Dgki (NM_001081206) Mouse Tagged ORF Clone

CAT#: MG221210

Dgki (GFP-tagged) - Mouse diacylglycerol kinase iota (Dgki), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dgki
• Synonyms: 6430604M11Rik; C130010K08Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221210 representing NM_001081206
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTGCGGGAAGGGGCTGCCATTTGCTGCCCCTGCCAGCGGCGCGCGGACCTGCCCGCGCTCCCG
  CCGCCTCCAGCGCCCTCAGCCCGACCGGCCTCTGCAGCGGCACCACCTCGGCTTCTTTCGCGGCCGCCGG
  AGCGGTCGCCATGAACCCCAGCTCCTCGGCGGGAGAGGAGAGAGGGGCGACGGGTGGCAGCAGCAGCAGC
  GGAAGCGGCGCCGGGAGCTGCTGCCTGGGCGCCGAGGGCGGCGCGGACCCGCGGGGTGCAGGGGCAGCGG
  CTGCTGCTGCCTTGGAGGAGCCCGCGGCCGCCGGACAGAAAGAGAAGGAAGAGGCGCTGGAGGAGAAGCT
  GAGGGACTTAACTTTCCGGAAGCAGGTCTCTTACAGGAAAGCCATCTCCCGGACAGGCCTCCAGCATCTG
  GCACCTGCACATCCTCTTGGTCTTCCTGTGGCTAACGGTCCAGCGAAGGAGCCCAGAGCCACTCTGGACT
  GGAGCGAGAATGCTGTGAATGGGGAGCACCTGTGGCTGGAGACCAACGTTTCAGGGGACCTCTGCTACCT
  GGGGGAGGAGAACTGCCAAGTCCGATTTGCAAAATCCGCTCTCAGGAGGAAGTGTGCCGTCTGCAAAATC
  GTGGTCCACACAGCCTGCATTGAGCAACTAGAGAAGATTAATTTCAGATGTAAACCAACATTTCGGGAAG
  GAGGCTCCAGATCGCCAAGAGAAAACTTTGTGCGTCACCACTGGGTCCACAGGAGGCGGCAGGAGGGAAA
  ATGTAAGCAGTGTGGTAAGGGCTTCCAGCAAAAGTTCTCCTTCCACAGTAAAGAGATCGTGGCCATCAGC
  TGTTCTTGGTGCAAGCAGGCGTTTCACAATAAAGTGACCTGCTTCATGCTGCATCATATTGAGGAACCCT
  GCTCCCTGGGGGCTCACGCCGCTGTTATTGTCCCGCCCACCTGGATCATTAAGGTGAAGAAACCTCAGAA
  CTCCCTGAAGGCTTCCAACCGGAAGAAGAAGAGGACAAGTTTTAAAAGGAAAGCCAGTAAAAGAGGAACT
  GAGCAGGAAACCAAAGGCCGTCCTTTTGTGATCAAGCCCATCTCCTCCCCTCTCATGAAGCCTTTGCTTG
  TCTTTGTGAACCCAAAGAGTGGGGGAAACCAGGGAACCAAAGTCCTACAGATGTTTATGTGGTACCTGAA
  TCCACGGCAAGTCTTTGATCTTTCTCAGGAAGGGCCAAAAGATGCGCTGGAGATGTACAGGAAGGTACCA
  AATCTGAGAATTCTGGCCTGCGGTGGGGATGGAACGGTGGGCTGGATCCTCTCCATCCTGGACGAGCTGC
  AGCTGAGCCCACAGCCTCCTGTGGGGGTGCTTCCACTGGGGACTGGGAATGACCTGGCTCGGACGCTCAA
  CTGGGGAGGGGGTTACACCGATGAGCCTGTTTCTAAGATCCTGTGCCAGGTGGAAGATGGGACCATTGTA
  CAGTTGGATCGCTGGAACCTCCACGTAGAGAGAAACCCAGACCTGCCGCCCGAAGAACTTGAAGATGGCG
  TGTGTAAGCTTCCTCTGAATGTCTTCAATAATTACTTCAGCCTTGGATTTGATGCTCACGTCACATTGGA
  GTTCCATGAATCCCGAGAAGCAAATCCAGAGAAATTCAATAGTCGTTTTCGAAATAAAATGTTCTATGCA
  GGGGCAGCCTTTTCTGATTTCCTACAAAGAAGTTCTAGAGATCTATCCAAACACGTCAAAGTTGTCTGTG
  ATGGGACAGACCTCACCCCAAAGATCCAGGACCTGAAGTTCCAGTGTATAGTATTTTTAAATATTCCCAG
  ATATTGTGCTGGCACAATGCCTTGGGGAAACCCTGGAGATCATCATGACTTTGAACCCCAACGTCATGAT
  GACGGCTACATTGAAGTCATTGGATTCACTATGGCCTCTTTGGCTGCCCTGCAAGTTGGGGGGCACGGAG
  AAAGGCTGCACCAGTGTCGGGAAGTGATGCTTCTGACTTACAAGTCCATTCCCATGCAAGTGGACGGGGA
  GCCATGTCGGTTGGCACCTGCTATGATTCGGATCTCCTTGAGGAATCAGGCCAACATGGTACAGAAGAGC
  AAGCGGAGAACCTCGATGCCTTTGCTCAATGACATCCATCAGGTGCAAGCTGCGGACCTGCGGCGAGTGT
  CTGCTCCCCCCGGCTCCTTCACCATTCCCCAGTCTGTCCCAGATCGCCTGAGGATCCGTGTGAACAAAAT
  CAGTTTACAAGACTATGAAGGACTCCACTATGACAAGGACAAACTCCGGGAAGCTTCCATCCCTCTGGGT
  ATTCTAGTTGTTCGTGGAGACTGTGATTTGGAGACTTGCCGTATGTACATAGACCGTCTACAGGAGGACT
  TGCAGTCAGTTTCTTCTGGGTCACAGAGAGTTCATTACCAGGACCAAGAAACCTCCTTCCCCAGGGCACT
  CTCAGCTCAGAGGCTGTCCCCACGGTGGTGCTTCCTAGATGCAACGTCTGCTGACCGCTTTTATCGAATA
  GACAGATCTCAGGAACATTTGCACTTTGTGATGGAGATTTCTCACGATGAAATTTTTATTCTGGACCCAG
  ATATGGTGGTGTCACAGCAGGCAGGGACCCCTCCAGGAATGCCTGACCTGGTGGTTGAACAGGCTTCTGG
  GCTGTCTGACTGGTGGAACCCTGCCCTGAGGAAACGTATGCTGAGTGACAGTGGGATGATCACACCCCAC
  TATGAGGACTCAGATCTGAAAGACTTCAGCCACTCCAGAGTGCTACAGTCCCCAGTCTCTTCAGAAGATC
  ATGCAATTTTGCAGGCAGTCCTCACCGGTGACCTTATGAAGCTCATGGAGAGCTATAAGAATGGAGGGAG
  CTTACTAATTCAGGGACCAGGGCACTGTTCTCTCCTTCACTACGCAGCTAAGACCGGCAATGGGGACATT
  GTGAAGTACATCCTTGACCACGGACCTGCAGAATTACTGGATATGGCAGACAGTGAAACGGGCGAGACTG
  CACTGCACAAGGCTGCCTGCCAAAGGAACCGGGCTGTATGCCAGCTTCTGGTGGATGCAGGAGCATCTCT
  GAGACAGACAGACTCCAAGGGCAAGACACCCCAGGAACGAGCACAGCAGGCCGGGGACCCAGATTTGGCT
  GCTTACCTAGAAAGCCGACAGAACTATAAGATCATTGGCCATGAAGACCTGGAAACTGCTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221210 representing NM_001081206
  Red=Cloning site Green=Tags(s)
  
  MDAAGRGCHLLPLPAARGPARAPAASSALSPTGLCSGTTSASFAAAGAVAMNPSSSAGEERGATGGSSSS
  GSGAGSCCLGAEGGADPRGAGAAAAAALEEPAAAGQKEKEEALEEKLRDLTFRKQVSYRKAISRTGLQHL
  APAHPLGLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKI
  VVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAIS
  CSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGT
  EQETKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALEMYRKVP
  NLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIV
  QLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYA
  GAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQDLKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHD
  DGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKS
  KRRTSMPLLNDIHQVQAADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGLHYDKDKLREASIPLG
  ILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDQETSFPRALSAQRLSPRWCFLDATSADRFYRI
  DRSQEHLHFVMEISHDEIFILDPDMVVSQQAGTPPGMPDLVVEQASGLSDWWNPALRKRMLSDSGMITPH
  YEDSDLKDFSHSRVLQSPVSSEDHAILQAVLTGDLMKLMESYKNGGSLLIQGPGHCSLLHYAAKTGNGDI
  VKYILDHGPAELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRQTDSKGKTPQERAQQAGDPDLA
  AYLESRQNYKIIGHEDLETAV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081206
• ORF Size: 3213 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001081206.1, NP_001074675.1
• RefSeq Size: 4598
• RefSeq ORF: 3216
• Locus ID: 320127