Hpn (NM_001110252) Mouse Tagged ORF Clone

CAT#: MG219750

Hpn (GFP-tagged) - Mouse hepsin (Hpn) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hpn
• Synonyms: Hlb320
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219750 representing NM_001110252
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGGAGGATGAGGAACCTGGGGCTCACAGAGGAGGTTCCACTTGTTCAAGACCCCAACCTGGAA
  AGGGTGGCCGGACTGCAGCATGCTGCTCCAGACCCAAGGTGGCAGCTCTCATTGTGGGTACCCTGCTGTT
  CCTGACAGGCATTGGGGCCGCGTCCTGGGCCATTGTGACCATCCTACTGCAGAGTGACCAGGAGCCACTG
  TACCAAGTGCAGCTCAGTCCAGGGGACTCACGGCTTGCGGTGTTTGACAAGACGGAGGGAACGTGGAGGC
  TACTGTGCTCCTCACGCTCCAATGCCAGGGTGGCAGGGCTCGGCTGTGAGGAGATGGGCTTTCTCAGGGC
  TCTGGCGCACTCGGAGCTGGATGTGCGCACTGCGGGCGCCAACGGCACATCGGGCTTCTTTTGCGTGGAC
  GAGGGCGGACTGCCTCTGGCTCAGAGGTTGCTGGATGTCATCTCTGTATGTGACTGTCCTAGAGGCCGAT
  TCCTGACTGCCACCTGCCAAGACTGTGGCCGCAGGAAGCTGCCGGTGGACCGCATTGTGGGGGGCCAGGA
  CAGCAGTCTGGGAAGGTGGCCGTGGCAGGTCAGCCTGCGTTATGATGGGACCCACCTCTGTGGGGGGTCC
  CTGCTGTCTGGGGACTGGGTGCTGACTGCTGCACATTGCTTTCCAGAGCGGAACCGGGTCCTGTCTCGGT
  GGCGAGTATTTGCTGGTGCTGTAGCCCGGACCTCACCCCATGCTGTGCAACTGGGGGTTCAGGCTGTGAT
  CTATCATGGGGGCTACCTTCCCTTTCGAGACCCTACTATCGACGAAAACAGCAATGACATTGCCTTGGTC
  CACCTCTCTAGCTCCCTGCCTCTCACAGAATACATCCAGCCAGTGTGTCTCCCTGCTGCGGGACAGGCCC
  TGGTGGATGGCAAGGTCTGTACTGTGACCGGCTGGGGTAACACACAGTTCTATGGCCAACAGGCTATGGT
  GCTCCAAGAGGCCCGGGTTCCCATCATAAGCAACGAAGTTTGCAACAGCCCCGACTTCTACGGGAATCAG
  ATCAAGCCCAAGATGTTCTGTGCTGGCTATCCTGAGGGTGGCATTGATGCGTGCCAGGGCGACAGTGGAG
  GCCCCTTTGTGTGTGAAGACAGCATCTCTGGGACATCAAGGTGGCGGCTATGTGGCATTGTAAGCTGGGG
  TACGGGCTGTGCTTTGGCCCGGAAGCCAGGAGTGTACACCAAAGTCACTGACTTCCGGGAGTGGATCTTC
  AAGGCCATAAAGACTCACTCCGAAGCCAGTGGCATGGTGACTCAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219750 representing NM_001110252
  Red=Cloning site Green=Tags(s)
  
  MAKEDEEPGAHRGGSTCSRPQPGKGGRTAACCSRPKVAALIVGTLLFLTGIGAASWAIVTILLQSDQEPL
  YQVQLSPGDSRLAVFDKTEGTWRLLCSSRSNARVAGLGCEEMGFLRALAHSELDVRTAGANGTSGFFCVD
  EGGLPLAQRLLDVISVCDCPRGRFLTATCQDCGRRKLPVDRIVGGQDSSLGRWPWQVSLRYDGTHLCGGS
  LLSGDWVLTAAHCFPERNRVLSRWRVFAGAVARTSPHAVQLGVQAVIYHGGYLPFRDPTIDENSNDIALV
  HLSSSLPLTEYIQPVCLPAAGQALVDGKVCTVTGWGNTQFYGQQAMVLQEARVPIISNEVCNSPDFYGNQ
  IKPKMFCAGYPEGGIDACQGDSGGPFVCEDSISGTSRWRLCGIVSWGTGCALARKPGVYTKVTDFREWIF
  KAIKTHSEASGMVTQP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110252
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001110252.1, NP_001103722.1
• RefSeq Size: 1830
• RefSeq ORF: 1311
• Locus ID: 15451
• Gene Summary: This gene encodes a type II transmembrane serine protease that may function in diverse processes, including regulation of cell growth. Deficiency in this gene results in hearing loss. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]