Madd (NM_001177719) Mouse Tagged ORF Clone

CAT#: MG219737

Madd (GFP-tagged) - Mouse MAP-kinase activating death domain (Madd) transcript variant 10, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Madd
• Synonyms: 9630059K23Rik; IG20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219737 representing NM_001177719
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAAAAGAAGTTCTGCCCTCGGTTACTTGACTATCTCGTGATCGTAGGGGCGAGGCACCCAAGCA
  GTGACAGTGTGGCTCAGACTCCTGAGCTGCTGCGGAGGTACCCACTAGAGGATCACCCAGAGTTCCCCCT
  GCCCCCAGATGTGGTGTTCTTCTGCCAGCCAGAAGGATGTCTGAGTGTGCGGCAGCGGCGGATGAGCCTT
  CGGGACGATACCTCTTTTGTCTTCACCCTAACCGATAAGGACACCGGAGTCACCCGCTATGGCATCTGTG
  TCAACTTCTACCGTTCCTTCCAAAAGCGAATGCCAAAGGAAAAGGTGGAAGGCGGAGCAGGACCCCGTGG
  GAAGGAAGGCGCTCACACCTCTGGTGCCTCAGAAGAGGCTGCCGCTGGGAGCTCAGAGAGTGGCTCAACC
  TTGCAGCCGCCTAGTGCTGACTCCACTCCTGACGTAAACCAGTCTCCTCGGGGCAAACGTAGGGCAAAAG
  CGGGCAGCCGCTCCCGCAACAGTACCCTGACATCCCTGTGTGTGCTTAGCCACTACCCTTTCTTCTCTAC
  CTTCAGAGAGTGTCTGTATACTCTCAAACGTTTGGTAGACTGCTGTAGTGAACGGCTGCTAGGCAAGAAA
  CTGGGCATCCCTCGAGGTGTACAAAGGGACACCATGTGGCGAATCTTTACTGGATCACTGCTAGTGGAGG
  AGAAGTCCAGTGCCCTTCTGCAAGACCTTCGAGAGATTGAGGCCTGGATCTATCGGTTGCTACGTTCCCC
  AGTGCCTGTCTCTGGGCAGAAGCGAGTGGACATTGAGGTCCTGCCCCAGGAACTCCAGCAGGCCCTGACA
  TTTGCTCTTCCAGATCCATCTCGATTCACCCTAGTAGATTTCCCTCTTCACCTTCCCTTGGAACTTCTGG
  GTGTAGATGCTTGTCTTCAGGTGCTAACCTGCATCCTGTTGGAGCACAAGGTGGTGCTGCAGTCTCGAGA
  CTACAATGCACTCTCCATGTCTGTGATGGCGTTCGTGGCAATGATCTACCCCCTGGAGTACATGTTCCCG
  GTCATCCCGCTGCTTCCCACCTGCATGGCCTCGGCAGAGCAGCTACTCTTGGCTCCAACCCCATACATCA
  TTGGAGTTCCTGCCAGCTTCTTCCTCTACAAGCTAGATTTCAAAATGCCCGATGACGTGTGGCTAGTGGA
  TCTGGACAGCAATAGGGTGATTGCCCCCACCAATGCAGAAGTGCTACCCATCCTGCCAGAACCAGAATCA
  TTAGAGCTGAAAAAACACTTAAAGCAGGCCTTAGCCAGCATGAGTCTCAACACCCAGCCCATTCTTAACC
  TGGAGAAATTCCACGAGGGCCAGGAGATCCCGCTTCTCTTGGGAAGGCCATCTAATGACCTTCAGTCCAC
  ACCTTCCACTGAATTCAACCCGCTCATTTATGGCAATGATGTAGATTCAGTCGACGTTGCAACGAGAGTG
  GCCATGGTACGTTTCTTCAACTCTGCTAACGTGCTGCAGGGCTTTCAGATGCACACACGTACCCTGCGAC
  TCTTCCCCCGGCCTGTGGTAGCTTTCCAAGCTGGCTCCTTTCTGGCCTCACGTCCCCGGCAGACTCCCTT
  TGCTGAGAAACTGGCCAGGACCCAAGCTGTGGAGTATTTTGGAGAATGGATCCTGAACCCTTCTAACTAT
  GCCTTCCAGCGGATTCACAACAATACGTTTGATCCGGCCCTTATTGGTGACAAGCCAAAGTGGTACGCTC
  ACCAGCTGCAGCCCATCCATTATCGAGTCTATGATGGCAACTCTCAGCTGGCCGAGGCCCTGAGTGTGCC
  CCCGGAGCGCGATTCTGACTCCGACCCGACGGAGGACAGTGGCAGTGATAGTCAGGATTATGATGACTCA
  AGCTCTTCTTACTCCTCCCTTGGGGACTTTGTCAGTGAAATGATGAAATGTGACATCAATGGAGACACTC
  CCAATGTGGATCCTCTGACCCATGCAGCCCTGGGAGATGCCAGTGAAGTGGAGATTGACGAGCTGCAGCC
  CCAGAAGGAGGGAGAGGAACCCGGCCCAGACAGTGAGAACTCTCAGGAAAACCCTCCGCTGCGCTCCAGC
  TCCAGCACCACGGCCAGCAGCAGCCCCAGCACCGTTGTCCACAGTGCCCACTCTGAAGCTGCTGACTCTA
  CAGAGATGGGAGATAAGGCCACAGCAGGCATCTCCAAGCCCCTCCCTCCCGTGCCTCCCAGCATTTGCAA
  ATCTACTGTGGACAGGAGACAGACAGAGACTGGAGAGGGGTCAGTGTGCCAGCGAACCTATGACAATCCA
  TACTTCGAGCCCCAGTATGGCTTTCCCCCCGAGGAAGATGAGGAGGAGCAGGGGGAGAGCTACACTCCCC
  GGTTCAGCCAGCATGTCAGTGGCAGTCGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAAGCTGGCAAG
  TGACTCAGACGCAGAGTCAGACTCGAGAGCAAGCTCTCCCAACTCCACTGTCTCTAACAACAGCACCGAG
  GGCTTCGGGGGCATCATGTCTTTTGCTAGCAGCCTGTATCGGAACCATAGTACAAGCTTCAGTCTTTCAA
  ACCTCACACTGCCCACCAAAGGTGCCCGCGAGAAGACGACACCATTCCCCAGCCTGAAAGTATTTGGGCT
  AAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCTGGGAGTGGTGAAGGAAATAGGAGGGCCTTAGTG
  GACCAGAAGTCATCTGTCATTAAACACAGCCCAACCGTGAAAAGAGAACCTTCATCCCCACAGGGCCGGT
  CGAGCAACTCTAGCGAGAACCAGCAGTTCCTGAAGGAAGTAGTCCATAGTGTGCTGGACGGCCAGGGAGT
  GGGCTGGCTCAATATGAAGAAGGTACGCCGGCTCCTGGAGAGTGAGCAGCTTAGAGTCTTTGTACTGAGC
  AAGCTGAATCGCGCAGTGCAGTCAGAGGATGACGCCCGGCAGGATGTCATCCAAGACGTGGAGATCAGCC
  GGAAGGTGTACAAGGGGATGCTAGACCTCCTCAAGTGCACAGTCCTCAGCCTCGAGCAGTCCTATGCCCA
  CGCAGGTCTGGGTGGTATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCACTACTATAGTAAA
  GAACCAGACAAACGGAAGAGAAGTCCGACAGAGAATGTAAATACCCCAGTTGGCAAGGATCCTGGTCTGG
  CTGGGCGGGGGGACCCAAAGGCAATGGCCCAGCTAAGGGTCCCTCAACTGGGCCCTCGGGCACCAAGTGC
  TACAGGAAAGGGTCCTAAAGAACTGGATACCAGAAGTTTAAAGGAAGAGAATTTTGTAGCATCTGTTGAA
  TTGTGGAACAAGCACCAGGAAGTGAAAAAGCAAAAGGCTTTGGAAAAACAGAGGCCAGAGGGCATCAAGC
  CTGTCTTTGACCTGGGTGAGACAGAGGAGAAAAAGTCCCAGATGAGCGCAGACAGTGGTGTGAGCCTGAC
  ATCTGCCTCCCAGAGGACTGATCAAGACTCTGTCATCGGTGTGAGTCCAGCTGTTATGATCCGAAGCTCA
  AGTCAGGATTCTGAAGTTAGCACCGTGAGTAATAGTTCCGGAGAGACCCTTGGAGCTGACAGTGACCTGA
  GCAGCAATGCAGGTGACGGGCCAGGAGGCGAAGGCAGCGCCCACCTGGCAAGTTCCCGGGCCACTCTGTC
  CGACAGTGAAATCGAAACCAATTCTGCTACAAGCGCCATCTTTGGTAAAGCCCATAGCTTGAAGCCAAAG
  GAGAAGCCGGCTGGTAGTCCGATTCGCTCTTCCGAAGACGTAAGCCAGCGAGTCTATCTCTATGAGGGAC
  TGCTAGGAAGGGACAAAGGATCCATGTGGGACCAGCTAGAGGATGCCGCTATGGAGACCTTTTCTCTGAG
  CAAAGAACGTTCTACCTTATGGGACCAAATGCAGTTCTGGGAAGATGCATTCTTAGATGCTGTAATGTTG
  GAAAGAGAAGGAATGGGTATGGACCAGGGTCCTCAGGAAATGATCGACAGGTACCTCTCCCTAGGGGAAC
  ACGACCGGAAGCGCCTGGAGGATGATGAAGACCGCTTGCTGGCCACACTTTTACACAACCTCATATCCTA
  TATGCTGCTGATGAAGGTGAACAAGAATGACATCAGGAAGAAAGTACGGCGCCTAATGGGAAAGTCCCAC
  ATTGGGCTGGTGTACAGCCAGCAAGTCAATGAGGTGCTCGATCAGCTGAACAGCCTGAATGGACGTGATC
  TCTCCATCCGGTCCAGCGGCAGCCGGCACATGAAGAAGCAGACTTTTGTTGTGCATGCAGGGACGGACAC
  AAATGGAGACATCTTTTTCATGGAAGTGTGTGATGACTGCGTGGTGTTGCGTAGTAACATTGGGACGGTG
  TACGAGCGCTGGTGGTATGAGAAGCTCATCAATATGACCTACTGTCCCAAGACCAAGGTCCTGTGCTTAT
  GGCGGAGAAATGGCTCTGAGACCCAGCTCAACAAGTTCTATACCAAGAAGTGTCGAGAGCTGTACTACTG
  TGTGAAGGACAGCATGGAACGTGCTGCTGCCCGACAGCAAAGCATCAAACCTGGGCCTGAGCTAGGCGGT
  GAGTTCCCTGTGCAGGACATGAAGACTGGAGAGGGTGGCTTGCTTCAAGTCACCCTGGAAGGGATCAATC
  TCAAGTTCATGCACAACCAGTTCCTGAAATTAAAGAAGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219737 representing NM_001177719
  Red=Cloning site Green=Tags(s)
  
  MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSL
  RDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGST
  LQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKK
  LGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALT
  FALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFP
  VIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPES
  LELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRV
  AMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNY
  AFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDS
  SSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSS
  SSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNP
  YFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTE
  GFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALV
  DQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLS
  KLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSK
  EPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVE
  LWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSS
  SQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPK
  EKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVML
  EREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSH
  IGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTV
  YERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGG
  EFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177719
• ORF Size: 4731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001177719.1, NP_001171190.1
• RefSeq Size: 6151
• RefSeq ORF: 4734
• Locus ID: 228355