Smox (NM_001177833) Mouse Tagged ORF Clone

CAT#: MG219787

Smox (GFP-tagged) - Mouse spermine oxidase (Smox) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smox
• Synonyms: B130066H01Rik; PAO; PAOh1; SMO
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219787 representing NM_001177833
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAGTTGTGAATCCAGTGGCGACAGTGCGGATGACCCTCTCAGTCGTGGCCTACGGAGAAGGGGAC
  AGCCTCGTGTGGTGGTGATCGGTGCTGGCTTGGCTGGCCTGGCTGCAGCTAGAGCCCTTCTGGAGCAGGG
  CTTCACGGATGTCACTGTGCTTGAGGCTTCCAGCCACATTGGGGGCCGTGTGCAGAGTGTGAGGCTTGGA
  GACACCACCTTTGAGCTGGGAGCCACCTGGATCCATGGATCCCACGGGAATCCTATCTATCAACTAGCAG
  AAGCCAATGGCCTTTTGGAAGAGACAACAGATGGGGAGCGCAGTGTGGGCCGCATCAGCCTTTACTCCAA
  GAATGGCGTGGCCTGCTACCTTACCAACCGTGGCTGCCGCATCCCCAAGGACGTGGTTGAGGAATTCAGC
  GATTTATACAACGAGGTCTATAACATGACCCAGGAGTTCTTCCGGCATGGTAAACCAGTCAATGCCGAGA
  GTCAGAACAGCGTCGGGGTGTTCACCCGGGAGAAGGTGCGGAATCGCATCAGGGATGACCCTGACGACAC
  AGAGGCCACCAAGCGCCTGAAGCTCGCCATGATCCAGCAGTACCTGAAGGTGGAGAGCTGTGAGAGCAGC
  TCCCACAGCATAGATGAGGTGTCCCTGAGCGCCTTTGGAGAATGGACGGAGATCCCAGGCGCCCATCACA
  TCATCCCCTCGGGCTTCATGCGAGTTGTGGAGCTGCTGGCTGAGGGCATTCCTCCACATGTCATCCAGTT
  GGGGAAGCCGGTCCGTTGCATCCACTGGGACCAGGCCTCGGCTCACCCCCGGGGTCCTGAGATCGAGCCC
  CGTGGTGAGGGTGATCACAATCACGACACTGGGGAGGGTGGCCAGAGTGGAGAGAATCCGCAGCAGGGGA
  GGTGGGACGAGGATGAGCCGTGGCCTGTAGTCGTGGAGTGCGAGGATTGCGAGGTGATCCCAGCGGACCA
  CGTGATTGTGACCGTTTCGCTGGGCGTGCTCAAGAGGCAGTACACCAGTTTCTTTAGGCCATGCCTGCCC
  ACGGAGAAGGTGGCCGCCATCCACCGCCTGGGCATTGGTACCACTGACAAGATCTTCCTTGAATTTGAGG
  AGCCCTTTTGGGGCCCCGAGTGCAACAGCCTGCAGTTCGTGTGGGAGGATGAGGCAGAGAGCTGTACCCT
  CACCTACCCACCTGAGCTCTGGTACCGCAAGATCTGTGGCTTCGATGTCCTTTATCCGCCAGAGCGCTAT
  GGCCATGTGCTGAGTGGCTGGATCTGTGGGGAGGAGGCTCTTGTCATGGAGAGGTGCGATGACGAGGCTG
  TAGCTGAGATCTGCACAGAGATGCTTCGACAGTTCACAGGGAACCCCAATATACCAAAACCTAGGCGAAT
  CCTGCGCTCAGCCTGGGGCAGCAACCCATACTTCCGGGGTTCCTATTCCTACACACAGGTGGGCTCAAGT
  GGGGCGGATGTGGAGAAGCTAGCCAAGCCCCTGCCCTACACAGAGAGCTCCAAGACAGCGCATAGAAGCT
  CCACAGAGCAGCAGCCTGGTCACCTTTTACCATCCAAGTGCCCAGAACAATCCCTGGACCCTAGTAGGGG
  CTCCATAAAGCCCATGCAGGTGCTCTTCTCCGGGGAGGCCACACACCGCAAGTACTACTCCACCACCCAC
  GGTGCTCTGCTCTCTGGCCAGCGCGAGGCCGCCCGGCTCATCGAGATGTACCGAGACCTCTTCCAGCAGG
  GGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219787 representing NM_001177833
  Red=Cloning site Green=Tags(s)
  
  MQSCESSGDSADDPLSRGLRRRGQPRVVVIGAGLAGLAAARALLEQGFTDVTVLEASSHIGGRVQSVRLG
  DTTFELGATWIHGSHGNPIYQLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNRGCRIPKDVVEEFS
  DLYNEVYNMTQEFFRHGKPVNAESQNSVGVFTREKVRNRIRDDPDDTEATKRLKLAMIQQYLKVESCESS
  SHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPPHVIQLGKPVRCIHWDQASAHPRGPEIEP
  RGEGDHNHDTGEGGQSGENPQQGRWDEDEPWPVVVECEDCEVIPADHVIVTVSLGVLKRQYTSFFRPCLP
  TEKVAAIHRLGIGTTDKIFLEFEEPFWGPECNSLQFVWEDEAESCTLTYPPELWYRKICGFDVLYPPERY
  GHVLSGWICGEEALVMERCDDEAVAEICTEMLRQFTGNPNIPKPRRILRSAWGSNPYFRGSYSYTQVGSS
  GADVEKLAKPLPYTESSKTAHRSSTEQQPGHLLPSKCPEQSLDPSRGSIKPMQVLFSGEATHRKYYSTTH
  GALLSGQREAARLIEMYRDLFQQGP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177833
• ORF Size: 1755 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001177833.1, NP_001171304.1
• RefSeq Size: 2280
• RefSeq ORF: 1758
• Locus ID: 228608
• Gene Summary: Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal. [UniProtKB/Swiss-Prot Function]