Hcn1 (NM_010408) Mouse Tagged ORF Clone

CAT#: MG221659

Hcn1 (GFP-tagged) - Mouse hyperpolarization-activated cyclic nucleotide-gated K+ 1 (Hcn1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hcn1
• Synonyms: Bcng1; C630013B14Rik; HAC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221659 representing NM_010408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGCGGCGGCAAACCCAACTCCGCGTCCAACAGCCGCGACGATGGCAACAGCGTCTTCCCCTCCA
  AGGCGCCCGCGACGGGGCCGGTGGCGGCCGACAAGCGCCTGGGGACCCCGCCGGGGGGCGGCGCGGCCGG
  GAAGGAACATGGCAACTCCGTGTGCTTCAAGGTGGACGGCGGCGGAGGAGAGGAGCCGGCGGGCAGCTTC
  GAGGATGCCGAGGGGCCCCGGCGGCAGTATGGTTTCATGCAGAGGCAGTTCACCTCCATGCTGCAGCCTG
  GGGTCAACAAATTCTCCCTCCGCATGTTTGGGAGCCAGAAGGCGGTGGAGAAGGAGCAGGAAAGGGTTAA
  AACTGCAGGCTTCTGGATTATCCATCCGTACAGTGACTTCAGGTTTTATTGGGATTTAATCATGCTTATA
  ATGATGGTTGGAAATTTGGTCATCATACCAGTTGGAATCACGTTCTTCACAGAGCAGACGACAACACCGT
  GGATTATTTTCAACGTGGCATCCGATACTGTTTTCCTGTTGGACTTAATCATGAATTTTAGGACTGGGAC
  TGTCAATGAAGACAGCTCGGAAATCATCCTGGACCCTAAAGTGATCAAGATGAATTATTTAAAAAGCTGG
  TTTGTGGTGGACTTCATCTCATCGATCCCGGTGGATTATATCTTTCTCATTGTAGAGAAAGGGATGGACT
  CAGAAGTTTACAAGACAGCCAGAGCACTTCGTATCGTGAGGTTTACAAAAATTCTCAGTCTCTTGCGGTT
  ATTACGCCTTTCAAGGTTAATCAGATACATACACCAGTGGGAAGAGATATTCCACATGACCTATGACCTC
  GCCAGTGCTGTGGTGAGGATCTTCAACCTCATTGGCATGATGCTGCTTCTGTGCCACTGGGATGGCTGTC
  TTCAGTTCCTGGTTCCCCTGCTGCAGGACTTCCCACCAGATTGCTGGGTTTCTCTGAATGAAATGGTTAA
  TGATTCCTGGGGAAAACAATATTCCTACGCACTCTTCAAAGCTATGAGTCACATGCTGTGCATTGGTTAT
  GGCGCCCAAGCCCCTGTCAGCATGTCTGACCTCTGGATTACCATGCTGAGCATGATTGTGGGCGCCACCT
  GCTACGCAATGTTTGTTGGCCATGCCACAGCTTTGATCCAGTCTTTGGACTCTTCAAGGAGGCAGTATCA
  AGAGAAGTATAAGCAAGTAGAGCAATACATGTCATTCCACAAGTTACCAGCTGACATGCGCCAGAAGATA
  CATGATTACTATGAGCACCGATACCAAGGCAAGATCTTCGATGAAGAAAATATTCTCAGTGAGCTTAATG
  ATCCTCTGAGAGAGGAAATAGTCAACTTCAACTGCCGGAAACTGGTGGCAACTATGCCTCTTTTTGCTAA
  CGCCGATCCCAATTTCGTGACGGCCATGCTGAGCAAGCTGAGATTTGAGGTGTTCCAGCCCGGAGACTAT
  ATCATTCGAGAAGGAGCTGTGGGGAAGAAAATGTATTTCATCCAGCACGGTGTTGCTGGCGTTATCACCA
  AGTCCAGTAAAGAAATGAAGCTGACAGATGGCTCTTACTTCGGAGAGATATGCCTGCTGACCAAGGGCCG
  GCGCACTGCCAGTGTCCGAGCTGATACCTACTGTCGTCTTTACTCCCTTTCGGTGGACAATTTCAATGAG
  GTCTTGGAGGAATATCCAATGATGAGAAGAGCCTTTGAGACAGTTGCTATTGACCGACTCGATCGGATAG
  GCAAGAAAAACTCTATTCTCCTGCAGAAGTTCCAGAAGGATCTAAACACTGGTGTTTTCAACAACCAGGA
  GAACGAGATCCTGAAGCAGATCGTGAAGCATGACCGAGAGATGGTACAAGCTATCCCTCCAATCAACTAT
  CCTCAAATGACAGCCCTCAACTGCACATCTTCAACCACCACCCCAACCTCCCGCATGAGGACCCAATCTC
  CGCCAGTCTACACCGCAACCAGCCTGTCTCACAGCAATCTGCACTCACCCAGTCCCAGCACACAGACGCC
  CCAACCCTCAGCCATCCTTTCACCCTGCTCCTATACCACAGCAGTCTGCAGTCCTCCTATACAGAGCCCC
  CTGGCCACACGAACTTTCCATTATGCCTCTCCCACTGCGTCCCAGCTGTCACTCATGCAGCAGCCTCAGC
  AGCAACTACCGCAGTCCCAGGTACAGCAGACTCAGACTCAGACTCAGCAGCAGCAGCAGCAACAGCAGCA
  GCAGCAGCAGCAGCAACAGCAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG
  CAGCAGCAGCAGCAGCCACAGACACCTGGTAGCTCCACACCGAAAAATGAAGTGCACAAGAGCACACAAG
  CCCTTCATAACACCAACCTGACCAAAGAAGTCAGGCCCCTTTCCGCCTCGCAGCCTTCTCTGCCCCATGA
  GGTCTCCACTTTGATCTCCAGACCTCATCCCACTGTGGGCGAATCCCTGGCCTCTATCCCTCAACCCGTG
  GCAGCAGTCCACAGCACTGGCCTTCAGGCAGGGAGCAGGAGCACAGTGCCACAACGTGTCACCTTGTTCC
  GACAGATGTCCTCGGGAGCCATCCCCCCCAACCGAGGAGTGCCTCCAGCACCCCCTCCACCAGCAGCTGT
  GCAGAGAGAGTCTCCCTCAGTCCTAAATACAGACCCAGATGCAGAAAAACCCCGTTTTGCTTCGAATTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221659 representing NM_010408
  Red=Cloning site Green=Tags(s)
  
  MEGGGKPNSASNSRDDGNSVFPSKAPATGPVAADKRLGTPPGGGAAGKEHGNSVCFKVDGGGGEEPAGSF
  EDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLI
  MMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSW
  FVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDL
  ASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGY
  GAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKI
  HDYYEHRYQGKIFDEENILSELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDY
  IIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNE
  VLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIPPINY
  PQMTALNCTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPIQSP
  LATRTFHYASPTASQLSLMQQPQQQLPQSQVQQTQTQTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ
  QQQQQPQTPGSSTPKNEVHKSTQALHNTNLTKEVRPLSASQPSLPHEVSTLISRPHPTVGESLASIPQPV
  AAVHSTGLQAGSRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAVQRESPSVLNTDPDAEKPRFASNL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010408
• ORF Size: 2730 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010408.3, NP_034538.2
• RefSeq Size: 7911
• RefSeq ORF: 2733
• Locus ID: 15165