Grin2d (NM_008172) Mouse Tagged ORF Clone

CAT#: MG220972

Grin2d (GFP-tagged) - Mouse glutamate receptor ionotropic NMDA2D (epsilon 4) (Grin2d), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grin2d
• Synonyms: GluN2D; NMDAR2D; NR2D
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220972 representing NM_008172
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGCGCCGGTGGCCCCCGCGGCCCTCGGGGCCCCGCTAAGATGCTGTTGCTGCTGGCGCTGGCGT
  GCGCCAGCCCGTTCCCGGAGGAGGTGCCGGGGCCGGGCGCGGCCGGCGGGGGCACGGGCGGGGCGCGGCC
  GCTCAACGTGGCGCTGGTCTTCTCTGGCCCGGCGTACGCGGCCGAGGCGGCGCGCTTGGGCCCGGCCGTG
  GCGGCGGCAGTGCGCAGCCCGGGCCTGGACGTACGGCCCGTGGCGCTGGTGCTCAACGGCTCCGACCCTC
  GCAGCCTTGTGCTGCAGCTCTGCGACCTGCTGTCGGGGCTGCGCGTGCACGGCGTGGTGTTCGAGGACGA
  CTCGCGCGCGCCCGCCGTCGCGCCCATTCTCGACTTCCTGTCGGCGCAGACCTCGCTGCCCATCGTGGCC
  GTGCACGGCGGCGCCGCGCTCGTACTCACACCCAAGGAGAAGGGCTCCACCTTCCTGCAGCTTGGCTCCT
  CCACAGAGCAACAGCTGCAGGTCATTTTTGAGGTGCTGGAGGAGTACGACTGGACATCCTTTGTGGCAGT
  GACTACGCGTGCCCCAGGCCATCGAGCCTTCTTGTCATACATCGAGGTGCTGACTGATGGCAGCCTGGTG
  GGCTGGGAGCATCGAGGAGCGCTGACACTGGACCCCGGAGCGGGTGAGGCCGTCCTGGGCGCACAGCTCC
  GTAGTGTCAGTGCGCAGATCCGCCTGCTCTTCTGCGCCCGCGAGGAGGCAGAGCCTGTTTTCCGGGCGGC
  AGAAGAGGCTGGTCTCACTGGGCCTGGCTACGTCTGGTTCATGGTGGGACCTCAGCTGGCCGGAGGTGGG
  GGCTCCGGGGTCCCTGGGGAACCACTTCTTCTGCCAGGAGGTGCCCCACTGCCTGCTGGGCTGTTTGCAG
  TGCGCTCTGCTGGCTGGCGTGACGACTTGGCACGTCGAGTGGCTGCTGGTGTGGCGGTGGTGGCCAGAGG
  TGCCCAGGCCCTGCTGCGAGACTATGGCTTCCTGCCTGAGCTGGGCCATGACTGTCGCGCCCAGAATCGC
  ACCCACCGCGGGGAGAGTCTGCACAGGTATTTCATGAACATCACCTGGGATAACCGAGACTACTCCTTCA
  ATGAGGATGGCTTTCTGGTAAACCCGTCACTGGTAGTCATCTCCCTCACCAGAGACAGGACGTGGGAAGT
  GGTGGGCAGCTGGGAACAGCAGACCCTCCGCCTCAAGTACCCTCTATGGTCCCGCTATGGCCGCTTCCTG
  CAGCCGGTGGATGACACGCAGCACCTCACTGTGGCCACGCTGGAGGAGAGACCTTTTGTCATTGTAGAGC
  CTGCAGACCCCATCAGCGGCACTTGCATCAGAGACTCGGTTCCCTGCCGGAGCCAGCTCAACCGTACCCA
  CAGCCCTCCGCCTGACGCTCCCCGCCCGGAGAAGAGATGCTGCAAGGGTTTCTGCATTGACATTTTGAAG
  AGGCTGGCGCACACCATCGGTTTCAGCTATGACCTCTACCTGGTTACCAACGGCAAGCATGGCAAGAAGA
  TCGATGGCGTCTGGAATGGCATGATTGGTGAGGTGTTCTATCAGCGTGCGGACATGGCCATCGGCTCCCT
  CACCATCAATGAGGAGCGGTCAGAGATCGTGGACTTCTCCGTCCCTTTTGTAGAGACAGGCATCAGCGTC
  ATGGTGGCACGCAGCAATGGCACTGTGTCCCCCTCTGCCTTCCTCGAGCCCTACAGCCCCGCTGTGTGGG
  TGATGATGTTCGTCATGTGCCTCACCGTGGTCGCCGTCACAGTTTTCATCTTTGAGTACCTCAGTCCTGT
  GGGCTATAACCGAAGCCTGGCCACGGGCAAACGCCCCGGAGGCTCTACCTTCACCATTGGGAAATCCATC
  TGGCTGCTGTGGGCCCTGGTGTTCAACAACTCCGTGCCAGTGGAGAATCCTCGGGGCACCACCAGCAAGA
  TCATGGTGCTGGTGTGGGCCTTCTTTGCCGTCATCTTTCTTGCCAGCTATACAGCCAATCTGGCTGCCTT
  CATGATCCAGGAGGAGTACGTGGACACCGTGTCTGGGCTCAGCGACCGGAAGTTCCAGCGGCCCCAGGAG
  CAATACCCACCCCTGAAGTTTGGAACGGTGCCCAATGGGTCCACGGAGAAGAATATCCGAAGCAACTACC
  CTGATATGCACAGCTACATGGTGCGATACAACCAGCCAAGAGTGGAGGAGGCGCTCACTCAGCTCAAGGC
  AGGGAAACTGGACGCCTTCATCTATGACGCAGCAGTGCTCAACTACATGGCCCGAAAGGATGAGGGCTGC
  AAGCTGGTCACCATCGGCTCAGGCAAGGTCTTCGCCACCACTGGTTATGGCATCGCCCTACACAAGGGCT
  CCCGCTGGAAGAGGCCCATCGACCTGGCGCTGCTGCAGTTCCTGGGGGACGATGAGATTGAGATGCTGGA
  GCGGCTGTGGCTTTCAGGGATCTGCCACAACGACAAAATCGAGGTGATGAGCAGCAAGCTGGATATCGAC
  AACATGGCAGGTGTCTTCTACATGCTCCTCGTGGCCATGGGCCTCTCCTTGCTGGTCTTCGCCTGGGAAC
  ACCTTGTGTACTGGCGACTGCGGCACTGTCTGGGGCCCACCCACCGCATGGATTTCCTACTGGCCTTCTC
  CAGGGGTATGTACAGCTGCTGCAGCGCTGAGGCTGCTCCGCCGCCGGCCAAACCCCCGCCACCGCCGCAG
  CCGCTGCCCAGTCCGGCGTATCCCGCCGCTCGCCCACCCCCTGGCCCCGCACCCTTCGTGCCCCGAGAGC
  GCGCAGCCGCCGACCGCTGGCGCCGGGCCAAGGGCACAGGGCCCCCGGGGGGCGCAGCGCTAGCCGACGG
  CTTCCACCGATACTACGGCCCCATCGAGCCGCAGGGGCTGGGCCTCGGCGAGGCGCGCGCGGCACCGAGA
  GGCGCAGCCGGACGCCCACTGTCCCCACCCACCACACAGCCCCCACAGAAGCCACCACCTTCCTACTTCG
  CCATCGTGCGCGAGCAAGAGCCGGCCGAGCCCCCCGCCGGCGCCTTCCCGGGCTTCCCATCTCCGCCCGC
  TCCGCCTGCCGCCGCAGCCGCCGCCGTCGGGCCGCCACTGTGCCGCCTGGCTTTCGAGGACGAGAGCCCG
  CCCGCGCCCTCGCGCTGGCCGCGTTCTGACCCCGAGAGCCAGCCGCTGTTGGGTGGGGGCGCGGGCGGCC
  CGAGCGCTGGGGCCCCGACCGCACCACCGCCGCGCCGCGCCGCGCCACCACCGTGCGCCTACCTGGACCT
  CGAGCCTTCGCCTTCGGACTCCGAGGATTCGGAGAGCCTGGGCGGAGCGTCGCTCGGTGGCCTGGAGCCC
  TGGTGGTTCGCCGACTTCCCCTACCCGTATGCGGAGCGCCTCGGGCCGCCGCCCGGCCGCTACTGGTCGG
  TTGACAAGCTCGGGGGCTGGCGCGCTGGTAGCTGGGACTACCTGCCCCCGCGCGGCGGCCCCGCATGGCA
  CTGCCGCCACTGCGCCAGCCTGGAGCTGCTACCGCCACCACGCCATCTCAGCTGCTCGCACGACGGCCTA
  GACGGTGGCTGGTGGGCGCCTCCGCCTCCACCCTGGGCTGCGGGGCCACCGCCCCGGCGCCGGGCGCGCT
  GTGGTTGTCCGCGACCGCACCCGCACCGCCCCCGGGCTTCGCACCGTGCGCCCGCCGCCGCACCGCACCA
  CCACCGACACAGGCGCGCGGCGGGTGGCTGGGACCTCCCGCCGCCCGCGCCCACCTCGCGTTCGCTGGAG
  GACCTGAGCTCCTGCCCACGGGCGGCCCCCACGCGCAGGCTCACCGGGCCCTCGCGCCACGCGCGCCGCT
  GTCCGCACGCTGCGCATTGGGGGCCGCCCCTGCCCACCGCATCTCACCGGAGACACCGGGGCGGGGACCT
  GGGCACACGCAGGGGCTCTGCGCACTTCTCCAGCCTGGAGTCCGAGGTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220972 representing NM_008172
  Red=Cloning site Green=Tags(s)
  
  MRGAGGPRGPRGPAKMLLLLALACASPFPEEVPGPGAAGGGTGGARPLNVALVFSGPAYAAEAARLGPAV
  AAAVRSPGLDVRPVALVLNGSDPRSLVLQLCDLLSGLRVHGVVFEDDSRAPAVAPILDFLSAQTSLPIVA
  VHGGAALVLTPKEKGSTFLQLGSSTEQQLQVIFEVLEEYDWTSFVAVTTRAPGHRAFLSYIEVLTDGSLV
  GWEHRGALTLDPGAGEAVLGAQLRSVSAQIRLLFCAREEAEPVFRAAEEAGLTGPGYVWFMVGPQLAGGG
  GSGVPGEPLLLPGGAPLPAGLFAVRSAGWRDDLARRVAAGVAVVARGAQALLRDYGFLPELGHDCRAQNR
  THRGESLHRYFMNITWDNRDYSFNEDGFLVNPSLVVISLTRDRTWEVVGSWEQQTLRLKYPLWSRYGRFL
  QPVDDTQHLTVATLEERPFVIVEPADPISGTCIRDSVPCRSQLNRTHSPPPDAPRPEKRCCKGFCIDILK
  RLAHTIGFSYDLYLVTNGKHGKKIDGVWNGMIGEVFYQRADMAIGSLTINEERSEIVDFSVPFVETGISV
  MVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAVTVFIFEYLSPVGYNRSLATGKRPGGSTFTIGKSI
  WLLWALVFNNSVPVENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEEYVDTVSGLSDRKFQRPQE
  QYPPLKFGTVPNGSTEKNIRSNYPDMHSYMVRYNQPRVEEALTQLKAGKLDAFIYDAAVLNYMARKDEGC
  KLVTIGSGKVFATTGYGIALHKGSRWKRPIDLALLQFLGDDEIEMLERLWLSGICHNDKIEVMSSKLDID
  NMAGVFYMLLVAMGLSLLVFAWEHLVYWRLRHCLGPTHRMDFLLAFSRGMYSCCSAEAAPPPAKPPPPPQ
  PLPSPAYPAARPPPGPAPFVPRERAAADRWRRAKGTGPPGGAALADGFHRYYGPIEPQGLGLGEARAAPR
  GAAGRPLSPPTTQPPQKPPPSYFAIVREQEPAEPPAGAFPGFPSPPAPPAAAAAAVGPPLCRLAFEDESP
  PAPSRWPRSDPESQPLLGGGAGGPSAGAPTAPPPRRAAPPPCAYLDLEPSPSDSEDSESLGGASLGGLEP
  WWFADFPYPYAERLGPPPGRYWSVDKLGGWRAGSWDYLPPRGGPAWHCRHCASLELLPPPRHLSCSHDGL
  DGGWWAPPPPPWAAGPPPRRRARCGCPRPHPHRPRASHRAPAAAPHHHRHRRAAGGWDLPPPAPTSRSLE
  DLSSCPRAAPTRRLTGPSRHARRCPHAAHWGPPLPTASHRRHRGGDLGTRRGSAHFSSLESEV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008172
• ORF Size: 3969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008172.2, NP_032198.2
• RefSeq Size: 4383
• RefSeq ORF: 3972
• Locus ID: 14814
• Gene Summary: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. [UniProtKB/Swiss-Prot Function]