Amy2b (NM_001190404) Mouse Tagged ORF Clone

CAT#: MG221785

Amy2b (GFP-tagged) - Mouse amylase 2b (Amy2b) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Amy2b
• Synonyms: Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221785 representing NM_001190404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACCCACATACTTCAGATG
  GGAGGACTGCTATTGTCCACCTGTTCGAGTGGCGCTGGGTTGATATTGCCAAGGAATGTGAGCGATACTT
  AGCTCCTAAGGGATTTGGAGGGGTGCAGGTCTCTCCACCCAATGAAAATATTGTAATTCATAACCCATCA
  AGGCCTTGGTGGGAAAGATATCAACCAATCAGCTACAAAATTTGCACAAGGTCTGGAAATGAAGATGAAT
  TCAGAGACATGGTGACAAGGTGCAACAATGTTGGTGTCCGTATTTATGTGGATGCTGTCATTAACCACAT
  GTGTGGCTCAGGCAATCCTGCAGGAACAAGCAGTACCTGTGGAAGTTACCTCAATCCAAATAACAGGGAA
  TTCCCAGCAGTTCCATACTCTGCTTGGGACTTTAACGATAATAAATGTAATGGAGAAATTAGTAACTACA
  ATGATGCTTATCAGGTTATTGATCTGGGTGGTGAGGCAATTAAAGGTAGTGAGTACTTTGGAAATGGCCG
  TGTAACAGAATTCAAGTTTGGTGCAAAACTTGGCACAGTTATCCGCAAGTGGAATGGAGAGAAGATGTCC
  TATTTAAAGAACTGGGGAGAAGGTTGGGGTTTGGTGCCTTCTGACAGAGCCCTTGTGTTTGTGGACAACC
  ATGACAATCAGCGAGGACATGGTGCTGGAGGATCATCCATCCTGACATTCTGGGATGCTAGAATGTACAA
  AATGGCTGTTGGATTTATGTTGGCTCATCCTTATGGATTCACAAGAGTAATGTCAAGTTACCGTTGGAAT
  AGAAATTTCCAGAATGGAAAAGATCAGAATGACTGGATTGGACCACCTAATAACAATGGAGTAACAAAAG
  AAGTGACCATTAATGCAGACACTACTTGTGGCAATGACTGGGTCTGTGAACATAGATGGCGTCAAATAAG
  GAACATGGTTGCCTTCAGGAATGTAGTCAATGGTCAGCCTTTTGCAAACTGGTGGGATAATAACAGCAAC
  CAAGTAGCTTTTAGCAGAGGAAACAGAGGCTTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCA
  CTTTACAGACTGGTCTTCCTGCTGGCACATACTGTGATGTCATCTCTGGAGATAAGGTCGATGGCAATTG
  CACTGGACTTAAAGTAAATGTTGGCAGTGATGGTAAAGCTCACTTTTCCATTAGTAACTCTGCTGAGGAC
  CCATTTATTGCAATCCATGCTGACTCAAAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221785 representing NM_001190404
  Red=Cloning site Green=Tags(s)
  
  MKFVLLLSLIGFCWAQYDPHTSDGRTAIVHLFEWRWVDIAKECERYLAPKGFGGVQVSPPNENIVIHNPS
  RPWWERYQPISYKICTRSGNEDEFRDMVTRCNNVGVRIYVDAVINHMCGSGNPAGTSSTCGSYLNPNNRE
  FPAVPYSAWDFNDNKCNGEISNYNDAYQVIDLGGEAIKGSEYFGNGRVTEFKFGAKLGTVIRKWNGEKMS
  YLKNWGEGWGLVPSDRALVFVDNHDNQRGHGAGGSSILTFWDARMYKMAVGFMLAHPYGFTRVMSSYRWN
  RNFQNGKDQNDWIGPPNNNGVTKEVTINADTTCGNDWVCEHRWRQIRNMVAFRNVVNGQPFANWWDNNSN
  QVAFSRGNRGFIVFNNDDWALSATLQTGLPAGTYCDVISGDKVDGNCTGLKVNVGSDGKAHFSISNSAED
  PFIAIHADSKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190404
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001190404.1, NP_001177333.1
• RefSeq Size: 1347
• RefSeq ORF: 1296
• Locus ID: 545562
• Gene Summary: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The mouse genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. At least one mouse strain has a non-functional allele of this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2011]