Ank1 (NM_031158) Mouse Tagged ORF Clone

CAT#: MG221570

Ank1 (GFP-tagged) - Mouse ankyrin 1 erythroid (Ank1) transcript variant 2, (10ug). Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ank1
• Synonyms: Ank-1; nb; pale
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221570 representing NM_031158, codon optimized.
  Due to the complexity of NM_031158, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGCGGCCGCGCAGGTCGGGGTCAGACCCCGCGGCCGATGCTGCTACCAGCTTTCTGCGGGCGG
  CACGCTCGGGGAACCTGGACAAGGCTCTGGATCACCTGCGCAATGGAGTGGACATTAACACCTGTAACCA
  GAACGGGTTGAACGGCCTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGGTGGTTGAACTTCTGCAC
  AAAGAGATCATTCTAGAAACGACAACCAAGAAGGGGAACACTGCTCTGCACATCGCTGCCCTTGCTGGTC
  AGGATGAGGTGGTCCGGGAGCTGGTCAACTATGGAGCCAATGTCAATGCCCAGTCTCAGAAAGGCTTTAC
  TCCCCTGTACATGGCTGCTCAGGAGAACCACTTGGAAGTGGTGAAATTTCTACTGGAGAATGGAGCCAAT
  CAGAATGTAGCCACAGAAGATGGCTTCACCCCACTGGCCGTGGCTCTACAGCAGGGTCACGAGAATGTGG
  TGGCTCACCTCATCAACTATGGGACGAAAGGGAAAGTGCGCCTCCCTGCCCTGCACATCGCGGCCCGCAA
  CGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGATGTGCTTTCCAAGACGGGA
  TTCACACCCCTCCACATCGCAGCTCACTATGAGAACCTCAACGTGGCCCAGTTGCTCCTCAACAGGGGAG
  CCAGCGTCAACTTCACACCTCAGAATGGCATCACCCCACTACACATCGCCTCCCGCAGGGGGAACGTGAT
  CATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAGGATGAATTGACACCGCTC
  CACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACCACGGGGCACCCATCCAAG
  CCAAAACCAAGAATGGCTTGTCCCCAATCCACATGGCCGCTCAGGGAGACCACCTCGACTGTGTCCGACT
  TCTATTGCAATACAATGCAGAGATAGACGACATCACCTTGGATCACCTGACTCCTCTCCATGTGGCAGCC
  CACTGTGGCCACCACCGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGCCCAACTCCAGAGCCCTGA
  ATGGTTTTACCCCGTTACACATCGCCTGCAAGAAGAACCACATCCGTGTAATGGAGTTGCTGCTGAAGAC
  AGGAGCCTCCATCGACGCGGTCACTGAGTCTGGCCTGACACCTCTCCACGTAGCCTCCTTCATGGGACAC
  CTTCCTATTGTGAAGAACTTACTGCAGCGGGGAGCGTCACCCAATGTCTCCAATGTGAAAGTAGAAACCC
  CCTTGCACATGGCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTCCAGAACAAAGCCAAAGC
  CAACGCCAAGGCCAAGGATGACCAGACACCGCTTCACTGTGCTGCTCGAATCGGCCACACAGGCATGGTG
  AAGCTCCTGCTGGAGAATGGTGCCAGCCCCAATCTGGCTACCACTGCTGGCCACACACCCCTACACACCG
  CAGCCCGTGAGGGACACGTGGACACAGCCCTGGCCCTGCTGGAGAAGGAGGCATCCCAAGCCTGCATGAC
  CAAGAAAGGATTTACCCCTCTGCACGTGGCTGCTAAGTACGGGAAGGTACGGTTGGCGGAGCTGCTGCTG
  GAACACGATGCACACCCCAATGCAGCTGGGAAGAACGGCTTGACTCCTCTGCATGTGGCCGTCCATCACA
  ACAACCTGGACATTGTCAAACTTCTTCTTCCCCGAGGTGGCTCCCCCCACAGCCCTGCCTGGAATGGCTA
  CACTCCTTTGCACATCGCTGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTCTACTGCAGTACGGAGGG
  TCAGCGAATGCAGAGTCGGTACAAGGCGTGACCCCACTTCACCTGGCTGCCCAAGAGGGCCACACAGAAA
  TGGTTGCTCTTCTCCTGTCCAAGCAAGCCAACGGCAACCTGGGGAACAAGAGTGGACTCACTCCCCTCCA
  CCTGGTGTCACAAGAAGGCCATGTTCCAGTGGCAGACGTGCTGATCAAACATGGCGTCACCGTGGATGCT
  ACCACCCGGATGGGTTACACCCCGCTCCATGTGGCCAGTCATTATGGAAACATCAAGCTGGTGAAGTTCT
  TGCTACAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCCTTGCACCAGGCAGCTCA
  ACAAGGACACACAGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAAATGAGGTCAGCTCGAAC
  GGAACCACACCTCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGACGTGCTCAAGGTGGTCA
  CAGATGAAACCAGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCTGAGACGGTGGACGAGAT
  CCTGGACGTGTCTGAAGATGAAGGAACTGCTCACATATCTATAATGGGTGACGAACTTGTCGGCTCGAAG
  GCTGAAAGGCGGGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTTTGTGCCGAAGCTAGACC
  AAGTGGTGGAGTCTCCAGCCATCCCAAGGATCCCTTGTGTCACACCTGAGACCGTTGTGATCCGATCTGA
  AGATCAGGAACAAGCATCTAAAGAATATGATGAGGATTCCCTCATTCCCAGCAGCCCGGCCACAGAGACC
  TCAGACAACATTAGCCCTGTGGCTAGCCCGGTCCACACAGGGTTCCTGGTGAGCTTCATGGTGGATGCCC
  GAGGGGGATCCATGAGAGGAAGCCGTCATAATGGCCTCAGGGTGGTGATCCCTCCCCGGACATGCGCAGC
  ACCCACTCGCATCACCTGCCGCCTCGTTAAGCCGCAGAAGCTGAACACGCCACCCCCACTGGCTGAGGAA
  GAGGGCCTAGCCAGCAGGATCATTGCCCTGGGACCCACGGGGGCCCAGTTCCTTAGCCCTGTGATCGTGG
  AAATCCCCCATTTTGCCTCCCATGGCCGTGGGGACCGTGAGCTGGTGGTTCTGAGGAGTGAAAACGGCTC
  TGTGTGGAAGGAGCACAAGAGTCGTTATGGGGAAAGCTACCTGGACCAGATCCTTAATGGCATGGATGAA
  GAGCTGGGGAGCCTGGAGGAGCTGGAGAAGAAACGTGTGTGCCGCATCATCACCACCGACTTCCCTCTGT
  ACTTTGTCATCATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAAGGGGGTTCCCTGAGAAG
  CAAGCTGGTACCGCTGGTACAGGCAACGTTCCCTGAAAACGCTGTGACCAAGAAAGTGAAGCTGGCTCTG
  CAGGCCCAGCCTGTCCCGGATGAGCTGGTGACCAAGCTCCTGGGTAACCAGGCCACATTCAGCCCCATTG
  TGACCGTGGAGCCGAGGCGTCGGAAGTTCCACCGTCCCATCGGGCTGCGAATCCCGCTCCCTCCCTCATG
  GACGGACAACCCCAGGGACAGTGGGGAGGGAGACACCACCAGCCTGCGCCTACTGTGTAGCGTCATCGGT
  GGAACTGACCAAGCCCAGTGGGAAGATATAACGGGAACAACCAAACTTATATACGCCAATGAGTGTGCCA
  ACTTTACCACCAATGTCTCAGCCAGGTTCTGGCTGTCAGACTGTCCCCGGACTGCGGAGGCTGTGCACTT
  TGCCACTCTCCTGTACAAAGAGCTCACAGCAGTCCCCTACATGGCTAAGTTTGTCATTTTTGCCAAGATG
  AACGACGCTCGGGAAGGACGGCTCCGTTGCTACTGCATGACGGATGACAAAGTGGACAAGACCCTGGAGC
  AGCATGAAAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTGGAAGGGATGCCTCTGTTTGC
  AGAACTCTCTGGGAACTTGGTTCCTGTCAAGAAAGCGGCCCAACAGCGGAGCTTCCATTTCCAGTCGTTT
  CGAGAGAATCGTCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCCAGGAGGGTTCTTGTCGT
  TCCTACGAAAGACAATGAAGTACGAGGACACACAGCACATCCTCTGTCACCTGAATATCACCATGCCCCC
  CTGCACCAAGGGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACCCCTGACCCTTCGATACAGCATT
  CTCAGCGAGTCGCGGCTGGGTTTTACCAGTGACACAGACCGTGTCGAAATGAGGATGGCTGTCATCAGAG
  AACACCTCGGCCTAAGCTGGGCAGAGCTGGCCCGGGAGCTGCAGTTCAGCGTGGAAGACATCAACCGGAT
  CCGTGTAGAAAACCCTAACTCCTTGTTGGACCAGAGTACAGCCTTGCTGACCCTCTGGGTGGACCGTGAA
  GGCGAAAATGCAAAGATGGAGAATTTGTACACAGCCCTGCGGAACATCGACAGGAGTGAGATTGTTAACA
  TGTTGGAGGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGAGCGGAGACATGGGGACCGGGAGTACTC
  ATTGTCACCCTCCCAGGTGAATGGTTACTCCTCGCTGCAGGACGAGCTGCTGTCCCCCGCCTCCCTGCAG
  TACGCTCTCCCCTCTCCACTGTGTGCAGACCAGTACTGGAACGAAGTGGCCGTCATAGACGCCATCCCCC
  TGGCGGCTACAGAGCATGACACCATGCTGGAGATGTCTGACATGCAGGTGTGGTCTGCGGGCCTCACACC
  CTCCCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGCAGCAAGGCTGAGGACTCTGACGCCATACCAGAG
  TGGAAGTTGGAAGGGGCACACTCAGAGGACACGCAGGGCCCGGAGCTGGGCTCTCAGGACCTGGTGGAGG
  ACGACACAGTGGATTCAGATGCCACAAATGGCCTGGCAGATTTGCTAGGCCAGCAGCGAGTTCACGCCCG
  AATCACAGACTCACCCTCAGTGAGGCAGGTGCTGGACAGAAGCCAGGCCAGAACACTGGACTGGGATAAA
  CAGGGTTCCACAGCGGTACACCCGCAAGAAGCCACACAGAGCTCCTGGCAAGAGGAGGTCACGCAGGGCC
  CACACTCATTCCAGAGAAGGATCACCACCATCCAAGGGCCGGAGCCTGGTGCGCTTCAGGAATACGAGCA
  GGTGCTGGTGTCTACCAGGGAGCATGTGCAGAGGGGGCCACCTGAGACCGGCAGCCCCAAAGCTGGCAAG
  GAACCTAGCCTGTGGGCACCTGAGAGCGCCTTCTCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTC
  CAGGAGAGCAGGTGACGGAGGAACAATTCACAGATGAACAGGGCAACATTGTTACCAAGAAGATCATTCG
  CAAAGTCGTCCGGCAGGTAGACTCGTCTGGTGCCATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGA
  GGGAGTGGACTCCAGCCGGACCTGATAGAGGGCAGGAAGGGGGCTCAGATAGTGAAGCGGGCCAGCCTGA
  AAAGGGGCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221570 representing NM_031158
  Red=Cloning site Green=Tags(s)
  
  MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLH
  KEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGAN
  QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTG
  FTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPL
  HCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAA
  HCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGH
  LPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMV
  KLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLL
  EHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGG
  SANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDA
  TTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSN
  GTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSK
  AERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATET
  SDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEE
  EGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDE
  ELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLAL
  QAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIG
  GTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKM
  NDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSF
  RENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSI
  LSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDRE
  GENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQ
  YALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPE
  WKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTLDWDK
  QGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGK
  EPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELR
  GSGLQPDLIEGRKGAQIVKRASLKRGKQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031158
• ORF Size: 5544 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031158.2
• RefSeq Size: 8179
• RefSeq ORF: 5547
• Locus ID: 11733
• Gene Summary: Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils. [UniProtKB/Swiss-Prot Function]