Grk4 (NM_019497) Mouse Tagged ORF Clone

CAT#: MG219941

Grk4 (GFP-tagged) - Mouse G protein-coupled receptor kinase 4 (Grk4) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grk4
• Synonyms: A830025H08Rik; Gprk2l; Gprk4; GRK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219941 representing NM_019497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTCGAGAACTTCGTGGCCAACAATCTGTTGCTGAAGGCCCGTCTAGGATTTAACAAACAGACTG
  GTCGTAGTAAAAAATGGAGAGAGCTCCTGAAGTTTCCCCCAGTCAGCATGTGCACTGAATTAAGATGGTC
  CATCGAAAAGGATTTTAGCAGTCTTTGCGACAAACAGCCAATTGGAAGACTTCTTTTTAGACAATTCTGT
  GATACAAAACCAGATCTAAAAAGGTGCATTGAATTCTTGGATGCAGTGGCAGAGTACGAAGTTACCATTG
  AAGAGGAACAAAGAGAATTTGGACTAGCCATCTTCAGTAGATTCTTCAAAGAGAAGTCAGAAGTCCCCTT
  ACCAGAGATACCTCCAGATATTGTGAAAGAGTGTAAATGGAATCTGAAGCAGAATTCTCCCTCTCAAAAT
  GTTTTCGAAGAATGTGCTGGAATTGTCTGTAAGTATTTGAGTGAAACACCATTTGAAGAATACCAAGAAA
  GTACCTACTTTAATCGATTTTTACAATGGAAATGGCTAGAAAGGCGTCCAGTAACAAAGAACACATTCAG
  ACAATATAGAGTTCTAGGAAAAGGCGGATTTGGAGAGGTTTGTGCCTGTCAAGTACGAGCTACAGGAAAA
  ATGTATGCCTGCAAAAAACTAGAAAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCCATGGCTCTGAATG
  AAAAAAGAATCTTAGAAAAATTGCATAGTAGATTTGTAGTTAGTTTAGCCTACACTTATGAAACCAAGGA
  TGCTTTGTGTTTGGTGCTAACCATAATGAATGGAGGGGATTTGAAGTACCACATTTACAACCTGGGTGAT
  CCTGGCTTTGAGGAGCCGAGAGCTGTGTTCTATGCTGCAGAGCTGTGCTGTGGCTTGGAAGATTTACAGA
  GGAAAAGAATTGTATACAGAGACCTAAAGCCAGAGAATATCCTCCTTGATGATCATGGACACATCCGAAT
  TTCAGATCTTGGTCTAGCTATGGAAGTCCCAGAAGGGGAGATGGTACGAGGAAGAGTTGGTACTGTTGGC
  TACATGGCTCCAGAAATTATCAATCATGAAAAGTATACATTTAGTCCTGACTGGTGGGGACTTGGCTGTT
  TGATATATGAAATGATTGCGGGACATTCTCCATTCAGAAAATATAAAGAAAAAGTCAACCGGGAGGAATT
  GGAAAGAAGAGTGAAGAATGAAACTGAGGAGTACTCTGAGAGGTTTTCAGAGGATGCCAAGTCCATCTGC
  AGCATGTTACTCATCAAAGATCCAAGTAAGAGACTGGGCTGCCAGAGGGATGGAGTCTCTGCTGTAAAGC
  AGCACCCAATATTCAAGGATATAAACTTCAGCAGGCTGGAAGCAAATATGTTAGATCCACCTTTCATTCC
  TGATCCTCAGGCCATTTATTGTAGGAACATCTTGGATATTGGGCAGTTCTCTGTGGTGAAAGGAGTCAAC
  CTGGATACCAATGATGAAATCTTCTACGCTGAATTTGCGACAGGGTCTGTCACCATCCCCTGGCAGAATG
  AGATGATTGAATCTGGATGTTTCAAGGATCTCAATGAAAATGAAGACGATTTGTCATCACTTGAAAAATA
  CAAGATGTGTTCCTCCATTCTCAGACCAAAGAGAAACTTCTTCCGTAGACTCTTCAGAAGAACGGGCTGC
  CTGAACATTGCCCTCAGTGAGGAGAGGGAGCCAACAGAACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219941 representing NM_019497
  Red=Cloning site Green=Tags(s)
  
  MELENFVANNLLLKARLGFNKQTGRSKKWRELLKFPPVSMCTELRWSIEKDFSSLCDKQPIGRLLFRQFC
  DTKPDLKRCIEFLDAVAEYEVTIEEEQREFGLAIFSRFFKEKSEVPLPEIPPDIVKECKWNLKQNSPSQN
  VFEECAGIVCKYLSETPFEEYQESTYFNRFLQWKWLERRPVTKNTFRQYRVLGKGGFGEVCACQVRATGK
  MYACKKLEKKRIKKRKGEAMALNEKRILEKLHSRFVVSLAYTYETKDALCLVLTIMNGGDLKYHIYNLGD
  PGFEEPRAVFYAAELCCGLEDLQRKRIVYRDLKPENILLDDHGHIRISDLGLAMEVPEGEMVRGRVGTVG
  YMAPEIINHEKYTFSPDWWGLGCLIYEMIAGHSPFRKYKEKVNREELERRVKNETEEYSERFSEDAKSIC
  SMLLIKDPSKRLGCQRDGVSAVKQHPIFKDINFSRLEANMLDPPFIPDPQAIYCRNILDIGQFSVVKGVN
  LDTNDEIFYAEFATGSVTIPWQNEMIESGCFKDLNENEDDLSSLEKYKMCSSILRPKRNFFRRLFRRTGC
  LNIALSEEREPTEH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019497
• ORF Size: 1722 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019497.2, NP_062370.2
• RefSeq Size: 2408
• RefSeq ORF: 1725
• Locus ID: 14772
• Gene Summary: Specifically phosphorylates the activated forms of G protein-coupled receptors. [UniProtKB/Swiss-Prot Function]