Grxcr2 (NM_001033426) Mouse Tagged ORF Clone

CAT#: MG219836

Grxcr2 (GFP-tagged) - Mouse glutaredoxin cysteine rich 2 (Grxcr2), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grxcr2
• Synonyms: Gm851
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219836 representing NM_001033426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACTCCGAGAAGAAACTGAATCAGAAGAGCGACGACAAACCCAGAAAAGTCCGGTTTAAAATCT
  CCTCTTCCTACAGTGGCCGAGTGTTGAAGCAGGTTTTTGAGGATGGGCAAGAATTAGAGTCGCCCAAAGA
  AGAATATCCTCACAGTTTTCTCCAGGAGGCCCTTGAACCAATGGATGGCGTTTATGGGTCTGGGGAAGTC
  CCCAAGCCCCAGCCGTACTCCCCCAAGCTGACTGCTCAGAGAATCAGCGTATTCAGAGACAGCGGCGCCT
  ACACTCTGGCGGGCAGCCAGCCTCTGTTCAATGACTACAAGGCGAATGACCATAAGCCTCCACCCATTAT
  AGACTTTGGGAAGATAATTATCTATACCAACAACCTGAAAATCATCAGAACCCCGATGGACAAAAGGGAC
  TTCATGAGGAAAATCCTCCAAAAGGAAGATGTGGCTGAGGAAGCGTCCCTGATGATCACAGGGGAGAATG
  ATGGAGACAGGGAGCAAGGCTGCCCCCTGCCGGAGCGGAACGGCAGCCCCCTGCCGGAGTCGGAGCGCAC
  ATTTCTTCATAGTCAGCACACACAGGATGGGTTGGTCCCTGAGGACTGCCTCCACTGCCAGGGGTCTGGC
  ATTGCCACCTGTTCTCTGTGCCACGGCAGCAAGTTCTCCATGCTGGCTAACAGATTCAAGGAGTCCTACC
  GAGCCCTCAGGTGCCCTGCCTGCAACGAGAATGGCCTGCAACCTTGCCGAATTTGCAGCCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219836 representing NM_001033426
  Red=Cloning site Green=Tags(s)
  
  MEDSEKKLNQKSDDKPRKVRFKISSSYSGRVLKQVFEDGQELESPKEEYPHSFLQEALEPMDGVYGSGEV
  PKPQPYSPKLTAQRISVFRDSGAYTLAGSQPLFNDYKANDHKPPPIIDFGKIIIYTNNLKIIRTPMDKRD
  FMRKILQKEDVAEEASLMITGENDGDREQGCPLPERNGSPLPESERTFLHSQHTQDGLVPEDCLHCQGSG
  IATCSLCHGSKFSMLANRFKESYRALRCPACNENGLQPCRICSP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033426
• ORF Size: 762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033426.2, NP_001028598.1
• RefSeq Size: 1120 bp
• RefSeq ORF: 765 bp
• Locus ID: 332309
• Cytogenetics: 18 B3