Ralgds (NM_001145834) Mouse Tagged ORF Clone

CAT#: MG220967

Ralgds (GFP-tagged) - Mouse ral guanine nucleotide dissociation stimulator (Ralgds) transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ralgds
• Synonyms: Gnds; mKIAA1308; Rgds
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220967 representing NM_001145834
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGTAGATTGCCAGAGCTCCACGCAGGAGATTGGGGAGGAGCTGATCAACGGGGTCATCTACTCCA
  TCTCCCTGCGCAAGGTCCAGCTACACCAAGGAGCCACTAAGGGCCAGCGCTGGCTAGGGTGTGAGAACGA
  GTCGGCTCTGAACCTCTATGAGACCTGCAAGGTGCGCACGGTGAAGGCTGGTACTCTGGAGAAGCTGGTG
  GAACACCTGGTGCCTGCCTTCCAGGGCAGTGACCTTTCCTACGTCACTGTCTTCCTGTGCACCTACAGAG
  CCTTCACTACCACCCAGCAGGTGCTAGACCTGCTGTTCAAAAGATATGGCTGCATCCTCCCCTACTCCAG
  TGAGGACGGCGGACCGCAGGACCAACTCAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAA
  TACTCAGAGGATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCTTATGTACAGCTCA
  ACATGCCTGGCTCAGATCTGGAGCGCCGCGCTCACCTTCTCCTGGCCCAGCTGGAGGACCTGGAGCCCAG
  TGAGGCTGAGTCTGAGGCCCTGTCCCCAGCTCCAGTGCTGTCTCTGAAGCCAGCTTCACAGCTAGAACCT
  GCACTGCTGCTGACGCCCAGCCAAGTGGTGACATCAACTCCAGTACGAGAGCCCGCTGCGGCCCCAGTGC
  CAGTGCTGGCCTCCAGCCCAGTGGTGGCACCAGCTCCTGAGCTAGAACCAGTTCCAGAGCCACCTCAAGA
  GCCTGAGCCATCCCTAGCACTGGCTCCAGAGCTGGAGCCCGCCGTCTCACAGAGCCTGGAACTGGAGTCA
  GCTCCTGTGCCCACTCCTGCCTTAGAGCCTTCCTGGTCTCTGCCTGAAGCCACGGAGAATGGACTAACCG
  AGAAGCCTCACCTTCTGCTGTTCCCTCCTGACTTGGTGGCTGAACAGTTTACTCTGATGGATGCAGAACT
  ATTCAAGAAAGTCGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAACGGGACAAGAAGGGCAAGGAG
  CACCTCGCGCCTACCATCCGCGCCACTGTCGCCCAGTTCAACAACGTGGCCAACTGTGTCATTACTACCT
  GCCTTGGGGACCAGAGTATGAAGGCTCCGGACAGGGCCCGGGTGGTGGAACACTGGATCGAGGTGGCCAG
  GGAGTGCAGAGCGCTCAAGAATTTCTCCTCCCTCTACGCCATCCTCTCTGCTCTACAGAGCAATGCCATC
  CACCGCCTAAAGAAGACGTGGGAAGAGGTCTCCAGGGACAGCTTTCGAGTGTTCCAGAAACTGTCGGAGA
  TCTTCTCTGATGAGAACAACTACTCCCTGAGCAGAGAGCTGCTCATCAAGGAAGGAACCTCCAAGTTTGC
  CACACTGGAGATGAACCCTAGGAGAGCCCAGAGGCGGCAGAAGGAGACAGGAGTCATCCAGGGCACCGTT
  CCCTACCTGGGCACATTCCTCACTGACCTGGTGATGCTGGACACTGCCATGAAGGACTATCTCTATGGGA
  GACTGATCAACTTTGAAAAGAGAAGGAAGGAGTTCGAAGTCATTGCCCAGATCAAGTTGCTACAGTCAGC
  CTGCAACAACTACAGCATTGCTCCGGAAGAACACTTTGGAACATGGTTCCGAGCTATGGAGCGACTCAGT
  GAGGCTGAGAGCTACACCCTGTCGTGTGAGCTGGAGCCCCCGTCTGAGTCGGCCAGCAACACCCTGAGGA
  GCAAGAAAAGCACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCTCCCAGCACGGAGCTCAGCACCAG
  TAGCAGTGCCCACTCCAAGTCCTGTGACCAGCTTCGGTGCAGCCCTTACCTCGGCAGCGGGGACATCACC
  GACGCGCTCAGTGTGCACTCAGCTGGCTCTTCCAGCTCTGATGTGGAGGAGATCAACATGAGCTTCGTCC
  CAGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAGTCAGCCTCCCAGTCGTCCCCAGAGACCTCTGG
  CATCAGCTCCGCCTCCAGCAGCACCTCCTCTTCGTCAGCCTCCACCACGCCCGTGTCTACCACGCGCACC
  CACAAGCGCTCCGTCTCAGGGGTCTGCAGCTACAGCTCCTCACTGCCTCTCTACAACCAGCAGGTGGGCG
  ACTGCTGCATCATCAGGGTCAGCCTGGATGTGGACAACGGCAACATGTACAAGAGCATCCTGGTGACCAG
  CCAGGATAAGGCTCCGACTGTCATCCGAAAAGCCATGGACAAACACAACCTAGATGAGGACGAGCCGGAG
  GATTATGAGCTGGTGCAGATCATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCT
  ATGCCATGAACTCTACCGCCAACTATGACTTCATCCTAAAGAAGCGGACCTTCACTAAGGGGGCTAAAGT
  CAAGCATGGAGCCAGCTCCACCCTCCCTCGTATGAAGCAGAAGGGACTCAGGATTGCCAAAGGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220967 representing NM_001145834
  Red=Cloning site Green=Tags(s)
  
  MMVDCQSSTQEIGEELINGVIYSISLRKVQLHQGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLV
  EHLVPAFQGSDLSYVTVFLCTYRAFTTTQQVLDLLFKRYGCILPYSSEDGGPQDQLKNAISSILGTWLDQ
  YSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEDLEPSEAESEALSPAPVLSLKPASQLEP
  ALLLTPSQVVTSTPVREPAAAPVPVLASSPVVAPAPELEPVPEPPQEPEPSLALAPELEPAVSQSLELES
  APVPTPALEPSWSLPEATENGLTEKPHLLLFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKE
  HLAPTIRATVAQFNNVANCVITTCLGDQSMKAPDRARVVEHWIEVARECRALKNFSSLYAILSALQSNAI
  HRLKKTWEEVSRDSFRVFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPRRAQRRQKETGVIQGTV
  PYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEHFGTWFRAMERLS
  EAESYTLSCELEPPSESASNTLRSKKSTAIVKRWSDRQAPSTELSTSSSAHSKSCDQLRCSPYLGSGDIT
  DALSVHSAGSSSSDVEEINMSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVSTTRT
  HKRSVSGVCSYSSSLPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPTVIRKAMDKHNLDEDEPE
  DYELVQIISEDHKLKIPENANVFYAMNSTANYDFILKKRTFTKGAKVKHGASSTLPRMKQKGLRIAKGIF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145834
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145834.1, NP_001139306.1
• RefSeq Size: 3741
• RefSeq ORF: 2523
• Locus ID: 19730
• Gene Summary: Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap. [UniProtKB/Swiss-Prot Function]