Rad18 (NM_021385) Mouse Tagged ORF Clone

CAT#: MG221590

Rad18 (GFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rad18
• Synonyms: 2810024C04Rik; Rad18sc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221590 representing NM_021385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTCCTGGCCGAGCCGCGATGGCCTCCGGGGTTGGCGGTGATGAAGACAATAGATGACTTGCTGC
  GCTGTGGGATTTGCTTTGAGTATTTCAACATTGCAGTGATAATCCCCCAGTGCTCTCACAACTATTGTTC
  ACTCTGTATAAGAAAGTTTTTATCCTATAAAACTCAGTGCCCAACTTGTTGCGTGGCAGTAACGGAGCCA
  GACCTGAGAAATAATCGCCTCTTAGATGAACTGGTAAAAAGCATGAATTTTGCACGGACTCACCTGTTGC
  AGTTTGCTTTAGAGTCACCACCCATATCTCCTGTGTCTTCCACCTCAAAGAAGGTTGTTGTTAAAGTGCA
  TAATGCTGACGCCGCCCAACACCCTGTCAAACAGGCGAACAGGTTAATGGATAAGTTCCTGATTAGAGAA
  ACTGGTGACTGTGTATTTGAGTTGTTGGGAAAAGAAAATGAGAGGAAATTCAGCCCTCAAAAAGAGCTAA
  GCACCTCTGCTGAGATTAAAGAGACAAGTCTCCTAGGAAAGCCGGTACTGGGGCTCTCGGATGCTAATGG
  TCCTGTGACTCCCTCTACATCCACTATGAAACTGGATACTAAAGTGTCTTGTCCTGTTTGTGGGGTCAGC
  ATTCCAGAAAATCATATCAATAAGCATTTAGACAGTTGTTTATCACGTGAAGAGAAGAAGGAGAGCCTGC
  GAAGTTCTGCTCACAAAAGGAAGCCGTTGCCCAAAACTGTATATAACTTGCTCTCTGATCGTGATTTAAA
  GAAAAAGCTGAAACAGTATGGCTTATCTGTTCAAGGAAACAAACAGCAGCTTATTAAAAGGCATCAGGAG
  TTTGTGCACATGTATAATGCCCAGTGCGATGCTTTGCATCCTAAATCAGCTGCTGAAATCGTCCAAGAAA
  TTGAAAGCATGGAGAAGACCAGGATGCGCCTTGAAGCAAGTAAACTCAATGAAAATGTCATGGTTTTTAC
  AAAGAACCAAACAGAGAAGGAAATTGAGGAAGTTCACAGTGAATATCGTAAAAAGCACCAGAATGCATTC
  CAGCTTCTGGTGGATCAGGCCAAAAAAGGGTATAAGAAAACTGGCAGAGTTTCACAAGCTGCAGCGATGA
  GAACAGATGAACCTGCAGAGACACTGCCGTCGATGAGAACAGATGAACCTGCAGAGACACTGCCGTCGAT
  GAGAACAGATGAACCTGCAGAGACACTGCCGTTGATGAGAGCAGATGAACCTGCAGAAACACTGCCGTCT
  GAGTGTATCGCACAAGAAGATAATGTGAGCTTCTCAGATACTGTCTCAGTAACAAACCACTTTCCTCAGC
  CCCAGCTGGACTCGCCAGGCCCGTCGGAGCCTGAGAGGCCGGATGATTCTTCTAGTTGTACTGATATTCT
  TTTCTCCTCGGACTCAGACTCATGCAATAGAAATGATCAAAACAGAGAAGTCAGCCCACAACAGACTCGC
  CGCACTAGAGCCAGTGAATGTGTTGAGATTGAACCAAGAAACAAGCGGAATAAGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221590 representing NM_021385
  Red=Cloning site Green=Tags(s)
  
  MEVLAEPRWPPGLAVMKTIDDLLRCGICFEYFNIAVIIPQCSHNYCSLCIRKFLSYKTQCPTCCVAVTEP
  DLRNNRLLDELVKSMNFARTHLLQFALESPPISPVSSTSKKVVVKVHNADAAQHPVKQANRLMDKFLIRE
  TGDCVFELLGKENERKFSPQKELSTSAEIKETSLLGKPVLGLSDANGPVTPSTSTMKLDTKVSCPVCGVS
  IPENHINKHLDSCLSREEKKESLRSSAHKRKPLPKTVYNLLSDRDLKKKLKQYGLSVQGNKQQLIKRHQE
  FVHMYNAQCDALHPKSAAEIVQEIESMEKTRMRLEASKLNENVMVFTKNQTEKEIEEVHSEYRKKHQNAF
  QLLVDQAKKGYKKTGRVSQAAAMRTDEPAETLPSMRTDEPAETLPSMRTDEPAETLPLMRADEPAETLPS
  ECIAQEDNVSFSDTVSVTNHFPQPQLDSPGPSEPERPDDSSSCTDILFSSDSDSCNRNDQNREVSPQQTR
  RTRASECVEIEPRNKRNKN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021385
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021385.2, NP_067360.2
• RefSeq Size: 2560
• RefSeq ORF: 1530
• Locus ID: 58186
• Gene Summary: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. [UniProtKB/Swiss-Prot Function]