Nfasc (NM_001160317) Mouse Tagged ORF Clone

CAT#: MG220935

Nfasc (GFP-tagged) - Mouse neurofascin (Nfasc) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nfasc
• Synonyms: AA387016; D430023G06Rik; mKIAA0756; NF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220935 representing NM_001160317
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACATAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATCGAGATTCCGATGGACCTGACCCAACCCCCAACTATCACCAAGCAGTCCGTGAAGGACCACATCGT
  GGACCCTCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGCAACCCCGCCCCCAGTTTTCACTGGACT
  CGCAACAGCAGATTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGATCTGGGACCTTGG
  TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAATACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA
  ATTTGGCACTGCACTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC
  CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTGACACTACAGTGCAACCCCCCACCCGGCCTCCCGT
  CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA
  CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAACGCGCGC
  TTTCACTTCACCCACACCATTCAGCAGAAGAACCCCTTCACCCTCAAGGTCCTCACCAACAACCCCTATA
  ATGACTCGTCCTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCAGAAAGAACGCCCAGCTT
  CATGTATCCCCAGGGCACATCGAGCAGTCAGATGGTTCTCCGTGGCATGGACCTGCTGCTGGAATGCATT
  GCCTCTGGCGTCCCAACACCAGACATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTAACAAGGCCA
  AGTTCGAGAACTTTAATAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG
  CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCTCCATACTGG
  CTGGATGAGCCCAAGAACCTGATCCTGGCTCCTGGGGAAGATGGGAGGCTGGTATGCCGAGCCAATGGGA
  ACCCGAAGCCGACCGTGCAGTGGATGGTGAATGGAGAGCCTTTACAATCGGCACCACCCAATCCCAACCG
  TGAGGTAGCTGGAGACACTATCATCTTCCGGGATACTCAGATCAGCAGCAGGGCAGTGTACCAATGTAAT
  ACATCCAATGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTGTTAGATGTACCCCCTCGGATGC
  TGTCTGCCCGCAACCAGCTCATCAGGGTGATCCTTTATAACCGGACACGGCTGGACTGTCCGTTCTTTGG
  GTCTCCCATCCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGCGGTAACTACCAC
  GTCTACGAAAACGGCAGTCTAGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG
  CCACCAACATCCTGGGCAAAGCCGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG
  GATGCCCGAGGACCAGGTGGCCAAGAGGGGCACCACGGTGCAGCTGGAGTGCCGCGTGAAACATGACCCC
  TCCTTGAAGCTCACAGTCTCCTGGCTGAAGGACGATGAGCCACTCTACATTGGAAACAGGATGAAGAAGG
  AAGATGACTCCCTGACGATCTTCGGAGTGGCAGAGCGGGACCAGGGCAGTTACACGTGTATGGCCAGCAC
  CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGACCAGACCGACCCAGGGACCTG
  GAGCTCACTGACCTGGCTGAGAGGAGTGTGAGGCTGACCTGGATCCCAGGGGATGACAACAACAGCCCTA
  TCACAGACTACGTCGTTCAGTTTGAAGAGGACCAGTTCCAACCAGGGGTGTGGCATGACCACTCCAGGTT
  CCCAGGCAGCGTCAACTCAGCCGTCCTCCATCTGTCCCCATATGTCAACTACCAATTCAGAGTCATCGCT
  GTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACCAGTGGGGCACCCCCTG
  AATCTAATCCCAGTGATGTGAAGGGCGAAGGGACAAGAAAGAACAATATGGAGATCACGTGGACGCCTAT
  GAATGCTACCTCTGCCTTTGGCCCCAACCTACGCTACATTGTCAAGTGGCGACGGAGAGAGACCCGAGAG
  ACTTGGAACAATGTCACAGTGTGGGGCTCTCGCTACGTGGTGGGGCAGACGCCTGTCTACGTTCCCTATG
  AGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCCGAGCCTGACACCATCATTGGGTACTCCGG
  AGAAGATTATCCCAGGGCTGCGCCCACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTT
  CAGTGGAACCGAGTCTACTCTGACACGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGG
  AAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGTGACCGTCC
  CCGGGGCGTGGTGGCCCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGATGGTGGTGGTCAATGGGAGA
  GGTGACGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCAGAAGGAGTACCCAGTGCCCCCAGGCGGT
  TCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAGTGGGACCACCCAGAGCACCCCAACGGAAT
  CCTGATTGGATACATCCTCAGATACGTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAAC
  TTCTCTCCCAATCAGACCAAGTTCTCTGTGCAGAGAGCAGACCCAGTGTCGCGTTACCGCTTCTCCCTCA
  GTGCCAGGACACAGGTGGGCTCTGGAGAAGCAGCCACAGAGGAGTCCCCAGCACCTCCAAATGAAGCTAC
  TCCAACTGCAGCTTACACCAATAACCAGGCAGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGT
  GCCATTGCCCTTCTGGTGCTGATCCTTCTCATCGTCTGCTTCATCAAGAGGAGTCGAGGTGGCAAGTACC
  CAGTGCGGGAAAAGAAGGATGTCCCCTTGGGTCCTGAAGACCCCAAAGAAGAAGATGGCTCATTTGACTA
  CAGTGATGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGATGGCACCATCAAGCAGCAGGAG
  AGCGATGACAGCCTGGTGGACTATGGCGAAGGCGGCGAGGGCCAGTTCAATGAAGATGGCTCCTTTATTG
  GCCAGTACACTGTCAAAAAGGACAAGGAGGAAACGGAGGGCAATGAGAGCTCAGAGGCCACATCACCAGT
  CAATGCCATCTATTCCCTTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220935 representing NM_001160317
  Red=Cloning site Green=Tags(s)
  
  MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWT
  RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN
  LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR
  FHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECI
  ASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW
  LDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN
  TSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH
  VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDP
  SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDL
  ELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIA
  VNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRE
  TWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISL
  QWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGR
  GDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVEN
  FSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMC
  AIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQE
  SDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160317
• ORF Size: 3522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001160317.1, NP_001153789.1
• RefSeq Size: 9597
• RefSeq ORF: 3525
• Locus ID: 269116
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]