGTGGAGA
  AAGTAAGGACCAATGATCAGAAGGAGCTGGGGGAGGTGCGGGTACAGTACACTAGCAGAGACAGCTTCAA
  GGCCTTTGCTAAGGCCCTGGGTGTCATGGATGACCTCAAGTCTGGTGTCCCCAGGGCTGGCTACCGTGGC
  ATTGTCACTTTCCAGTTTCGGGGCCGGCGTGTGCACCTGGCACCCCCACAGACATGGACTGGCTATGATC
  CTAGCTGGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219048 representing NM_001110150
  Red=Cloning site Green=Tags(s)
  
  MLKKQTAGLVLWGAIIFVGWNALLLLFFWTRPAPGRLPSDSALGDDPASLTREVIHLAEDAEAELERQRG
  LLQQIKEHYALWRQRWRVPTVAPPAWPRVPVTPSPVQIPILVIACDRSTVRRCLDKLLHYRPSAERFPII
  VSQDCGHEETAQVIASYGTAVTHIRQPDLSNIAVQPDHRKFQGYYKIARHYRWALGQIFNKFKFPAAVVV
  EDDLEVAPDFFEYFQATYPLLRTDPSLWCVSAWNDNGKEQMVDSSKPELLYRTDFFPGLGWLLLADLWAE
  LEPKWPKAFWDDWMRRPEQRKGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVPFTQLDLSY
  LQQEAYDRDFLAQVYGAPQLQVEKVRTNDQKELGEVRVQYTSRDSFKAFAKALGVMDDLKSGVPRAGYRG
  IVTFQFRGRRVHLAPPQTWTGYDPSWN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110150
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001110150.1, NP_001103620.1
• RefSeq Size: 2695
• RefSeq ORF: 1344
• Locus ID: 17308
• Gene Summary: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. [UniProtKB/Swiss-Prot Function]