Ankef1 (NM_175667) Mouse Tagged ORF Clone

CAT#: MG219992

Ankef1 (GFP-tagged) - Mouse ankyrin repeat domain 5 (Ankrd5), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ankef1
• Synonyms: Ankrd5; AV276460
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219992 representing NM_175667
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGGCAGACAAGAGACTTGAGAACTTACAGATCTACAGAGTTCTTCAGTGTGTTCGCAACAAAG
  ACAAGAAGCAGATAGAAAAGCTGACCAGGCTTGGGTACCCTGAGCTAATCAACTTCACCGAACCCATAGA
  TGGGCTGAGTGCTCTCCACTTAGCCTCCATTTCCAACGACACTGACATGGTCAGCTTCCTCCTCAAACTT
  GGTGCGCATCCTGATGTGCAAGACCACATGGGCTGTACCCCGACCATGAGAGCTGCCGAGCTGGGCCATG
  AGTTGTCAATGGAAATTTTAGCCAAGGCCAAAGCTGATATGACAATAGTTGATAATGAAGGAAAAGGTGT
  TCTGTTTTACTGCATCCTGCCCACTAAGCGGCACTATCGATGCTCACTGATTGCCTTGGAGCATGGTGCA
  GATGTCAACAATATCACCTATGAAGGGAAGCCAGTGTTCCTCAGAGCTTGCGAGGAAGCACATGATGTGA
  AGGATATGTGCCTGACATTTTTGGAGAAAGGAGCCAATCCTAATGCTATCAACACATCCACAGGACGCAC
  GGCTTTGATGGAATCGTCAAGAGAAGGGGTGCTGGAAATAGTTCGTGGCATATTGGAAAGAGGAGGTGAA
  GTGAACGCATATGACAATGATAGACACCACGCTGCTCATTTTGCTGCCAAAGGAGGCTTTTTTGATATTC
  TGAAGCTCCTCTTTGCCTACAATGGAGACATGGGGTTAATTGGAATGGATGGGAACACACCACTTCACTT
  TGCTGCCATGGGGGGTTTTGCAGATTGCTGTAAATATATAGCTCAGCGAGGATGTGACCTGAAATGGAAA
  AATTTGGAACATAAAACACCCCGAGTTGTGGCGAAAGACGGAGGCTTTAAAGCTGCTAGCAAGGAAATAC
  GTCGAGCAGAGCGGACTGCCGCTAAACTCGCTAAGACAGGAGCAAAAAATCCTAACCCTCTCTGGGCCCT
  CAGGCTGCATGACTGGTCCATAGAGCACGAGACTTCCCTTCGGAACGCCTTTAAGTTCGTGGACAGGGGC
  GATGGCGTAGTCAGCAAAGATGACTTCGTGGTGGCCCTGGAGGAGAGGCAAGAGTATGCCACCTCAGAGC
  AGTTGCTTTCCGTTGCTCAAATGCACGAAAAAAGTCGAGGAGGCGGGGTCAACATTAATGAGTTCTTTAA
  GGGAACCAAATATTTAAGCAAGTCGTACGTATTGGGATCTTTCGGGCCTAAGAAAAAGAAAAGGGGGTTG
  GGCAAAAAGCCAAGGAAAGGCAAGTTTGTTTTGCCTCTCCCCATCTGCACCATCCCGGAGAACGCCTTCC
  CGCGGCGGCCAGATGGGGGCCCGCCCTACTACATGATTGAGACCTACCAGAATGTCTCCGACAGCCACAG
  GTTCAACCGGGACCACCCGCCCGAGCACCCAATCCAGGATGATTCCGAGTGGTACATCGATGATCCGAGT
  AGGGTCTTCGCAAATATTAGTTTTATCACCAAAGCAGGAGATCTGGCCTCTCTGAAAAAGGCCATCGAAA
  CAGGAATACCCGTGGATATGAAGGACAATACTTACAAGACTCCTCTCATGATAGCGTGTGCCAGTGGCAA
  CATTGATGTGGTCAAGTTTCTTATCGAAAAGGGGGCAAATGTTAATGCAACAGATAATTTTCTATGGACT
  CCACTTCATTTTGCATGCCATGCTGGCCAGCAAGACATTGTTGAGCTTCTTGTTAAAGCTGGAGCTTCAA
  TAGATGCAACATCAATCAACAACTCCACTCCGTTAAGCAGAGCCATTGAAAGCTGTAGGCTGGACACTGT
  AAAATACCTACTTGATATGGGTGCCAAATTCCAGATTGAAAACAGAAAAGGACATGCTGCCATGGATATT
  GCAAAGGCATATGCTGATTATAGAATAATTGATATGATAAAAGAAAAGCTAGATAACCTGCCTAAACAAG
  CAGATAATCAGAAAATGAAAGGCAAGCTTCCTAAACTGAAGACAGAAGGCACGGATGTTAAGAAAGAAGA
  GGAAACACTGTCATCCATTTACACTGTGCCAGCAATAACAGAGGAGAAGAAAGTCCACAGGGATAGTGTG
  GTTTACCTCAATTCCCTGATTACCAGTGGCTTCACCAAGAAAGTTGATATCACATTTATCCCCAAAAGGA
  TTTGGAGTCCTGAAGCCACAACAGCAGAGCTGATCAGGAAGAGGGAGCTCCGGAGGGAGAGGTTCACATA
  CGAGGTGGACTTTGAAGACTTCATGATGCCTTTCCAAAAGAACATCACGGAGAAAGCTCAGGCACTGGAG
  GCAACACTCAAGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219992 representing NM_175667
  Red=Cloning site Green=Tags(s)
  
  MALADKRLENLQIYRVLQCVRNKDKKQIEKLTRLGYPELINFTEPIDGLSALHLASISNDTDMVSFLLKL
  GAHPDVQDHMGCTPTMRAAELGHELSMEILAKAKADMTIVDNEGKGVLFYCILPTKRHYRCSLIALEHGA
  DVNNITYEGKPVFLRACEEAHDVKDMCLTFLEKGANPNAINTSTGRTALMESSREGVLEIVRGILERGGE
  VNAYDNDRHHAAHFAAKGGFFDILKLLFAYNGDMGLIGMDGNTPLHFAAMGGFADCCKYIAQRGCDLKWK
  NLEHKTPRVVAKDGGFKAASKEIRRAERTAAKLAKTGAKNPNPLWALRLHDWSIEHETSLRNAFKFVDRG
  DGVVSKDDFVVALEERQEYATSEQLLSVAQMHEKSRGGGVNINEFFKGTKYLSKSYVLGSFGPKKKKRGL
  GKKPRKGKFVLPLPICTIPENAFPRRPDGGPPYYMIETYQNVSDSHRFNRDHPPEHPIQDDSEWYIDDPS
  RVFANISFITKAGDLASLKKAIETGIPVDMKDNTYKTPLMIACASGNIDVVKFLIEKGANVNATDNFLWT
  PLHFACHAGQQDIVELLVKAGASIDATSINNSTPLSRAIESCRLDTVKYLLDMGAKFQIENRKGHAAMDI
  AKAYADYRIIDMIKEKLDNLPKQADNQKMKGKLPKLKTEGTDVKKEEETLSSIYTVPAITEEKKVHRDSV
  VYLNSLITSGFTKKVDITFIPKRIWSPEATTAELIRKRELRRERFTYEVDFEDFMMPFQKNITEKAQALE
  ATLKN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175667
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175667.4, NP_783598.1
• RefSeq Size: 2723
• RefSeq ORF: 2328
• Locus ID: 319196