Gnai1 (NM_010305) Mouse Tagged ORF Clone

CAT#: MG221647

Gnai1 (GFP-tagged) - Mouse guanine nucleotide binding protein (G protein) alpha inhibiting 1 (Gnai1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gnai1
• Synonyms: AU046200; Gialpha1; Gnai-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221647 representing NM_010305
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCACATTGAGCGCTGAGGACAAGGCGGCCGTGGAGCGCAGCAAGATGATCGACCGCAACCTCC
  GGGAGGACGGCGAGAAGGCGGCGCGGGAGGTCAAGCTGCTGCTGCTGGGTGCTGGGGAATCTGGAAAGAG
  TACCATTGTGAAGCAGATGAAGATTATCCACGAAGCCGGCTACTCGGAAGAGGAGTGTAAGCAGTACAAG
  GCAGTGGTCTACAGCAACACTATCCAGTCCATCATTGCCATCATTAGAGCCATGGGGAGGTTGAAAATCG
  ACTTCGGAGACTCTGCTCGGGCGGATGATGCTCGCCAACTTTTCGTGCTTGCTGGGGCTGCAGAAGAAGG
  CTTTATGACTGCAGAGCTCGCCGGTGTCATAAAGAGACTGTGGAAAGACAGTGGTGTGCAAGCCTGCTTC
  AACAGATCCCGGGAGTACCAGCTGAACGATTCGGCAGCGTACTATCTGAATGACTTGGACAGAATAGCAC
  AGCCAAATTACATCCCAACTCAGCAGGATGTCCTCAGAACCAGAGTGAAGACCACAGGGATTGTGGAAAC
  CCACTTTACCTTCAAAGATCTTCATTTTAAAATGTTTGACGTGGGAGGTCAGAGGTCAGAGCGGAAGAAG
  TGGATCCACTGCTTTGAAGGGGTGACCGCCATCATCTTCTGTGTGGCCCTGAGTGACTATGACCTGGTTC
  TTGCTGAAGATGAAGAAATGAACCGTATGCACGAGAGCATGAAGCTGTTCGATAGCATCTGTAACAACAA
  GTGGTTTACAGACACGTCCATCATCCTTTTCCTCAACAAGAAGGACCTCTTCGAAGAAAAAATAAAAAAG
  AGCCCCCTCACGATATGCTACCCAGAATATGCAGGCTCAAACACATATGAAGAAGCGGCCGCGTATATTC
  AGTGTCAGTTTGAAGACCTCAATAAAAGGAAGGACACAAAGGAAATTTACACCCACTTCACGTGCGCCAC
  AGATACGAAGAACGTGCAGTTCGTGTTCGATGCTGTAACAGACGTCATCATAAAGAATAACCTAAAAGAC
  TGTGGTCTCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221647 representing NM_010305
  Red=Cloning site Green=Tags(s)
  
  MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYK
  AVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACF
  NRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKK
  WIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKK
  SPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKD
  CGLF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010305
• ORF Size: 1062 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010305.1, NP_034435.1
• RefSeq Size: 3193
• RefSeq ORF: 1065
• Locus ID: 14677