Ap2b1 (NM_027915) Mouse Tagged ORF Clone

CAT#: MG220114

Ap2b1 (GFP-tagged) - Mouse adaptor-related protein complex 2 beta 1 subunit (Ap2b1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ap2b1
• Synonyms: 1300012O03Rik; AI788979; AP105B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220114 representing NM_027915
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACTCCAAGTACTTCACAACCAATAAGAAGGGAGAAATTTTTGAATTAAAAGCTGAACTCAACA
  ATGAAAAGAAAGAAAAGAGGAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGACGT
  GAGCTCTCTCTTCCCAGATGTGGTGAACTGCATGCAGACTGACAACCTGGAACTAAAGAAGCTCGTGTAC
  CTCTATCTGATGAACTATGCCAAGAGTCAGCCAGACATGGCCATCATGGCTGTCAACAGCTTTGTGAAGG
  ATTGTGAAGATCCCAATCCTTTGATTCGAGCCTTGGCAGTTAGAACCATGGGATGCATCCGGGTGGACAA
  GATTACAGAGTATCTCTGTGAACCCCTCCGCAAGTGCTTGAAGGATGAAGACCCCTATGTTCGGAAAACG
  GCAGCAGTATGCGTGGCAAAACTCCATGATATCAATGCCCAGATGGTGGAAGATCAGGGATTTCTGGATT
  CTCTGCGGGATCTCATAGCAGATTCAAACCCAATGGTGGTGGCTAATGCTGTAGCAGCATTGTCTGAGAT
  CAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCTCAGAATATCAATAAGCTGCTCACAGCC
  CTGAATGAATGCACTGAATGGGGCCAGATTTTCATCCTGGACTGCCTGTCTAATTACAACCCTAAAGATG
  ACCGGGAAGCTCAGAGCATCTGTGAGCGAGTAACGCCTCGGCTCTCCCACGCCAACTCTGCAGTGGTGCT
  TTCAGCCGTAAAAGTCCTAATGAAGTTTCTAGAATTGTTGCCCAAGGACTCTGACTACTACAATATGCTG
  CTAAAGAAGTTAGCACCTCCACTTGTCACGTTGCTGTCTGGGGAGCCAGAAGTACAGTACGTCGCCCTGA
  GGAACATCAACCTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAAATCAAGGTCTTCTTTGTGAA
  GTACAATGATCCTATCTATGTTAAACTAGAGAAGTTAGACATCATGATTCGTCTTGCATCCCAAGCCAAC
  ATTGCTCAGGTTCTGGCGGAGCTGAAGGAATATGCCACTGAAGTTGATGTGGACTTTGTTCGCAAAGCTG
  TGAGGGCCATTGGACGGTGTGCCATCAAAGTGGAGCAATCAGCAGAACGCTGTGTAAGCACACTGCTTGA
  TCTAATCCAGACCAAAGTAAATTATGTGGTCCAAGAGGCAATTGTTGTCATCAGGGACATCTTCCGAAAA
  TACCCCAACAAGTATGAAAGTATTATTGCCACCCTCTGTGAAAACTTGGACTCCCTGGATGAACCCGATG
  CCCGAGCAGCTATGATTTGGATTGTAGGAGAATATGCTGAAAGAATCGATAACGCAGATGAATTACTAGA
  GAGTTTCCTGGAAGGTTTTCATGACGAAAGCACCCAGGTGCAGCTCACACTGCTCACCGCCATAGTGAAG
  CTGTTTCTCAAGAAGCCATCAGAAACACAGGAGCTGGTCCAACAGGTCTTGAGCTTAGCCACACAGGACT
  CCGATAACCCTGACCTTCGAGATCGGGGTTATATATATTGGCGCCTTCTTTCAACTGACCCTGTGACAGC
  CAAAGAAGTGGTGTTGTCTGAGAAGCCATTGATCTCTGAGGAGACAGACCTCATTGAGCCTACCCTCCTG
  GATGAGCTCATCTGCCACATTGGTTCTTTGGCCTCTGTGTACCACAAACCTCCAAATGCTTTTGTGGAAG
  GGAGTCATGGGATTCATCGCAAACACTTGCCAATTCATCATGGGAGCACTGATGCAGGTGATAGCCCCGT
  TGGCACCACCACTACAACCAACCTGGAGCAGCCTCAGGTCATTCCCTCTCAAGGTGACCTTCTGGGGGAT
  CTTTTGAATCTTGACCTGGGTCCCCCAGTGAATGTCCCGCAAGTGTCCTCCATGCAGATGGGAGCGGTGG
  ATCTTTTAGGAGGAGGACTGGATAGCCTGGTAGGACAGTCCTTCATCCCGTCATCAGTGCCTGCGACCTT
  CGCTCCTTCACCTACTCCTGCTGTGGTCAGCAGTGGTCTGAATGACCTGTTTGAGCTTTCCACTGGGATA
  GGCATGGCACCTGGCGGATATGTGGCTCCTAAGGCGGTCTGGCTACCTGCAGTAAAGGCTAAAGGCTTGG
  AGATTTCGGGGACGTTTACTCACCGCCAAGGGCATATCTATATGGAAATGAACTTCACCAACAAAGCTCT
  GCAGCATATGACAGACTTTGCCATCCAGTTTAACAAGAATAGCTTCGGTGTCATCCCAAGCACTCCCTTG
  GCCATACATACTCCACTGATGCCAAACCAGAGCATTGACGTCTCTCTGCCGCTCAACACCTTGGGCCCAG
  TCATGAAGATGGAGCCCCTGAATAACCTGCAGGTGGCTGTTAAAAACAATATTGATGTCTTCTACTTCAG
  CTGCCTCATCCCACTCAATGTGCTTTTTGTAGAAGATGGCAAAATGGAGCGCCAGGTCTTCCTTGCAACG
  TGGAAGGATATTCCCAATGAAAATGAACTCCAGTTTCAGATTAAGGAATGTCATTTAAATGCTGACACAG
  TTTCCAGCAAGTTGCAAAACAACAATGTTTACACTATTGCCAAGAGGAACGTGGAAGGGCAGGACATGCT
  GTACCAGTCCCTGAAGCTCACTAATGGCATTTGGATTTTGGCAGAGCTGCGGATCCAGCCAGGAAACCCC
  AATTATACGCTGTCGCTGAAGTGTAGAGCCCCTGAAGTCTCTCAGTACATCTATCAGGTCTACGACAGCA
  TTTTGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220114 representing NM_027915
  Red=Cloning site Green=Tags(s)
  
  MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVY
  LYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT
  AAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTA
  LNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNML
  LKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQAN
  IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRK
  YPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVK
  LFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLL
  DELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTTTNLEQPQVIPSQGDLLGD
  LLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLVGQSFIPSSVPATFAPSPTPAVVSSGLNDLFELSTGI
  GMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQFNKNSFGVIPSTPL
  AIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLFVEDGKMERQVFLAT
  WKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNGIWILAELRIQPGNP
  NYTLSLKCRAPEVSQYIYQVYDSILKN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027915
• ORF Size: 2811 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027915.3, NP_082191.1
• RefSeq Size: 5369
• RefSeq ORF: 2814
• Locus ID: 71770