Mefv (NM_001161790) Mouse Tagged ORF Clone

CAT#: MG220043

Mefv (GFP-tagged) - Mouse Mediterranean fever (Mefv) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mefv
• Synonyms: FMF; pyrin; TRIM20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220043 representing NM_001161790
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGACCCTTGGTGATCATCTGCTAAACACCCTGGAGGAGCTGCTGCCCTATGACTTTGAGAAGT
  TCAAGTTTAAGCTACAGAACACCAGCCTGGAGAAGGGCCACTCTAAGATCCCCCGGGGCCATATGCAGAT
  GGCCAGGCCAGTAAAGCTGGCCAGCCTTCTGATAACCTACTACGGGGAGGAGTATGCTGTAAGGCTGACC
  CTGCAGATCCTGCGGGCCACCAACCAGCGCCAACTAGCAGAGGAGCTTCGCAAAGCCACAGGCACAGAAC
  ATTTAATTGAAGAAAATAGAGTTGGTGGTTCTGTCCAGTCTTCTGTAGAGAATAAAGCCAAGAGTGTGAA
  GGTACCAGATGTCCCTGAAGGTGATGGGACACAGCAAAACAATGATGAATCAGACACCCTTCCATCCAGC
  CAGGCTGAAGTGGGGAAGGGACCCCAGAAGAAGTCACTGACCAAAAGGAAGGATCAGAGGGGCCCCGAGA
  GCCTGGACTCACAGACCAAGCCATGGACAAGGAGCACAGCGCCTCTCTATAGGAGAACCCAGGGCACCCA
  GTCCCCAGGGGATAAGGAGAGCACAGCAAGTGCCCAGCTCCGCAGGAATGTCAGCTCTGCAGGGAGGCTT
  CAAGGACTTTACAACAATGCCCCAGGGAGGAGAGAAAGCAAGAAAGCCGAAGTGTATGTATATTTGCCTT
  CAGGAAAGAAGCGACCCAGGAGTCTTGAAATTACCACTTATTCAAGAGAAGGAGAACCCCCAAATTCAGA
  AGTTCTTCCAACTCAAGAGGAAACAAGAAATGGGAGTCTCATTCGCATGAGAACAGCTACTCTGAATGGA
  AGGACTACGGGGGCTTTAGAAAAGGGCACAGGGATTCCAGAGCATTCCATGGTGCTGGATGAAAAAACAT
  TCAGAAACATGTCTTCCAAAACATCGTTGATTGGGGAGGAGAGATGCCCTACATCATGGACGGAAAATGG
  AAATGGGAGTCCAGAAACCACAGAGTCCTCGGGAGAGACGGCTGGCAGCATACTCTCTGATCCAGAAGTG
  CCTTTGTCACTATGTGAAAAACCAGCTAAAACTCCAGAAGACCCAGCATCCTTAGGACAGGCCGCTTGTG
  AAGGTACCCTCCTTCAGGTCTCAAGAAAATGTGATGCAGGCCCAGAAAAATGCACATTTACACATCTTGC
  ACTACAGGTTGCATCAAATTTCGGAAGGTCACAGGACAAGGCTGTGTGCCCTCTTTGCCACACCCAGGAA
  GGAGACCTGCGTGGTGATACCTGTGTGCAAAGTTCCTGTAGCTGCTCCATTGCTCCTGGGGATCCCAAGG
  CCTCCGGCAGATGCTCCATATGCTTTCAGTGCCAAGGCTTACTTGCCAGAAAGAGCTGCGAAGCCCAGAG
  CCCCCAGTCCCTACCACAGTGCCCTCGTCACATGAAGCAGGTGCTGCTGCTTTTCTGTGAGGACCACAGG
  GAGCCCATCTGCCTCATCTGCAGGCTGAGCCTGGAGCATCAAGGACATCGAGTGCGCCCCATAGAGGAGG
  CTGCACTAGAGTACAAGGAGCAGATCCGGGAGCAGCTGGAGCGCCTGCGGGAGATGAGGGGATATGTGGA
  GGAGCACAGGCTACAGGGAGACAAGAAAACAGACGATTTCCTGAAACAAACAGAAATCCAGAAGCAGAAG
  ATTTCATGTCCACTTGAGAAGCTATACCAACTCCTGGAGAAGCAAGAACAACTCTTTGTGACCTGGCTAC
  AGGAGCTAAGCCAGACCATCAGCAAGGTCAGAGAGACATACTACACCCGGGTTTCCCTACTGGATGAGAT
  GATTGAAGAACTAGAGGCCAAGCAAGACCAGCCAGAGTGGGACCTCATGCAGGACATTGGAATCACCTTG
  CACAGGGCTAAGATGATGTCTGCCTCTGAGCTGTTGGACACTCCTCCAGGTGTTAAAGAAAAGCTTCATC
  TGCTCTACCAGAAGTCAAAGTCTGTGGAGAAGAATATGCAGTGTTTCTCAGAAATGCTGAGCTCTGAAAT
  GGCATTCAGCGCTTCAGATGTAGCAAAATGGGAAGGGCGCCAGCCTAGCGCCACACAGGTCCAGGGCTTG
  GTTCCCACCGTTCACCTAAAGTGTGATGGTGCACACACACAGGACTGTGATGTAGTATTTTACCCAGAAC
  GGGAAGCTGGAGGATCAGAACCTAAAGATTACCTTCATCCTCAGCCAGCTCAAGACACACCTGAGCTACA
  TGAGATCCATTCTCGGAACAACAAAAGAAAATTTAAATCTTTCCTGAAATGGAAACCTTCATTCATTGGG
  AGCACCTTGGGAGAAGCCCACAGATCCGCACTCCCAGCTAGGCATTCTTCTTCCTGCAGTTACCGTGATT
  TGGACCAAGCAGCTGCTCACCCCAACCTCATCTTCTCGATGATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220043 representing NM_001161790
  Red=Cloning site Green=Tags(s)
  
  MAKTLGDHLLNTLEELLPYDFEKFKFKLQNTSLEKGHSKIPRGHMQMARPVKLASLLITYYGEEYAVRLT
  LQILRATNQRQLAEELRKATGTEHLIEENRVGGSVQSSVENKAKSVKVPDVPEGDGTQQNNDESDTLPSS
  QAEVGKGPQKKSLTKRKDQRGPESLDSQTKPWTRSTAPLYRRTQGTQSPGDKESTASAQLRRNVSSAGRL
  QGLYNNAPGRRESKKAEVYVYLPSGKKRPRSLEITTYSREGEPPNSEVLPTQEETRNGSLIRMRTATLNG
  RTTGALEKGTGIPEHSMVLDEKTFRNMSSKTSLIGEERCPTSWTENGNGSPETTESSGETAGSILSDPEV
  PLSLCEKPAKTPEDPASLGQAACEGTLLQVSRKCDAGPEKCTFTHLALQVASNFGRSQDKAVCPLCHTQE
  GDLRGDTCVQSSCSCSIAPGDPKASGRCSICFQCQGLLARKSCEAQSPQSLPQCPRHMKQVLLLFCEDHR
  EPICLICRLSLEHQGHRVRPIEEAALEYKEQIREQLERLREMRGYVEEHRLQGDKKTDDFLKQTEIQKQK
  ISCPLEKLYQLLEKQEQLFVTWLQELSQTISKVRETYYTRVSLLDEMIEELEAKQDQPEWDLMQDIGITL
  HRAKMMSASELLDTPPGVKEKLHLLYQKSKSVEKNMQCFSEMLSSEMAFSASDVAKWEGRQPSATQVQGL
  VPTVHLKCDGAHTQDCDVVFYPEREAGGSEPKDYLHPQPAQDTPELHEIHSRNNKRKFKSFLKWKPSFIG
  STLGEAHRSALPARHSSSCSYRDLDQAAAHPNLIFSMI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161790
• ORF Size: 2424 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161790.1, NP_001155262.1
• RefSeq Size: 3249
• RefSeq ORF: 2427
• Locus ID: 54483