Enpp7 (NM_001030291) Mouse Tagged ORF Clone

CAT#: MG221425

Enpp7 (GFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 7 (Enpp7), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Enpp7
• Synonyms: Alk-SMase; Gm254
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221425 representing NM_001030291
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACACTCAGCTGTCCTTCTCTGTGTGGCTCTGGCCATTCTTCCAGCTTGTGTCACTGGGGCCCCTG
  TCCAAAGACAGCATAAGCTACTCCTCGTATCCTTTGACGGATTCCGCTGGAACTATGACCAAGACGTGGA
  CACCCCCAACCTGGACTCCATGGCTCAGGAAGGGGTAAAAGCCCAGTATATGACTCCTGCCTTTGTCACC
  ATGACCAGCCCCTGCCACTTTACACTGGTCACCGGCAAATACATCGAGAACCATGGGGTGGTCCACAACA
  TGTTCTACAATACCACCAGCACGGTGAGGCTGCCCTACCACGCCACGCTCGGTATCCAGAGGTGGTGGGA
  CAACGGCAGCATACCCATCTGGATCACAGCCCAGAGGCAGGGCTTAAAAACCGGCTCCTTCTTCTACCCC
  GGTGGGAACGTCACCTACCAAGGAGAGGCCGTAACGATGAGCCGGAAGGAGGGCGTCTTACACAATTACA
  AAAATGAGACGGAGTGGAGGGGGAATGTGGACACGGTGATGAAGTGGTTCCTGGAAGAAGATGTGTCCCT
  GGTCACTCTCTACTTTGGGGAGCCAGACTCCACTGGCCACAAGTATGGCCCTGAGTCCCAGGAGAGGAAG
  GACATGGTGAAGCAGGTAGACAGGACTGTGGGCTACCTCCGGGACAGCATCAAGCGCCACCACCTCTCCG
  ACAGCCTCAACCTGATTATCACATCGGACCACGGTATGACAACCGTCAACAAGAAGGCTAGTGATCTGGT
  GGAGTTCCACAAGTTCTCCAACTTCACCTTCCAGGACATCCAGTTTGAGCTGTTGGACTATGGGCCTATC
  GGGATGCTGATACCCAAGGAAGGGATGCTGGAGAAAGTTTACAGTGTCCTGAAGGACGCCCACCCCAGGC
  TGCATGTGTACAAGAAGGAGGACTTCCCGAAGAACTTTCACTACGCCAACAACCCCAGGATCACACCTCT
  GCTCATGTACAGTGACCTCGGCTATGTTATCCACGGGAGAGTGAATGTCCAGTTCAACAATGGTGAACAT
  GGCTTCAACAACCAAGACATGGACATGAAGACCATCTTCCGGGCCGTGGGTCCCAGCTTCAAGGCAGGCT
  TGGAGGTGGAACCCTTCGAGAGTGTCCATGTGTATGAGCTCATGTGCCAGTTGCTGGGCATTGTGCCCGA
  GCCCAACGATGGGAATCCTGGCATCCTGCGACCCATGCTCCGGTCAGGGTCCGCGTCCTTGCTCAGCAGT
  CAGCACCACTTGGTGGCGTTGTTGTGCAGGAGCCAGAACACAGCAGCATCTGGATTCTACCAAGCTGGTG
  ACTCCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221425 representing NM_001030291
  Red=Cloning site Green=Tags(s)
  
  MGHSAVLLCVALAILPACVTGAPVQRQHKLLLVSFDGFRWNYDQDVDTPNLDSMAQEGVKAQYMTPAFVT
  MTSPCHFTLVTGKYIENHGVVHNMFYNTTSTVRLPYHATLGIQRWWDNGSIPIWITAQRQGLKTGSFFYP
  GGNVTYQGEAVTMSRKEGVLHNYKNETEWRGNVDTVMKWFLEEDVSLVTLYFGEPDSTGHKYGPESQERK
  DMVKQVDRTVGYLRDSIKRHHLSDSLNLIITSDHGMTTVNKKASDLVEFHKFSNFTFQDIQFELLDYGPI
  GMLIPKEGMLEKVYSVLKDAHPRLHVYKKEDFPKNFHYANNPRITPLLMYSDLGYVIHGRVNVQFNNGEH
  GFNNQDMDMKTIFRAVGPSFKAGLEVEPFESVHVYELMCQLLGIVPEPNDGNPGILRPMLRSGSASLLSS
  QHHLVALLCRSQNTAASGFYQAGDSN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001030291
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030291.1, NP_001025462.1
• RefSeq Size: 1370 bp
• RefSeq ORF: 1341 bp
• Locus ID: 238011
• Cytogenetics: 11 E2